Non‐contact luminescence lifetime cryothermometry for macromolecular crystallography

Non‐contact luminescence lifetime cryothermometry for macromolecular crystallography

Temperature is a very important parameter when aiming to minimize radiation damage to biological samples during experiments that utilize intense ionizing radiation. A novel technique for remote, non‐contact, in situ monitoring of the protein crystal temperature has been developed for the new I23 bea...

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Veröffentlicht in:Journal of synchrotron radiation 2017-05, Vol.24 (3), p.636-645
Hauptverfasser: Mykhaylyk, V. B., Wagner, A., Kraus, H.
Format: Artikel
Sprache:eng
Schlagworte:
bismuth germanate (BGO)
cryogenic thermometry
Crystals
Decay
Luminescence
luminescence lifetime
Particle physics
protein crystalography
Proteins
Remote monitoring
Research Papers
Scintillation
Sensors
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Zusammenfassung:Temperature is a very important parameter when aiming to minimize radiation damage to biological samples during experiments that utilize intense ionizing radiation. A novel technique for remote, non‐contact, in situ monitoring of the protein crystal temperature has been developed for the new I23 beamline at the Diamond Light Source, a facility dedicated to macromolecular crystallography (MX) with long‐wavelength X‐rays. The temperature is derived from the temperature‐dependent decay time constant of luminescence from a minuscule scintillation sensor (
ISSN:1600-5775
0909-0495
1600-5775
DOI:10.1107/S1600577517003484