Preparation and Characterization of Three Tilmicosin-loaded Lipid Nanoparticles: Physicochemical Properties and in-vitro Antibacterial Activities
Tilmicosin (TLM) is an important antibiotic in veterinary medicine with low bioavailability and safety. This study aimed to formulate and evaluate physicochemical properties, storage stability after lyophilization, and antibacterial activity of three TLM-loaded lipid nanoparticles (TLM-LNPs) includi...
Gespeichert in:
| Veröffentlicht in: | Iranian journal of pharmaceutical research : IJPR 2016-01, Vol.15 (4), p.663-676 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 676 |
|---|---|
| container_issue | 4 |
| container_start_page | 663 |
| container_title | Iranian journal of pharmaceutical research : IJPR |
| container_volume | 15 |
| creator | Al-Qushawi, Alwan Rassouli, Ali Atyabi, Fatemeh Peighambari, Seyed Mostafa Esfandyari-Manesh, Mehdi Shams, Gholam Reza Yazdani, Azam |
| description | Tilmicosin (TLM) is an important antibiotic in veterinary medicine with low bioavailability and safety. This study aimed to formulate and evaluate physicochemical properties, storage stability after lyophilization, and antibacterial activity of three TLM-loaded lipid nanoparticles (TLM-LNPs) including solid lipid nanoparticles (SLNs), nanostructured lipid carriers (NLCs), and lipid-core nanocapsules (LNCs). Physicochemical parameters such as particle size-mean diameter, polydispersity index, zeta potential, drug encapsulation efficiency (EE), loading capacity, and morphology of the formulations were evaluated and the effects of various cryoprotectants during lyophilization and storage for 8 weeks were also studied. The profiles of TLM release and the antibacterial activities of these TLM-LNPs suspensions (against
and
) were tested in comparison with their corresponding powders. TLM-LNPs suspensions were in nano-scale range with mean diameters of 186.3 ± 1.5, 149.6 ± 3.0, and 85.0 ± 1.0nm, and also EE, 69.1, 86.3, and 94.3% for TLM- SLNs, TLM-NLCs, and TLM- LNCs respectively. TLM-LNCs gave the best results with significantly low particle size and high EE (p |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5316245</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1874792703</sourcerecordid><originalsourceid>FETCH-LOGICAL-p266t-f3cdce0154674e9046ad10ea27e4bc0f3028eae123a0631565ff0ccf9ee05a5c3</originalsourceid><addsrcrecordid>eNpVkctOwzAQRSMEouXxC8hLNpH8iJ2EBVJV8ZIq6KKsI9eZECPXDrZbqfwFf4yhBcHK45kz5y7mIBuTkopcVDU-_KoZzzGj1Sg7CeEVYy7qAh9nI1pRQRiux9nH3MMgvYzaWSRti6Z9-qkIXr_vmq5Di94DoIU2K61c0DY3TrbQopkedIsepXVJEbUyEK7QvN-GhKkeEi0Nmns3QJpC-Pan7Y2O3qGJjXq5S0rUREWd-ok6y446aQKc79_T7Pn2ZjG9z2dPdw_TySwfqBAx75hqFWDCC1EWUONCyJZgkLSEYqlwxzCtQAKhTGLBCBe867BSXQ2AueSKnWbXO--wXq4guWz00jSD1yvpt42Tuvk_sbpvXtym4YwIWvAkuNwLvHtbQ4jNSgcFxkgLbh0aUpVFWdMSs4Re_M36Dfm5A_sE-KKKrA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1874792703</pqid></control><display><type>article</type><title>Preparation and Characterization of Three Tilmicosin-loaded Lipid Nanoparticles: Physicochemical Properties and in-vitro Antibacterial Activities</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Al-Qushawi, Alwan ; Rassouli, Ali ; Atyabi, Fatemeh ; Peighambari, Seyed Mostafa ; Esfandyari-Manesh, Mehdi ; Shams, Gholam Reza ; Yazdani, Azam</creator><creatorcontrib>Al-Qushawi, Alwan ; Rassouli, Ali ; Atyabi, Fatemeh ; Peighambari, Seyed Mostafa ; Esfandyari-Manesh, Mehdi ; Shams, Gholam Reza ; Yazdani, Azam</creatorcontrib><description>Tilmicosin (TLM) is an important antibiotic in veterinary medicine with low