19F‐NMR Reveals the Role of Mobile Loops in Product and Inhibitor Binding by the São Paulo Metallo‐β‐Lactamase

Resistance to β‐lactam antibiotics mediated by metallo‐β‐lactamases (MBLs) is a growing problem. We describe the use of protein‐observe 19F‐NMR (PrOF NMR) to study the dynamics of the São Paulo MBL (SPM‐1) from β‐lactam‐resistant Pseudomonas aeruginosa. Cysteinyl variants on the α3 and L3 regions, which flank the di‐ZnII active site, were selectively 19F‐labeled using 3‐bromo‐1,1,1‐trifluoroacetone. The PrOF NMR results reveal roles for the mobile α3 and L3 regions in the binding of both inhibitors and hydrolyzed β‐lactam products to SPM‐1. These results have implications for the mechanisms and inhibition of MBLs by β‐lactams and non‐β‐lactams and illustrate the utility of PrOF NMR for efficiently analyzing metal chelation, identifying new binding modes, and studying protein binding from a mixture of equilibrating isomers. Resistance is futile: Metallo‐β‐lactamase (MBL)‐mediated resistance to β‐lactam antibiotics is a growing problem. 19F‐NMR spectroscopy was used to study the dynamics of the São Paulo MBL from β‐lactam‐resistant Pseudomonas aeruginosa. Cysteinyl variants of the α3 and L3 regions were selectively 19F‐labeled using 3‐bromo‐1,1,1‐trifluoroacetone. The results reveal roles for the mobile α3 and L3 regions in the binding of inhibitors and hydrolyzed β‐lactam products.