High phenolics Rutgers Scarlet Lettuce improves glucose metabolism in high fat diet-induced obese mice
Scope The ability of high phenolic Rutgers Scarlet Lettuce (RSL) to attenuate metabolic syndrome and gut dysbiosis was studied in very high fat diet (VHFD)‐fed mice. Phenolic absorption was assessed in vivo and in a gastrointestinal tract model. Methods and results Mice were fed VHFD, VHFD supplemen...
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| Veröffentlicht in: | Molecular nutrition & food research 2016-11, Vol.60 (11), p.2367-2378 |
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| creator | Cheng, Diana M. Roopchand, Diana E. Poulev, Alexander Kuhn, Peter Armas, Isabel Johnson, William D. Oren, Andrew Ribnicky, David Zelzion, Ehud Bhattacharya, Debashish Raskin, Ilya |
| description | Scope
The ability of high phenolic Rutgers Scarlet Lettuce (RSL) to attenuate metabolic syndrome and gut dysbiosis was studied in very high fat diet (VHFD)‐fed mice. Phenolic absorption was assessed in vivo and in a gastrointestinal tract model.
Methods and results
Mice were fed VHFD, VHFD supplemented with RSL (RSL‐VHFD) or store‐purchased green lettuce (GL‐VHFD), or low‐fat diet (LFD) for 13 weeks. Compared to VHFD or GL‐VHFD‐fed groups, RSL‐VHFD group showed significantly improved oral glucose tolerance (p |
| doi_str_mv | 10.1002/mnfr.201600290 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5240636</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1850775686</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5066-330a6f5e9f702e2b5a858fd0b1c24b3ea0e6430ab1c1bfc317b8c9d53e3b337e3</originalsourceid><addsrcrecordid>eNqNkU1v1DAURSMEoqWwZYm8ZJPB9ovtZIOEWmaKNBSpBXVpOc7LjCEfg-0U-u9xNCWCFazsJ59z9aybZS8ZXTFK-Zt-aP2KUybTUNFH2SmTDPKCATxe7lycZM9C-EopMF7A0-yEK8GroihPs_bS7fbksMdh7JwN5HqKO_SB3FjjO4xkizFOFonrD368w0B23WTHgKTHaOrkhJ64geznlNZE0jiMuRua5DRkrHEmncXn2ZPWdAFfPJxn2Zf1-8_nl_n20-bD-bttbgWVMgegRrYCq1ZRjrwWphRl29CaWV7UgIaiLBKTZla3FpiqS1s1AhBqAIVwlr095h6musfG4hC96fTBu974ez0ap_9-Gdxe78Y7LXhBJcgU8PohwI_fJwxR9y5Y7Doz4DgFzUpBlRKy_B8UhJQlVCqhqyNq_RiCx3bZiFE996jnHvXSYxJe_fmPBf9dXAKKI_DDdXj_jzj98Wp9zRWbd86PmgsRfy6a8d-0VKCEvr3a6Bt2sb7YJO0WfgEsFLpi</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1835668397</pqid></control><display><type>article</type><title>High phenolics Rutgers Scarlet Lettuce improves glucose metabolism in high fat diet-induced obese mice</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Cheng, Diana M. ; Roopchand, Diana E. ; Poulev, Alexander ; Kuhn, Peter ; Armas, Isabel ; Johnson, William D. ; Oren, Andrew ; Ribnicky, David ; Zelzion, Ehud ; Bhattacharya, Debashish ; Raskin, Ilya</creator><creatorcontrib>Cheng, Diana M. ; Roopchand, Diana E. ; Poulev, Alexander ; Kuhn, Peter ; Armas, Isabel ; Johnson, William D. ; Oren, Andrew ; Ribnicky, David ; Zelzion, Ehud ; Bhattacharya, Debashish ; Raskin, Ilya</creatorcontrib><description>Scope
The ability of high phenolic Rutgers Scarlet Lettuce (RSL) to attenuate metabolic syndrome and gut dysbiosis was studied in very high fat diet (VHFD)‐fed mice. Phenolic absorption was assessed in vivo and in a gastrointestinal tract model.
Methods and results
Mice were fed VHFD, VHFD supplemented with RSL (RSL‐VHFD) or store‐purchased green lettuce (GL‐VHFD), or low‐fat diet (LFD) for 13 weeks. Compared to VHFD or GL‐VHFD‐fed groups, RSL‐VHFD group showed significantly improved oral glucose tolerance (p<0.05). Comparison of VHFD, RSL‐VHFD, and GL‐VHFD groups revealed no significant differences with respect to insulin tolerance, hepatic lipids, body weight gain, fat mass, plasma glucose, triglycerides, free fatty acid, and lipopolysaccharide levels, as well as relative abundances of major bacterial phyla from 16S rDNA amplicon data sequences (from fecal and cecal samples). However, RSL and GL‐supplementation increased abundance of several taxa involved in plant polysaccharide degradation/fermentation. RSL phenolics chlorogenic acid, quercetin‐3‐glucoside, and quercetin‐malonyl‐glucoside were bioaccessible in the TIM‐1 digestion model, but had relatively low recovery.
