MicroRNA-590-3p enhances the radioresistance in glioblastoma cells by targeting LRIG1

microRNA (miR)-590 has been found to serve potential roles in cancer development; however, the expression and function of miR-590 in human gliomas remains to be elucidated. The present study aimed to investigate the expression of miR-590 in human glioma tissues and radioresistant human glioblastoma...

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Veröffentlicht in:Experimental and therapeutic medicine 2017-08, Vol.14 (2), p.1818-1824
Hauptverfasser: Chen, Long, Wang, Wenhua, Zhu, Shengqiang, Jin, Xuegang, Wang, Jian, Zhu, Jianfang, Zhou, Youxin
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container_issue 2
container_start_page 1818
container_title Experimental and therapeutic medicine
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creator Chen, Long
Wang, Wenhua
Zhu, Shengqiang
Jin, Xuegang
Wang, Jian
Zhu, Jianfang
Zhou, Youxin
description microRNA (miR)-590 has been found to serve potential roles in cancer development; however, the expression and function of miR-590 in human gliomas remains to be elucidated. The present study aimed to investigate the expression of miR-590 in human glioma tissues and radioresistant human glioblastoma cells (U251R), and to determine the effect and related molecular mechanism of miR-590-3p on the radiosensitivity of U251R cells in vitro. The results from reverse transcription-quantitative polymerase chain reaction indicated that miR-590-3p was upregulated in human glioma tissues and radioresistant human glioblastoma cells, and that miR-590-3p expression was higher in high grade than in low grade gliomas. In vitro experiments revealed that the miR-590-3p inhibitor enhanced the radiosensitivity of U251R cells by suppressing cell viability, decreasing colony formation capacity and increasing cell apoptosis rate, as demonstrated by MTT, colony formation and flow cytometry analyses. A luciferase reporter assay demonstrated that leucine-rich repeats and immunoglobulin-like domains protein 1 (LRIG1) was a direct target of miR-590-3p. Furthermore, it was demonstrated that the effect of miR-590-3p suppression on cell viability, colony formation capacity and cell apoptosis rate was attenuated by the knockdown of LRIG1 in the U251R cells. In conclusion, the present study revealed that miR-590-3p was upregulated in human glioma tissues and radioresistant human glioblastoma cells, and miR-590-3p contributes to the radioresistance of human glioblastoma cells by directly targeting LRIG1. These findings may provide potential therapeutic strategies to prevent radioresistance in human gliomas.
doi_str_mv 10.3892/etm.2017.4697
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The present study aimed to investigate the expression of miR-590 in human glioma tissues and radioresistant human glioblastoma cells (U251R), and to determine the effect and related molecular mechanism of miR-590-3p on the radiosensitivity of U251R cells in vitro. The results from reverse transcription-quantitative polymerase chain reaction indicated that miR-590-3p was upregulated in human glioma tissues and radioresistant human glioblastoma cells, and that miR-590-3p expression was higher in high grade than in low grade gliomas. In vitro experiments revealed that the miR-590-3p inhibitor enhanced the radiosensitivity of U251R cells by suppressing cell viability, decreasing colony formation capacity and increasing cell apoptosis rate, as demonstrated by MTT, colony formation and flow cytometry analyses. A luciferase reporter assay demonstrated that leucine-rich repeats and immunoglobulin-like domains protein 1 (LRIG1) was a direct target of miR-590-3p. Furthermore, it was demonstrated that the effect of miR-590-3p suppression on cell viability, colony formation capacity and cell apoptosis rate was attenuated by the knockdown of LRIG1 in the U251R cells. In conclusion, the present study revealed that miR-590-3p was upregulated in human glioma tissues and radioresistant human glioblastoma cells, and miR-590-3p contributes to the radioresistance of human glioblastoma cells by directly targeting LRIG1. These findings may provide potential therapeutic strategies to prevent radioresistance in human gliomas.</description><identifier>ISSN: 1792-0981</identifier><identifier>EISSN: 1792-1015</identifier><identifier>DOI: 10.3892/etm.2017.4697</identifier><identifier>PMID: 28810655</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Apoptosis ; Brain cancer ; Cancer therapies ; Chemotherapy ; glioblastoma ; Glioblastomas ; Glioma ; leucine-rich repeats and immunoglobulin-like domains protein 1 ; Medical prognosis ; MicroRNA ; microRNA-590-3p ; MicroRNAs ; Prognosis ; Radiation therapy ; radiosensitivity ; Studies ; Tumors</subject><ispartof>Experimental and therapeutic medicine, 2017-08, Vol.14 (2), p.1818-1824</ispartof><rights>Copyright © 2017, Spandidos Publications</rights><rights>COPYRIGHT 2017 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2017</rights><rights>Copyright © 2017, Spandidos Publications 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c512t-30836b275fa7303aef690382d1d160f8696943c60481ecbecb17d7d0bf4896173</citedby><cites>FETCH-LOGICAL-c512t-30836b275fa7303aef690382d1d160f8696943c60481ecbecb17d7d0bf4896173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5526158/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5526158/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28810655$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Long</creatorcontrib><creatorcontrib>Wang, Wenhua</creatorcontrib><creatorcontrib>Zhu, Shengqiang</creatorcontrib><creatorcontrib>Jin, Xuegang</creatorcontrib><creatorcontrib>Wang, Jian</creatorcontrib><creatorcontrib>Zhu, Jianfang</creatorcontrib><creatorcontrib>Zhou, Youxin</creatorcontrib><title>MicroRNA-590-3p enhances the radioresistance in glioblastoma cells by targeting LRIG1</title><title>Experimental and therapeutic medicine</title><addtitle>Exp Ther Med</addtitle><description>microRNA (miR)-590 has been found to serve potential roles in cancer development; however, the expression and function of miR-590 in human gliomas remains to be elucidated. 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Furthermore, it was demonstrated that the effect of miR-590-3p suppression on cell viability, colony formation capacity and cell apoptosis rate was attenuated by the knockdown of LRIG1 in the U251R cells. In conclusion, the present study revealed that miR-590-3p was upregulated in human glioma tissues and radioresistant human glioblastoma cells, and miR-590-3p contributes to the radioresistance of human glioblastoma cells by directly targeting LRIG1. These findings may provide potential therapeutic strategies to prevent radioresistance in human gliomas.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>28810655</pmid><doi>10.3892/etm.2017.4697</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record>
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subjects Apoptosis
Brain cancer
Cancer therapies
Chemotherapy
glioblastoma
Glioblastomas
Glioma
leucine-rich repeats and immunoglobulin-like domains protein 1
Medical prognosis
MicroRNA
microRNA-590-3p
MicroRNAs
Prognosis
Radiation therapy
radiosensitivity
Studies
Tumors
title MicroRNA-590-3p enhances the radioresistance in glioblastoma cells by targeting LRIG1
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