Long noncoding RNA CRNDE stabilized by hnRNPUL2 accelerates cell proliferation and migration in colorectal carcinoma via activating Ras/MAPK signaling pathways
Recent studies have furthered our understanding of the function of long noncoding RNAs (lncRNAs) in numerous biological processes, including cancer. This study investigated the expression of a novel lncRNA, colorectal neoplasia differentially expressed ( CRNDE ), in colorectal carcinoma (CRC) tissue...
Gespeichert in:
| Veröffentlicht in: | Cell death & disease 2017-06, Vol.8 (6), p.e2862-e2862 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e2862 |
|---|---|
| container_issue | 6 |
| container_start_page | e2862 |
| container_title | Cell death & disease |
| container_volume | 8 |
| creator | Jiang, Huijuan Wang, Yiqing Ai, Meiling Wang, Haowei Duan, Zhijiao Wang, Huanan Zhao, Li Yu, Jiang Ding, Yanqing Wang, Shuang |
| description | Recent studies have furthered our understanding of the function of long noncoding RNAs (lncRNAs) in numerous biological processes, including cancer. This study investigated the expression of a novel lncRNA, colorectal neoplasia differentially expressed (
CRNDE
), in colorectal carcinoma (CRC) tissues and cells by real-time RT-PCR and
in situ
hybridization, and its biological function using a series of
in vitro
and
in vivo
experiments to determine its potential as a prognostic marker and therapeutic target.
CRNDE
was found to be upregulated in primary CRC tissues and cells (
P |
| doi_str_mv | 10.1038/cddis.2017.258 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5520914</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1907322795</sourcerecordid><originalsourceid>FETCH-LOGICAL-c524t-4e042e57cf1378e7acbebfc9310ea6c3ab0c5f639c60b1af7d86d5f08955502d3</originalsourceid><addsrcrecordid>eNptkU2P0zAQhi0EYlfLXjkiS1y4tPVHnMQXpKq7fIhSVhV7tiaOk3qV2MVOi8qf4a_i0LIqCF88nnn8jscvQi8pmVLCy5muaxunjNBiykT5BF0yktFJVpby6Vl8ga5jfCBpcU6YyJ-jC1YKmWWEX6KfS-9a7LzTvrYpWq_meLFe3dziOEBlO_vD1Lg64I1br-7ulwyD1qYzAQYTcYo6vA2-s82Ysd5hcDXubXs6WYe173wweoAOawjaOt8D3ltIQoPdJ2xsCnH2eX73CUfbOujG1BaGzXc4xBfoWQNdNNen_Qrdv7v9uvgwWX55_3ExX060YNkwyQzJmBGFbigvSlOArkzVaMkpMZBrDhXRosm51DmpKDRFXea1aEgphRCE1fwKvT3qbndVb2pt3BCgU9tgewgH5cGqvyvOblTr90oIRiTNksCbk0Dw33YmDqq3cfwgcMbvoqKSFJyxQoqEvv4HffC7kAYfKVrIXHI-UtMjpYOPMZjm8TGUqNF-9dt-Ndqvkv3pwqvzER7xP2YnYHYEYiq51oSzvv-X_AWf476a</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1917969335</pqid></control><display><type>article</type><title>Long noncoding RNA CRNDE stabilized by hnRNPUL2 accelerates cell proliferation and migration in colorectal carcinoma via activating Ras/MAPK signaling pathways</title><source>MEDLINE</source><source>Nature Free</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Springer Nature OA Free Journals</source><creator>Jiang, Huijuan ; Wang, Yiqing ; Ai, Meiling ; Wang, Haowei ; Duan, Zhijiao ; Wang, Huanan ; Zhao, Li ; Yu, Jiang ; Ding, Yanqing ; Wang, Shuang</creator><creatorcontrib>Jiang, Huijuan ; Wang, Yiqing ; Ai, Meiling ; Wang, Haowei ; Duan, Zhijiao ; Wang, Huanan ; Zhao, Li ; Yu, Jiang ; Ding, Yanqing ; Wang, Shuang</creatorcontrib><description>Recent studies have furthered our understanding of the function of long noncoding RNAs (lncRNAs) in numerous biological processes, including cancer. This study investigated the expression of a novel lncRNA, colorectal neoplasia differentially expressed (
CRNDE
), in colorectal carcinoma (CRC) tissues and cells by real-time RT-PCR and
in situ
hybridization, and its biological function using a series of
in vitro
and
in vivo
experiments to determine its potential as a prognostic marker and therapeutic target.