bioavailability and safety. This study aimed to formulate and evaluate physicochemical properties, storage stability after lyophilization, and antibacterial activity of three TLM-loaded lipid nanoparticles (TLM-LNPs) including solid lipid nanoparticles (SLNs), nanostructured lipid carriers (NLCs), and lipid-core nanocapsules (LNCs). Physicochemical parameters such as particle size-mean diameter, polydispersity index, zeta potential, drug encapsulation efficiency (EE), loading capacity, and morphology of the formulations were evaluated and the effects of various cryoprotectants during lyophilization and storage for 8 weeks were also studied. The profiles of TLM release and the antibacterial activities of these TLM-LNPs suspensions (against
and
) were tested in comparison with their corresponding powders. TLM-LNPs suspensions were in nano-scale range with mean diameters of 186.3 ± 1.5, 149.6 ± 3.0, and 85.0 ± 1.0nm, and also EE, 69.1, 86.3, and 94.3% for TLM- SLNs, TLM-NLCs, and TLM- LNCs respectively. TLM-LNCs gave the best results with significantly low particle size and high EE (p<0.05). Mannitol was the most effective cryoprotectant for lyophilization and storage of TLM-LNPs. The drug release profiles were biphasic and the release times were longer at pH 7.4 where TLM-NLCs and TLM-LNCs powders showed longer release times. In microbiological tests,
was about 4 times more sensitive than
to TLM-LNPs with minimum inhibitory concentration ranges of 0.5-1.0 and 2-4 µg/mL respectively, and TLM-LNCs exhibited the best antibacterial activities. In conclusion, TLM-LNP formulations especially TLM-LNCs and TLM-NLCs are promising carriers for TLM with better drug encapsulation capacity, release behavior, and antibacterial activity.</description><identifier>ISSN: 1735-0328</identifier><identifier>EISSN: 1726-6890</identifier><identifier>PMID: 28261309</identifier><language>eng</language><publisher>Iran: Shaheed Beheshti University of Medical Sciences</publisher><subject>Original</subject><ispartof>Iranian journal of pharmaceutical research : IJPR, 2016-01, Vol.15 (4), p.663-676</ispartof><rights>2016 by School of Pharmacy Shaheed Beheshti University of Medical Sciences and Health Services</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5316245/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5316245/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28261309$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Al-Qushawi, Alwan</creatorcontrib><creatorcontrib>Rassouli, Ali</creatorcontrib><creatorcontrib>Atyabi, Fatemeh</creatorcontrib><creatorcontrib>Peighambari, Seyed Mostafa</creatorcontrib><creatorcontrib>Esfandyari-Manesh, Mehdi</creatorcontrib><creatorcontrib>Shams, Gholam Reza</creatorcontrib><creatorcontrib>Yazdani, Azam</creatorcontrib><title>Preparation and Characterization of Three Tilmicosin-loaded Lipid Nanoparticles: Physicochemical Properties and in-vitro Antibacterial Activities</title><title>Iranian journal of pharmaceutical research : IJPR</title><addtitle>Iran J Pharm Res</addtitle><description>Tilmicosin (TLM) is an important antibiotic in veterinary medicine with low bioavailability and safety. This study aimed to formulate and evaluate physicochemical properties, storage stability after lyophilization, and antibacterial activity of three TLM-loaded lipid nanoparticles (TLM-LNPs) including solid lipid nanoparticles (SLNs), nanostructured lipid carriers (NLCs), and lipid-core nanocapsules (LNCs). Physicochemical parameters such as particle size-mean diameter, polydispersity index, zeta potential, drug encapsulation efficiency (EE), loading capacity, and morphology of the formulations were evaluated and the effects of various cryoprotectants during lyophilization and storage for 8 weeks were also studied. The profiles of TLM release and the antibacterial activities of these TLM-LNPs suspensions (against
