Standard Dyes for Total Protein Staining in Gel-Based Proteomic Analysis

Standard Dyes for Total Protein Staining in Gel-Based Proteomic Analysis

Staining of two-dimensional gels is a primary concern in proteomic studies using two-dimensional gel electrophoresis with respect to the number of proteins analyzed, the accuracy of spot quantification and reproducibility. In this review article, the efficiency of the most widely used dyes was inves...

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Veröffentlicht in:Materials 2010-10, Vol.3 (10), p.4784-4792
1. Verfasser: Chevalier, François
Format: Artikel
Sprache:eng
Schlagworte:
Biochemistry
Biochemistry, Molecular Biology
Genomics
Life Sciences
Review
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Zusammenfassung:Staining of two-dimensional gels is a primary concern in proteomic studies using two-dimensional gel electrophoresis with respect to the number of proteins analyzed, the accuracy of spot quantification and reproducibility. In this review article, the efficiency of the most widely used dyes was investigated. Visible dyes (Coomassie blue and silver nitrate), fluorescent dyes (Sypro Ruby, Deep Purple) and cyanine labeled methods were compared.
ISSN:1996-1944
1996-1944
DOI:10.3390/ma3104784