Conclusions
RSL phenolics contributed to attenuation of post‐prandial hyperglycemia. Changes in gut microbiota were likely due to microbiota accessible carbohydrates in RSL and GL rather than RSL phenolics, which may be metabolized, absorbed, or degraded before reaching the colon.
Rutgers Scarlet Lettuce (RSL) phenolics, chlorogenic acid, quercetin malonyl‐glucoside, and cyanidin malonyl‐glucoside, contribute to attenuation of post‐prandial hyperglycemia. Changes in gut microbiota are due to the microbiota accessible carbohydrates in RSL and green lettuce diets rather than RSL phenolics, which are likely metabolized, absorbed, or degraded before reaching the lower intestine.</description><identifier>ISSN: 1613-4125</identifier><identifier>EISSN: 1613-4133</identifier><identifier>DOI: 10.1002/mnfr.201600290</identifier><identifier>PMID: 27529448</identifier><language>eng</language><publisher>Germany: Blackwell Publishing Ltd</publisher><subject>Animals ; Bioaccessibility ; Carbohydrate Metabolism ; Diet, Fat-Restricted ; Diet, High-Fat - adverse effects ; Dietary Fats - metabolism ; Gastrointestinal Tract - microbiology ; Glucose - metabolism ; Glucose Tolerance Test ; Gut microbiota ; Hyperglycemia - metabolism ; Lactuca - chemistry ; Lettuce ; Liver - metabolism ; Male ; Metabolic syndrome ; Metabolic Syndrome - metabolism ; Mice ; Mice, Inbred C57BL ; Mice, Obese ; Obesity - metabolism ; Phenolics ; Quercetin - analogs & derivatives ; Triglycerides - metabolism ; Weight Gain</subject><ispartof>Molecular nutrition & food research, 2016-11, Vol.60 (11), p.2367-2378</ispartof><rights>2016 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5066-330a6f5e9f702e2b5a858fd0b1c24b3ea0e6430ab1c1bfc317b8c9d53e3b337e3</citedby><cites>FETCH-LOGICAL-c5066-330a6f5e9f702e2b5a858fd0b1c24b3ea0e6430ab1c1bfc317b8c9d53e3b337e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fmnfr.201600290$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fmnfr.201600290$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,780,784,885,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27529448$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cheng, Diana M.</creatorcontrib><creatorcontrib>Roopchand, Diana E.</creatorcontrib><creatorcontrib>Poulev, Alexander</creatorcontrib><creatorcontrib>Kuhn, Peter</creatorcontrib><creatorcontrib>Armas, Isabel</creatorcontrib><creatorcontrib>Johnson, William D.</creatorcontrib><creatorcontrib>Oren, Andrew</creatorcontrib><creatorcontrib>Ribnicky, David</creatorcontrib><creatorcontrib>Zelzion, Ehud</creatorcontrib><creatorcontrib>Bhattacharya, Debashish</creatorcontrib><creatorcontrib>Raskin, Ilya</creatorcontrib><title>High phenolics Rutgers Scarlet Lettuce improves glucose metabolism in high fat diet-induced obese mice</title><title>Molecular nutrition & food research</title><addtitle>Mol. Nutr. Food Res</addtitle><description>Scope
The ability of high phenolic Rutgers Scarlet Lettuce (RSL) to attenuate metabolic syndrome and gut dysbiosis was studied in very high fat diet (VHFD)‐fed mice. Phenolic absorption was assessed in vivo and in a gastrointestinal tract model.
Methods and results
Mice were fed VHFD, VHFD supplemented with RSL (RSL‐VHFD) or store‐purchased green lettuce (GL‐VHFD), or low‐fat diet (LFD) for 13 weeks. Compared to VHFD or GL‐VHFD‐fed groups, RSL‐VHFD group showed significantly improved oral glucose tolerance (p<0.05). Comparison of VHFD, RSL‐VHFD, and GL‐VHFD groups revealed no significant differences with respect to insulin tolerance, hepatic lipids, body weight gain, fat mass, plasma glucose, triglycerides, free fatty acid, and lipopolysaccharide levels, as well as relative abundances of major bacterial phyla from 16S rDNA amplicon data sequences (from fecal and cecal samples). However, RSL and GL‐supplementation increased abundance of several taxa involved in plant polysaccharide degradation/fermentation. RSL phenolics chlorogenic acid, quercetin‐3‐glucoside, and quercetin‐malonyl‐glucoside were bioaccessible in the TIM‐1 digestion model, but had relatively low recovery.