and
) were tested in comparison with their corresponding powders. TLM-LNPs suspensions were in nano-scale range with mean diameters of 186.3 ± 1.5, 149.6 ± 3.0, and 85.0 ± 1.0nm, and also EE, 69.1, 86.3, and 94.3% for TLM- SLNs, TLM-NLCs, and TLM- LNCs respectively. TLM-LNCs gave the best results with significantly low particle size and high EE (p<0.05). Mannitol was the most effective cryoprotectant for lyophilization and storage of TLM-LNPs. The drug release profiles were biphasic and the release times were longer at pH 7.4 where TLM-NLCs and TLM-LNCs powders showed longer release times. In microbiological tests,
was about 4 times more sensitive than
to TLM-LNPs with minimum inhibitory concentration ranges of 0.5-1.0 and 2-4 µg/mL respectively, and TLM-LNCs exhibited the best antibacterial activities. In conclusion, TLM-LNP formulations especially TLM-LNCs and TLM-NLCs are promising carriers for TLM with better drug encapsulation capacity, release behavior, and antibacterial activity.</description><subject>Original</subject><issn>1735-0328</issn><issn>1726-6890</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNpVkctOwzAQRSMEouXxC8hLNpH8iJ2EBVJV8ZIq6KKsI9eZECPXDrZbqfwFf4yhBcHK45kz5y7mIBuTkopcVDU-_KoZzzGj1Sg7CeEVYy7qAh9nI1pRQRiux9nH3MMgvYzaWSRti6Z9-qkIXr_vmq5Di94DoIU2K61c0DY3TrbQopkedIsepXVJEbUyEK7QvN-GhKkeEi0Nmns3QJpC-Pan7Y2O3qGJjXq5S0rUREWd-ok6y446aQKc79_T7Pn2ZjG9z2dPdw_TySwfqBAx75hqFWDCC1EWUONCyJZgkLSEYqlwxzCtQAKhTGLBCBe867BSXQ2AueSKnWbXO--wXq4guWz00jSD1yvpt42Tuvk_sbpvXtym4YwIWvAkuNwLvHtbQ4jNSgcFxkgLbh0aUpVFWdMSs4Re_M36Dfm5A_sE-KKKrA</recordid><startdate>20160101</startdate><enddate>20160101</enddate><creator>Al-Qushawi, Alwan</creator><creator>Rassouli, Ali</creator><creator>Atyabi, Fatemeh</creator><creator>Peighambari, Seyed Mostafa</creator><creator>Esfandyari-Manesh, Mehdi</creator><creator>Shams, Gholam Reza</creator><creator>Yazdani, Azam</creator><general>Shaheed Beheshti University of Medical Sciences</general><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160101</creationdate><title>Preparation and Characterization of Three Tilmicosin-loaded Lipid Nanoparticles: Physicochemical Properties and in-vitro Antibacterial Activities</title><author>Al-Qushawi, Alwan ; Rassouli, Ali ; Atyabi, Fatemeh ; Peighambari, Seyed Mostafa ; Esfandyari-Manesh, Mehdi ; Shams, Gholam Reza ; Yazdani, Azam</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p266t-f3cdce0154674e9046ad10ea27e4bc0f3028eae123a0631565ff0ccf9ee05a5c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Original</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Al-Qushawi, Alwan</creatorcontrib><creatorcontrib>Rassouli, Ali</creatorcontrib><creatorcontrib>Atyabi, Fatemeh</creatorcontrib><creatorcontrib>Peighambari, Seyed Mostafa</creatorcontrib><creatorcontrib>Esfandyari-Manesh, Mehdi</creatorcontrib><creatorcontrib>Shams, Gholam Reza</creatorcontrib><creatorcontrib>Yazdani, Azam</creatorcontrib><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Iranian journal of pharmaceutical research : IJPR</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Al-Qushawi, Alwan</au><au>Rassouli, Ali</au><au>Atyabi, Fatemeh</au><au>Peighambari, Seyed Mostafa</au><au>Esfandyari-Manesh, Mehdi</au><au>Shams, Gholam