CRNDE
was found to be upregulated in primary CRC tissues and cells (
P
<0.05), and the upregulation of
CRNDE
expression is a powerful predictor of advanced TNM stage (
P
<0.05) and poor prognosis for CRC patients (
P
=0.002). The promoting effects of
CRNDE
on the cell proliferation, cell cycling and metastasis of CRC cells were confirmed both
in vitro
and
in vivo
by gain-of-function and loss-of-function experiments. Mechanistically, it was demonstrated that
CRNDE
could form a functional complex with heterogeneous nuclear ribonucleoprotein U-like 2 protein (hnRNPUL2) and direct the transport of hnRNPUL2 between the nucleus and cytoplasm. hnRNPUL2 that was accumulated in the cytoplasm could interact with
CRNDE
both physically and functionally, increasing the stability of
CRNDE
RNA. Moreover, gene expression profile data showed that
CRNDE
depletion in cells downregulated a series of genes involved in the Ras/mitogen-activated protein kinase signaling pathways. Collectively, these findings provide novel insights into the function and mechanism of lncRNA
CRNDE
in the pathogenesis of CRC and highlight its potential as a therapeutic target for CRC intervention.</description><identifier>ISSN: 2041-4889</identifier><identifier>EISSN: 2041-4889</identifier><identifier>DOI: 10.1038/cddis.2017.258</identifier><identifier>PMID: 28594403</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/89 ; 38/1 ; 631/337/384/2568 ; 631/80/86 ; 692/699/67/1504/1885 ; 692/699/67/322 ; 82/51 ; 82/80 ; Animals ; Antibodies ; Biochemistry ; Biomedical and Life Sciences ; Cell Biology ; Cell Culture ; Cell growth ; Cell Line, Tumor ; Cell Movement ; Cell Proliferation ; Colorectal cancer ; Colorectal carcinoma ; Colorectal Neoplasms - genetics ; Colorectal Neoplasms - metabolism ; Colorectal Neoplasms - pathology ; Cytoplasm ; Extracellular Signal-Regulated MAP Kinases - genetics ; Extracellular Signal-Regulated MAP Kinases - metabolism ; Female ; Gene expression ; Heterogeneous-Nuclear Ribonucleoproteins - genetics ; Heterogeneous-Nuclear Ribonucleoproteins - metabolism ; Humans ; Immunology ; Kinases ; Life Sciences ; Male ; MAP kinase ; MAP Kinase Signaling System ; Metastases ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Original ; original-article ; Polymerase chain reaction ; Protein kinase ; Proto-Oncogene Proteins p21(ras) - genetics ; Proto-Oncogene Proteins p21(ras) - metabolism ; Ras protein ; Ribonucleic acid ; RNA ; RNA, Long Noncoding - genetics ; RNA, Long Noncoding - metabolism ; RNA, Neoplasm - genetics ; RNA, Neoplasm - metabolism ; Signal transduction</subject><ispartof>Cell death & disease, 2017-06, Vol.8 (6), p.e2862-e2862</ispartof><rights>The Author(s) 2017</rights><rights>Copyright Nature Publishing Group Jun 2017</rights><rights>Copyright © 2017 The Author(s) 2017 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c524t-4e042e57cf1378e7acbebfc9310ea6c3ab0c5f639c60b1af7d86d5f08955502d3</citedby><cites>FETCH-LOGICAL-c524t-4e042e57cf1378e7acbebfc9310ea6c3ab0c5f639c60b1af7d86d5f08955502d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5520914/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5520914/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28594403$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jiang, Huijuan</creatorcontrib><creatorcontrib>Wang, Yiqing</creatorcontrib><creatorcontrib>Ai, Meiling</creatorcontrib><creatorcontrib>Wang, Haowei</creatorcontrib><creatorcontrib>Duan, Zhijiao</creatorcontrib><creatorcontrib>Wang, Huanan</creatorcontrib><creatorcontrib>Zhao, Li</creatorcontrib><creatorcontrib>Yu, Jiang</creatorcontrib><creatorcontrib>Ding, Yanqing</creatorcontrib><creatorcontrib>Wang, Shuang</creatorcontrib><title>Long noncoding RNA CRNDE stabilized by hnRNPUL2 accelerates cell proliferation and migration in colorectal carcinoma via activating Ras/MAPK signaling pathways</title><title>Cell death & disease</title><addtitle>Cell Death Dis</addtitle><addtitle>Cell Death Dis</addtitle><description>Recent studies have furthered our understanding of the function of long noncoding RNAs (lncRNAs) in numerous biological processes, including cancer. This study investigated the expression of a novel lncRNA, colorectal neoplasia differentially expressed (
CRNDE
), in colorectal carcinoma (CRC) tissues and cells by real-time RT-PCR and
in situ
hybridization, and its biological function using a series of
in vitro
and
in vivo
experiments to determine its potential as a prognostic marker and therapeutic target.
