Force regulated conformational change of integrin αVβ3

Integrins mediate cell adhesion to extracellular matrix and transduce signals bidirectionally across the membrane. Integrin αVβ3 has been shown to play an essential role in tumor metastasis, angiogenesis, hemostasis and phagocytosis. Integrins can take several conformations, including the bent and extended conformations of the ectodomain, which regulate integrin functions. Using a biomembrane force probe, we characterized the bending and unbending conformational changes of single αVβ3 integrins on living cell surfaces in real-time. We measured the probabilities of conformational changes, rates and speeds of conformational transitions, and the dynamic equilibrium between the two conformations, which were regulated by tensile force, dependent on the ligand, and altered by point mutations. These findings provide insights into how αVβ3 acts as a molecular machine and how its physiological function and molecular structure are coupled at the single‐molecule level. •We characterized the bending and unbending conformational changes of a single integrin αVβ3 molecule on a living cell surface in real-time.•The bending and unbending conformational changes are regulated by tensile force, dependent on the ligand, and altered by point mutations.•Our findings provide insights into how the physiological functions and molecular structure of αVβ3 are coupled at the single molecule level.