Reza</au><au>Yazdani, Azam</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation and Characterization of Three Tilmicosin-loaded Lipid Nanoparticles: Physicochemical Properties and in-vitro Antibacterial Activities</atitle><jtitle>Iranian journal of pharmaceutical research : IJPR</jtitle><addtitle>Iran J Pharm Res</addtitle><date>2016-01-01</date><risdate>2016</risdate><volume>15</volume><issue>4</issue><spage>663</spage><epage>676</epage><pages>663-676</pages><issn>1735-0328</issn><eissn>1726-6890</eissn><abstract>Tilmicosin (TLM) is an important antibiotic in veterinary medicine with low bioavailability and safety. This study aimed to formulate and evaluate physicochemical properties, storage stability after lyophilization, and antibacterial activity of three TLM-loaded lipid nanoparticles (TLM-LNPs) including solid lipid nanoparticles (SLNs), nanostructured lipid carriers (NLCs), and lipid-core nanocapsules (LNCs). Physicochemical parameters such as particle size-mean diameter, polydispersity index, zeta potential, drug encapsulation efficiency (EE), loading capacity, and morphology of the formulations were evaluated and the effects of various cryoprotectants during lyophilization and storage for 8 weeks were also studied. The profiles of TLM release and the antibacterial activities of these TLM-LNPs suspensions (against
and
) were tested in comparison with their corresponding powders. TLM-LNPs suspensions were in nano-scale range with mean diameters of 186.3 ± 1.5, 149.6 ± 3.0, and 85.0 ± 1.0nm, and also EE, 69.1, 86.3, and 94.3% for TLM- SLNs, TLM-NLCs, and TLM- LNCs respectively. TLM-LNCs gave the best results with significantly low particle size and high EE (p<0.05). Mannitol was the most effective cryoprotectant for lyophilization and storage of TLM-LNPs. The drug release profiles were biphasic and the release times were longer at pH 7.4 where TLM-NLCs and TLM-LNCs powders showed longer release times. In microbiological tests,
was about 4 times more sensitive than
to TLM-LNPs with minimum inhibitory concentration ranges of 0.5-1.0 and 2-4 µg/mL respectively, and TLM-LNCs exhibited the best antibacterial activities. In conclusion, TLM-LNP formulations especially TLM-LNCs and TLM-NLCs are promising carriers for TLM with better drug encapsulation capacity, release behavior, and antibacterial activity.</abstract><cop>Iran</cop><pub>Shaheed Beheshti University of Medical Sciences</pub><pmid>28261309</pmid><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1735-0328 |
| ispartof | Iranian journal of pharmaceutical research : IJPR, 2016-01, Vol.15 (4), p.663-676 |
| issn | 1735-0328 1726-6890 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5316245 |
| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Original |
| title | Preparation and Characterization of Three Tilmicosin-loaded Lipid Nanoparticles: Physicochemical Properties and in-vitro Antibacterial Activities |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-23T20%3A22%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Preparation%20and%20Characterization%20of%20Three%20Tilmicosin-loaded%20Lipid%20Nanoparticles:%20Physicochemical%20Properties%20and%20in-vitro%20Antibacterial%20Activities&rft.jtitle=Iranian%20journal%20of%20pharmaceutical%20research%20:%20IJPR&rft.au=Al-Qushawi,%20Alwan&rft.date=2016-01-01&rft.volume=15&rft.issue=4&rft.spage=663&rft.epage=676&rft.pages=663-676&rft.issn=1735-0328&rft.eissn=1726-6890&rft_id=info:doi/&rft_dat=%3Cproquest_pubme%3E1874792703%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1874792703&rft_id=info:pmid/28261309&rfr_iscdi=true |