CRNDE
was found to be upregulated in primary CRC tissues and cells (
P
<0.05), and the upregulation of
CRNDE
expression is a powerful predictor of advanced TNM stage (
P
<0.05) and poor prognosis for CRC patients (
P
=0.002). The promoting effects of
CRNDE
on the cell proliferation, cell cycling and metastasis of CRC cells were confirmed both
in vitro
and
in vivo
by gain-of-function and loss-of-function experiments. Mechanistically, it was demonstrated that
CRNDE
could form a functional complex with heterogeneous nuclear ribonucleoprotein U-like 2 protein (hnRNPUL2) and direct the transport of hnRNPUL2 between the nucleus and cytoplasm. hnRNPUL2 that was accumulated in the cytoplasm could interact with
CRNDE
both physically and functionally, increasing the stability of
CRNDE
RNA. Moreover, gene expression profile data showed that
CRNDE
depletion in cells downregulated a series of genes involved in the Ras/mitogen-activated protein kinase signaling pathways. Collectively, these findings provide novel insights into the function and mechanism of lncRNA
CRNDE
in the pathogenesis of CRC and highlight its potential as a therapeutic target for CRC intervention.</description><subject>13/89</subject><subject>38/1</subject><subject>631/337/384/2568</subject><subject>631/80/86</subject><subject>692/699/67/1504/1885</subject><subject>692/699/67/322</subject><subject>82/51</subject><subject>82/80</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Cell Biology</subject><subject>Cell Culture</subject><subject>Cell growth</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement</subject><subject>Cell Proliferation</subject><subject>Colorectal cancer</subject><subject>Colorectal carcinoma</subject><subject>Colorectal Neoplasms - genetics</subject><subject>Colorectal Neoplasms - metabolism</subject><subject>Colorectal Neoplasms - pathology</subject><subject>Cytoplasm</subject><subject>Extracellular Signal-Regulated MAP Kinases - genetics</subject><subject>Extracellular Signal-Regulated MAP Kinases - metabolism</subject><subject>Female</subject><subject>Gene expression</subject><subject>Heterogeneous-Nuclear Ribonucleoproteins - genetics</subject><subject>Heterogeneous-Nuclear Ribonucleoproteins - metabolism</subject><subject>Humans</subject><subject>Immunology</subject><subject>Kinases</subject><subject>Life Sciences</subject><subject>Male</subject><subject>MAP kinase</subject><subject>MAP Kinase Signaling System</subject><subject>Metastases</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Nude</subject><subject>Original</subject><subject>original-article</subject><subject>Polymerase chain reaction</subject><subject>Protein kinase</subject><subject>Proto-Oncogene Proteins p21(ras) - genetics</subject><subject>Proto-Oncogene Proteins p21(ras) - metabolism</subject><subject>Ras protein</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA, Long Noncoding - genetics</subject><subject>RNA, Long Noncoding - metabolism</subject><subject>RNA, Neoplasm - genetics</subject><subject>RNA, Neoplasm - metabolism</subject><subject>Signal transduction</subject><issn>2041-4889</issn><issn>2041-4889</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNptkU2P0zAQhi0EYlfLXjkiS1y4tPVHnMQXpKq7fIhSVhV7tiaOk3qV2MVOi8qf4a_i0LIqCF88nnn8jscvQi8pmVLCy5muaxunjNBiykT5BF0yktFJVpby6Vl8ga5jfCBpcU6YyJ-jC1YKmWWEX6KfS-9a7LzTvrYpWq_meLFe3dziOEBlO_vD1Lg64I1br-7ulwyD1qYzAQYTcYo6vA2-s82Ysd5hcDXubXs6WYe173wweoAOawjaOt8D3ltIQoPdJ2xsCnH2eX73CUfbOujG1BaGzXc4xBfoWQNdNNen_Qrdv7v9uvgwWX55_3ExX060YNkwyQzJmBGFbigvSlOArkzVaMkpMZBrDhXRosm51DmpKDRFXea1aEgphRCE1fwKvT3qbndVb2pt3BCgU9tgewgH5cGqvyvOblTr90oIRiTNksCbk0Dw33YmDqq3cfwgcMbvoqKSFJyxQoqEvv4HffC7kAYfKVrIXHI-UtMjpYOPMZjm8TGUqNF-9dt-Ndqvkv3pwqvzER7xP2YnYHYEYiq51oSzvv-X_AWf476a</recordid><startdate>20170608</startdate><enddate>20170608</enddate><creator>Jiang, Huijuan</creator><creator>Wang, Yiqing</creator><creator>Ai, Meiling</creator><creator>Wang, Haowei</creator><creator>Duan, Zhijiao</creator><creator>Wang, Huanan</creator><creator>Zhao, Li</creator><creator>Yu, Jiang</creator><creator>Ding, Yanqing</creator><creator>Wang, Shuang</creator><general>Nature Publishing Group