Aliphatic Chains of Esterified Lipids in Isolated Eyespots of Euglena gracilis var. bacillaris

Isolated eyespot granules of Euglena gracilis Klebs var. bacillaris Pringsheim contained approximately 6% lipids (based on protein). Separation of the lipid extracts by thin layer chromatography revealed four major fractions: wax esters, triacylglycerols, free fatty acids, and phospholipids. Methano...

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Veröffentlicht in:	Plant Physiol.; (United States) 1976-04, Vol.57 (4), p.645-646
Hauptverfasser:	Lula L. Hilenski, Walne, Patricia L., Snyder, Fred
Format:	Artikel
Sprache:	eng
Schlagworte:	550200 - Biochemistry ACETATES ALCOHOLS ALGAE BASIC BIOLOGICAL SCIENCES BIOMASS BODY CARBOXYLIC ACID SALTS CARBOXYLIC ACIDS CHEMICAL ANALYSIS CHEMICAL REACTIONS CHROMATOGRAPHY ENERGY SOURCES ESTERIFICATION ESTERS EUGLENA EYES Fatty acids Fatty alcohols GLYCEROL HYDROXY COMPOUNDS LIPIDS METHANOL MICROORGANISMS ORGANIC ACIDS ORGANIC COMPOUNDS ORGANIC PHOSPHORUS COMPOUNDS ORGANS PHOSPHOLIPIDS PLANTS PROTEINS QUALITATIVE CHEMICAL ANALYSIS RENEWABLE ENERGY SOURCES SENSE ORGANS SEPARATION PROCESSES Silica gel Solvents THIN-LAYER CHROMATOGRAPHY UNICELLULAR ALGAE Waxes
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Zusammenfassung:	Isolated eyespot granules of Euglena gracilis Klebs var. bacillaris Pringsheim contained approximately 6% lipids (based on protein). Separation of the lipid extracts by thin layer chromatography revealed four major fractions: wax esters, triacylglycerols, free fatty acids, and phospholipids. Methanolysis of each fraction yielded between 27 and 29 different fatty acids ranging from 12:0 to 22:6. Acetates of the fatty alcohols of the wax fraction consisted of 11:0 to 18:0 carbon chains, with 14:0 being the major component; unsaturated alcohols were not detected.
ISSN:	0032-0889 1532-2548
DOI:	10.1104/pp.57.4.645