Molecular characterization of Myxoboluscatmrigalae (Myxosporea: Myxobolidae) infecting the gill lamellae of carp Cirrhinusmrigala (Hamilton)
The present study attempted sequencing the 18S rRNA gene of Myxobolus catmrigalae infecting the gill lamellae of carp, Cirrhinus mrigala and compared its genetic homology and phylogenetic characteristics with 18S rRNA genes of other Myxobolus spp. The infected fish had up to 3 small, creamy white pl...
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Veröffentlicht in: | Journal of parasitic diseases 2017-03, Vol.41 (1), p.62-70 |
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Sprache: | eng |
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Zusammenfassung: | The present study attempted sequencing the 18S rRNA gene of
Myxobolus
catmrigalae
infecting the gill lamellae of carp,
Cirrhinus
mrigala
and compared its genetic homology and phylogenetic characteristics with 18S rRNA genes of other
Myxobolus
spp. The infected fish had up to 3 small, creamy white plasmodia per gill filament with 30–50 spores each. The spore size was 17.90 ± 0.70 × 7.40 ± 0.40 μm. The sporoplasm contained two large nuclei of size 0.57 ± 0.09 μm and no iodinophilous vacuole. The DNA sequence of
M.
catmrigalae
was clustered phylogenetically with other
Myxobolus
spp. infecting the gills of cyprinids available in GenBank, which showed 77–87 % homogeneity. On the phylogenetic tree,
M.
catmrigalae
(KC933944) was clustered with
M.
pavlovskii
(HM991164) infecting the gill lamellae of silver carp,
Hypophthalmichthys
molitrix
. The species most closely related to
M.
catmrigalae
in GenBank was
M.
pavlovskii
(AF507973) infecting the gill lamellae of big head carp,
Aristichthys
nobilis
with 87 % homogeneity. This is the first report on molecular characterization of gill lamellae infecting
M. catmrigalae. |
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ISSN: | 0971-7196 0975-0703 |
DOI: | 10.1007/s12639-016-0750-0 |