UK</general><general>Springer Nature B.V</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170608</creationdate><title>Long noncoding RNA CRNDE stabilized by hnRNPUL2 accelerates cell proliferation and migration in colorectal carcinoma via activating Ras/MAPK signaling pathways</title><author>Jiang, Huijuan ; Wang, Yiqing ; Ai, Meiling ; Wang, Haowei ; Duan, Zhijiao ; Wang, Huanan ; Zhao, Li ; Yu, Jiang ; Ding, Yanqing ; Wang, Shuang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c524t-4e042e57cf1378e7acbebfc9310ea6c3ab0c5f639c60b1af7d86d5f08955502d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>13/89</topic><topic>38/1</topic><topic>631/337/384/2568</topic><topic>631/80/86</topic><topic>692/699/67/1504/1885</topic><topic>692/699/67/322</topic><topic>82/51</topic><topic>82/80</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Cell Biology</topic><topic>Cell Culture</topic><topic>Cell growth</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement</topic><topic>Cell Proliferation</topic><topic>Colorectal cancer</topic><topic>Colorectal carcinoma</topic><topic>Colorectal Neoplasms - genetics</topic><topic>Colorectal Neoplasms - metabolism</topic><topic>Colorectal Neoplasms - pathology</topic><topic>Cytoplasm</topic><topic>Extracellular Signal-Regulated MAP Kinases - genetics</topic><topic>Extracellular Signal-Regulated MAP Kinases - metabolism</topic><topic>Female</topic><topic>Gene expression</topic><topic>Heterogeneous-Nuclear Ribonucleoproteins - genetics</topic><topic>Heterogeneous-Nuclear Ribonucleoproteins - metabolism</topic><topic>Humans</topic><topic>Immunology</topic><topic>Kinases</topic><topic>Life Sciences</topic><topic>Male</topic><topic>MAP kinase</topic><topic>MAP Kinase Signaling System</topic><topic>Metastases</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Nude</topic><topic>Original</topic><topic>original-article</topic><topic>Polymerase chain reaction</topic><topic>Protein kinase</topic><topic>Proto-Oncogene Proteins p21(ras) - genetics</topic><topic>Proto-Oncogene Proteins p21(ras) - metabolism</topic><topic>Ras protein</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA, Long Noncoding - genetics</topic><topic>RNA, Long Noncoding - metabolism</topic><topic>RNA, Neoplasm - genetics</topic><topic>RNA, Neoplasm - metabolism</topic><topic>Signal transduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jiang, Huijuan</creatorcontrib><creatorcontrib>Wang, Yiqing</creatorcontrib><creatorcontrib>Ai, Meiling</creatorcontrib><creatorcontrib>Wang, Haowei</creatorcontrib><creatorcontrib>Duan, Zhijiao</creatorcontrib><creatorcontrib>Wang, Huanan</creatorcontrib><creatorcontrib>Zhao, Li</creatorcontrib><creatorcontrib>Yu, Jiang</creatorcontrib><creatorcontrib>Ding, Yanqing</creatorcontrib><creatorcontrib>Wang, Shuang</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cell death & disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jiang, Huijuan</au><au>Wang, Yiqing</au><au>Ai, Meiling</au><au>Wang, Haowei</au><au>Duan, Zhijiao</au><au>Wang, Huanan</au><au>Zhao, Li</au><au>Yu, Jiang</au><au>Ding, Yanqing</au><au>Wang, Shuang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Long noncoding RNA CRNDE stabilized by hnRNPUL2 accelerates cell proliferation and migration in colorectal carcinoma via activating Ras/MAPK signaling pathways</atitle><jtitle>Cell death & disease</jtitle><stitle>Cell Death Dis</stitle><addtitle>Cell Death Dis</addtitle><date>2017-06-08</date><risdate>2017</risdate><volume>8</volume><issue>6</issue><spage>e2862</spage><epage>e2862</epage><pages>e2862-e2862</pages><issn>2041-4889</issn><eissn>2041-4889</eissn><abstract>Recent studies have furthered our understanding of the function of long noncoding RNAs (lncRNAs) in numerous biological processes, including cancer. This study investigated the expression of a novel lncRNA, colorectal neoplasia differentially expressed (
CRNDE
), in colorectal carcinoma (CRC) tissues and cells by real-time RT-PCR and
in situ
hybridization, and its biological function using a series of
in vitro
and
in vivo
experiments to determine its potential as a prognostic marker and therapeutic target.
