The use of fluorescently-tagged apoptolidins in cellular uptake and response studies

The use of fluorescently-tagged apoptolidins in cellular uptake and response studies

The apoptolidins are glycomacrolide microbial metabolites reported to be selectively cytotoxic against tumor cells. Using fluorescently tagged active derivatives we demonstrate selective uptake of these four tagged glycomacrolides in cancer cells over healthy human blood cells. We also demonstrate t...

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Veröffentlicht in:Journal of antibiotics 2016-04, Vol.69 (4), p.327-330
Hauptverfasser: Chong, Katherine M, Leelatian, Nalin, Deguire, Sean M, Brockman, Asa A, Earl, David, Ihrie, Rebecca A, Irish, Jonathan M, Bachmann, Brian O, Sulikowski, Gary A
Format: Artikel
Sprache:eng
Schlagworte:
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A549 Cells
Bacteriology
Biological Transport
Biomedical and Life Sciences
Bioorganic Chemistry
Cell Line, Tumor
Flow Cytometry
Fluorescent Dyes
Humans
Life Sciences
Macrolides - chemistry
Macrolides - metabolism
Medicinal Chemistry
Metabolites
Microbiology
Organic Chemistry
original-article
Staining and Labeling
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Zusammenfassung:The apoptolidins are glycomacrolide microbial metabolites reported to be selectively cytotoxic against tumor cells. Using fluorescently tagged active derivatives we demonstrate selective uptake of these four tagged glycomacrolides in cancer cells over healthy human blood cells. We also demonstrate the utility of these five fluorescently tagged glycomacrolides in fluorescent flow cytometry to monitor cellular uptake of the six glycomacrolides and cellular response.
ISSN:0021-8820
1881-1469
DOI:10.1038/ja.2016.22