CCL2 nitration is a negative regulator of chemokine-mediated inflammation

Chemokines promote leukocyte recruitment during inflammation. The oxidative burst is an important effector mechanism, this leads to the generation of reactive nitrogen species (RNS), including peroxynitrite (ONOO). The current study was performed to determine the potential for nitration to alter the...

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Veröffentlicht in:	Scientific reports 2017-03, Vol.7 (1), p.44384-44384, Article 44384
Hauptverfasser:	Barker, Catriona E., Thompson, Sarah, O’Boyle, Graeme, Lortat-Jacob, Hugues, Sheerin, Neil S., Ali, Simi, Kirby, John A.
Format:	Artikel
Sprache:	eng
Schlagworte:	13/106 13/31 14 14/19 38 38/1 631/250/127/98 631/250/256 631/250/98 64 64/60 Animals Binding Sites Biochemistry, Molecular Biology Biological properties Cell Movement - genetics Chemokine CCL2 - chemistry Chemokine CCL2 - metabolism Chemokines Chemokines - metabolism Chemotaxis Heparan sulfate Humanities and Social Sciences Immunofluorescence Inflammation Inflammation - metabolism Inflammation - pathology Intravenous administration Kidneys Leukocyte migration Leukocytes - metabolism Life Sciences Mice Monocyte chemoattractant protein 1 Monocytes - metabolism multidisciplinary Nitration Nitrogen Oxidative Stress - genetics Peroxynitrite Peroxynitrous Acid - chemistry Peroxynitrous Acid - metabolism Reactive nitrogen species Reactive Nitrogen Species - chemistry Reactive Nitrogen Species - metabolism Receptors, CCR2 - genetics Receptors, CCR2 - metabolism Science Spectroscopy Surface plasmon resonance Tyrosine Tyrosine - metabolism
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Zusammenfassung:	Chemokines promote leukocyte recruitment during inflammation. The oxidative burst is an important effector mechanism, this leads to the generation of reactive nitrogen species (RNS), including peroxynitrite (ONOO). The current study was performed to determine the potential for nitration to alter the chemical and biological properties of the prototypical CC chemokine, CCL2. Immunofluorescence was performed to assess the presence of RNS in kidney biopsies. Co-localisation was observed between RNS-modified tyrosine residues and the chemokine CCL2 in diseased kidneys. Nitration reduced the potential of CCL2 to stimulate monocyte migration in diffusion gradient chemotaxis assays (p
ISSN:	2045-2322 2045-2322
DOI:	10.1038/srep44384