Conclusions
RSL phenolics contributed to attenuation of post‐prandial hyperglycemia. Changes in gut microbiota were likely due to microbiota accessible carbohydrates in RSL and GL rather than RSL phenolics, which may be metabolized, absorbed, or degraded before reaching the colon.
Rutgers Scarlet Lettuce (RSL) phenolics, chlorogenic acid, quercetin malonyl‐glucoside, and cyanidin malonyl‐glucoside, contribute to attenuation of post‐prandial hyperglycemia. Changes in gut microbiota are due to the microbiota accessible carbohydrates in RSL and green lettuce diets rather than RSL phenolics, which are likely metabolized, absorbed, or degraded before reaching the lower intestine.</description><subject>Animals</subject><subject>Bioaccessibility</subject><subject>Carbohydrate Metabolism</subject><subject>Diet, Fat-Restricted</subject><subject>Diet, High-Fat - adverse effects</subject><subject>Dietary Fats - metabolism</subject><subject>Gastrointestinal Tract - microbiology</subject><subject>Glucose - metabolism</subject><subject>Glucose Tolerance Test</subject><subject>Gut microbiota</subject><subject>Hyperglycemia - metabolism</subject><subject>Lactuca - chemistry</subject><subject>Lettuce</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Metabolic syndrome</subject><subject>Metabolic Syndrome - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Obese</subject><subject>Obesity - metabolism</subject><subject>Phenolics</subject><subject>Quercetin - analogs & derivatives</subject><subject>Triglycerides - metabolism</subject><subject>Weight Gain</subject><issn>1613-4125</issn><issn>1613-4133</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAURSMEoqWwZYm8ZJPB9ovtZIOEWmaKNBSpBXVpOc7LjCEfg-0U-u9xNCWCFazsJ59z9aybZS8ZXTFK-Zt-aP2KUybTUNFH2SmTDPKCATxe7lycZM9C-EopMF7A0-yEK8GroihPs_bS7fbksMdh7JwN5HqKO_SB3FjjO4xkizFOFonrD368w0B23WTHgKTHaOrkhJ64geznlNZE0jiMuRua5DRkrHEmncXn2ZPWdAFfPJxn2Zf1-8_nl_n20-bD-bttbgWVMgegRrYCq1ZRjrwWphRl29CaWV7UgIaiLBKTZla3FpiqS1s1AhBqAIVwlr095h6musfG4hC96fTBu974ez0ap_9-Gdxe78Y7LXhBJcgU8PohwI_fJwxR9y5Y7Doz4DgFzUpBlRKy_B8UhJQlVCqhqyNq_RiCx3bZiFE996jnHvXSYxJe_fmPBf9dXAKKI_DDdXj_jzj98Wp9zRWbd86PmgsRfy6a8d-0VKCEvr3a6Bt2sb7YJO0WfgEsFLpi</recordid><startdate>201611</startdate><enddate>201611</enddate><creator>Cheng, Diana M.</creator><creator>Roopchand, Diana E.</creator><creator>Poulev, Alexander</creator><creator>Kuhn, Peter</creator><creator>Armas, Isabel</creator><creator>Johnson, William D.</creator><creator>Oren, Andrew</creator><creator>Ribnicky, David</creator><creator>Zelzion, Ehud</creator><creator>Bhattacharya, Debashish</creator><creator>Raskin, Ilya</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>5PM</scope></search><sort><creationdate>201611</creationdate><title>High phenolics Rutgers Scarlet Lettuce improves glucose metabolism in high fat diet-induced obese mice</title><author>Cheng, Diana M. ; Roopchand, Diana E. ; Poulev, Alexander ; Kuhn, Peter ; Armas, Isabel ; Johnson, William D. ; Oren, Andrew ; Ribnicky, David ; Zelzion, Ehud ; Bhattacharya, Debashish ; Raskin, Ilya</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5066-330a6f5e9f702e2b5a858fd0b1c24b3ea0e6430ab1c1bfc317b8c9d53e3b337e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Bioaccessibility</topic><topic>Carbohydrate Metabolism</topic><topic>Diet, Fat-Restricted</topic><topic>Diet, High-Fat - adverse effects</topic><topic>Dietary Fats - metabolism</topic><topic>Gastrointestinal Tract - microbiology</topic><topic>Glucose - metabolism</topic><topic>Glucose Tolerance Test</topic><topic>Gut microbiota</topic><topic>Hyperglycemia - metabolism</topic><topic>Lactuca - chemistry</topic><topic>Lettuce</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Metabolic syndrome</topic><topic>Metabolic Syndrome - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Obese</topic><topic>Obesity - metabolism</topic><topic>Phenolics</topic><topic>Quercetin - analogs & derivatives</topic><topic>Triglycerides - metabolism</topic><topic>Weight Gain</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cheng, Diana M.</creatorcontrib><creatorcontrib>Roopchand, Diana E.