Integrated Omics Analysis of Pathogenic Host Responses during Pandemic H1N1 Influenza Virus Infection: The Crucial Role of Lipid Metabolism

Pandemic influenza viruses modulate proinflammatory responses that can lead to immunopathogenesis. We present an extensive and systematic profiling of lipids, metabolites, and proteins in respiratory compartments of ferrets infected with either 1918 or 2009 human pandemic H1N1 influenza viruses. Integrative analysis of high-throughput omics data with virologic and histopathologic data uncovered relationships between host responses and phenotypic outcomes of viral infection. Proinflammatory lipid precursors in the trachea following 1918 infection correlated with severe tracheal lesions. Using an algorithm to infer cell quantity changes from gene expression data, we found enrichment of distinct T cell subpopulations in the trachea. There was also a predicted increase in inflammatory monocytes in the lung of 1918 virus-infected animals that was sustained throughout infection. This study presents a unique resource to the influenza research community and demonstrates the utility of an integrative systems approach for characterization of lipid metabolism alterations underlying respiratory responses to viruses. [Display omitted] •Conducted lipidomic, metabolomic, and proteomic profiling of virus-infected ferrets•1918 and CA04 viruses produce different histologic lesions and metabolic changes•Integrated omics analysis shows dynamic host responses in respiratory immunity•Proinflammatory lipid precursors correlate with influenza virus pathogenesis Influenza virus induces immune responses that contribute to respiratory disease. Tisoncik-Go et al. integrate multiomics data with histopathologic and virologic phenotypes in respiratory tissues of ferrets infected with either a contemporary or historic pandemic H1N1 influenza virus to uncover a correlation between alterations in lipid metabolism and enhanced inflammation.