CRNDE
was found to be upregulated in primary CRC tissues and cells (
P
<0.05), and the upregulation of
CRNDE
expression is a powerful predictor of advanced TNM stage (
P
<0.05) and poor prognosis for CRC patients (
P
=0.002). The promoting effects of
CRNDE
on the cell proliferation, cell cycling and metastasis of CRC cells were confirmed both
in vitro
and
in vivo
by gain-of-function and loss-of-function experiments. Mechanistically, it was demonstrated that
CRNDE
could form a functional complex with heterogeneous nuclear ribonucleoprotein U-like 2 protein (hnRNPUL2) and direct the transport of hnRNPUL2 between the nucleus and cytoplasm. hnRNPUL2 that was accumulated in the cytoplasm could interact with
CRNDE
both physically and functionally, increasing the stability of
CRNDE
RNA. Moreover, gene expression profile data showed that
CRNDE
depletion in cells downregulated a series of genes involved in the Ras/mitogen-activated protein kinase signaling pathways. Collectively, these findings provide novel insights into the function and mechanism of lncRNA
CRNDE
in the pathogenesis of CRC and highlight its potential as a therapeutic target for CRC intervention.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>28594403</pmid><doi>10.1038/cddis.2017.258</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2041-4889 |
| ispartof | Cell death & disease, 2017-06, Vol.8 (6), p.e2862-e2862 |
| issn | 2041-4889 2041-4889 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5520914 |
| source | MEDLINE; Nature Free; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Springer Nature OA Free Journals |
| subjects | 13/89 38/1 631/337/384/2568 631/80/86 692/699/67/1504/1885 692/699/67/322 82/51 82/80 Animals Antibodies Biochemistry Biomedical and Life Sciences Cell Biology Cell Culture Cell growth Cell Line, Tumor Cell Movement Cell Proliferation Colorectal cancer Colorectal carcinoma Colorectal Neoplasms - genetics Colorectal Neoplasms - metabolism Colorectal Neoplasms - pathology Cytoplasm Extracellular Signal-Regulated MAP Kinases - genetics Extracellular Signal-Regulated MAP Kinases - metabolism Female Gene expression Heterogeneous-Nuclear Ribonucleoproteins - genetics Heterogeneous-Nuclear Ribonucleoproteins - metabolism Humans Immunology Kinases Life Sciences Male MAP kinase MAP Kinase Signaling System Metastases Mice Mice, Inbred BALB C Mice, Nude Original original-article Polymerase chain reaction Protein kinase Proto-Oncogene Proteins p21(ras) - genetics Proto-Oncogene Proteins p21(ras) - metabolism Ras protein Ribonucleic acid RNA RNA, Long Noncoding - genetics RNA, Long Noncoding - metabolism RNA, Neoplasm - genetics RNA, Neoplasm - metabolism Signal transduction |
| title | Long noncoding RNA CRNDE stabilized by hnRNPUL2 accelerates cell proliferation and migration in colorectal carcinoma via activating Ras/MAPK signaling pathways |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T20%3A20%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Long%20noncoding%20RNA%20CRNDE%20stabilized%20by%20hnRNPUL2%20accelerates%20cell%20proliferation%20and%20migration%20in%20colorectal%20carcinoma%20via%20activating%20Ras/MAPK%20signaling%20pathways&rft.jtitle=Cell%20death%20&%20disease&rft.au=Jiang,%20Huijuan&rft.date=2017-06-08&rft.volume=8&rft.issue=6&rft.spage=e2862&rft.epage=e2862&rft.pages=e2862-e2862&rft.issn=2041-4889&rft.eissn=2041-4889&rft_id=info:doi/10.1038/cddis.2017.258&rft_dat=%3Cproquest_pubme%3E1907322795%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1917969335&rft_id=info:pmid/28594403&rfr_iscdi=true |