</creatorcontrib><creatorcontrib>Poulev, Alexander</creatorcontrib><creatorcontrib>Kuhn, Peter</creatorcontrib><creatorcontrib>Armas, Isabel</creatorcontrib><creatorcontrib>Johnson, William D.</creatorcontrib><creatorcontrib>Oren, Andrew</creatorcontrib><creatorcontrib>Ribnicky, David</creatorcontrib><creatorcontrib>Zelzion, Ehud</creatorcontrib><creatorcontrib>Bhattacharya, Debashish</creatorcontrib><creatorcontrib>Raskin, Ilya</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Molecular nutrition & food research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cheng, Diana M.</au><au>Roopchand, Diana E.</au><au>Poulev, Alexander</au><au>Kuhn, Peter</au><au>Armas, Isabel</au><au>Johnson, William D.</au><au>Oren, Andrew</au><au>Ribnicky, David</au><au>Zelzion, Ehud</au><au>Bhattacharya, Debashish</au><au>Raskin, Ilya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High phenolics Rutgers Scarlet Lettuce improves glucose metabolism in high fat diet-induced obese mice</atitle><jtitle>Molecular nutrition & food research</jtitle><addtitle>Mol. Nutr. Food Res</addtitle><date>2016-11</date><risdate>2016</risdate><volume>60</volume><issue>11</issue><spage>2367</spage><epage>2378</epage><pages>2367-2378</pages><issn>1613-4125</issn><eissn>1613-4133</eissn><abstract>Scope
The ability of high phenolic Rutgers Scarlet Lettuce (RSL) to attenuate metabolic syndrome and gut dysbiosis was studied in very high fat diet (VHFD)‐fed mice. Phenolic absorption was assessed in vivo and in a gastrointestinal tract model.
Methods and results
Mice were fed VHFD, VHFD supplemented with RSL (RSL‐VHFD) or store‐purchased green lettuce (GL‐VHFD), or low‐fat diet (LFD) for 13 weeks. Compared to VHFD or GL‐VHFD‐fed groups, RSL‐VHFD group showed significantly improved oral glucose tolerance (p<0.05). Comparison of VHFD, RSL‐VHFD, and GL‐VHFD groups revealed no significant differences with respect to insulin tolerance, hepatic lipids, body weight gain, fat mass, plasma glucose, triglycerides, free fatty acid, and lipopolysaccharide levels, as well as relative abundances of major bacterial phyla from 16S rDNA amplicon data sequences (from fecal and cecal samples). However, RSL and GL‐supplementation increased abundance of several taxa involved in plant polysaccharide degradation/fermentation. RSL phenolics chlorogenic acid, quercetin‐3‐glucoside, and quercetin‐malonyl‐glucoside were bioaccessible in the TIM‐1 digestion model, but had relatively low recovery.
Conclusions
RSL phenolics contributed to attenuation of post‐prandial hyperglycemia. Changes in gut microbiota were likely due to microbiota accessible carbohydrates in RSL and GL rather than RSL phenolics, which may be metabolized, absorbed, or degraded before reaching the colon.
Rutgers Scarlet Lettuce (RSL) phenolics, chlorogenic acid, quercetin malonyl‐glucoside, and cyanidin malonyl‐glucoside, contribute to attenuation of post‐prandial hyperglycemia. Changes in gut microbiota are due to the microbiota accessible carbohydrates in RSL and green lettuce diets rather than RSL phenolics, which are likely metabolized, absorbed, or degraded before reaching the lower intestine.</abstract><cop>Germany</cop><pub>Blackwell Publishing Ltd</pub><pmid>27529448</pmid><doi>10.1002/mnfr.201600290</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Molecular nutrition & food research, 2016-11, Vol.60 (11), p.2367-2378 |
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| subjects | Animals Bioaccessibility Carbohydrate Metabolism Diet, Fat-Restricted Diet, High-Fat - adverse effects Dietary Fats - metabolism Gastrointestinal Tract - microbiology Glucose - metabolism Glucose Tolerance Test Gut microbiota Hyperglycemia - metabolism Lactuca - chemistry Lettuce Liver - metabolism Male Metabolic syndrome Metabolic Syndrome - metabolism Mice Mice, Inbred C57BL Mice, Obese Obesity - metabolism Phenolics Quercetin - analogs & derivatives Triglycerides - metabolism Weight Gain |
| title | High phenolics Rutgers Scarlet Lettuce improves glucose metabolism in high fat diet-induced obese mice |
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