Nanochannel Electroporation as a Platform for Living Cell Interrogation in Acute Myeloid Leukemia
A living cell interrogation platform based on nanochannel electroporation is demonstrated with analysis of RNAs in single cells. This minimally invasive process is based on individual cells and allows both multi‐target analysis and stimulus‐response analysis by sequential deliveries. The unique plat...
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| Veröffentlicht in: | Advanced science 2015-12, Vol.2 (12), p.1500111-n/a |
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| creator | Zhao, Xi Huang, Xiaomeng Wang, Xinmei Wu, Yun Eisfeld, Ann‐Kathrin Schwind, Sebastian Gallego‐Perez, Daniel Boukany, Pouyan E. Marcucci, Guido I. Lee, Ly James |
| description | A living cell interrogation platform based on nanochannel electroporation is demonstrated with analysis of RNAs in single cells. This minimally invasive process is based on individual cells and allows both multi‐target analysis and stimulus‐response analysis by sequential deliveries. The unique platform possesses a great potential to the comprehensive and lysis‐free nucleic acid analysis on rare or hard‐to‐transfect cells. |
| doi_str_mv | 10.1002/advs.201500111 |
| format | Article |
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KGaA, Weinheim</rights><rights>2015. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). 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This minimally invasive process is based on individual cells and allows both multi‐target analysis and stimulus‐response analysis by sequential deliveries. The unique platform possesses a great potential to the comprehensive and lysis‐free nucleic acid analysis on rare or hard‐to‐transfect cells.</description><subject>Acids</subject><subject>Communication</subject><subject>Communications</subject><subject>Efficiency</subject><subject>fluorescent probes</subject><subject>Gene expression</subject><subject>Genetic engineering</subject><subject>Leukemia</subject><subject>living cell interrogation</subject><subject>MicroRNAs</subject><subject>nanochannel electroporation</subject><subject>Plasmids</subject><subject>Questioning</subject><subject>Regression analysis</subject><subject>RNA recognition</subject><subject>Studies</subject><issn>2198-3844</issn><issn>2198-3844</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkUtvEzEURi1ERavSLUtkiQ2bBF97POPZIEWhQKXQIvHYWn5N6jJjB3smKP8eRymhsOnGtuTjo_v5Q-gFkDkQQt8ou81zSoATAgBP0BmFVsyYqKqnD86n6CLnO1IYzpoKxDN0SptWkBbEGVLXKkRzq0JwPb7snRlT3MSkRh8DVhkr_LlXYxfTgMuCV37rwxovXd_jqzC6lOL6wPqAF2YaHf60c330Fq_c9MMNXj1HJ53qs7u438_Rt_eXX5cfZ6ubD1fLxWpmOKtgJiqwICwwRTTtGl0TbihviWm1MHXndC0E0x1Y7oCB1nVnTQ2iZpUVlumanaO3B-9m0oOzxoUxqV5ukh9U2smovPz3JvhbuY5byaF8DKFF8PpekOLPyeVRDj6bklQFF6csQXBaCyqaPfrqP_QuTimUeJLSljScCEoKNT9QJsWck-uOwwCR-wLlvkB5LLA8ePkwwhH_U1cB2gPwy_du94hOLt59__JX_hv5IKhX</recordid><startdate>201512</startdate><enddate>201512</enddate><creator>Zhao, Xi</creator><creator>Huang, Xiaomeng</creator><creator>Wang, Xinmei</creator><creator>Wu, Yun</creator><creator>Eisfeld, Ann‐Kathrin</creator><creator>Schwind, Sebastian</creator><creator>Gallego‐Perez, Daniel</creator><creator>Boukany, Pouyan E.</creator><creator>Marcucci, Guido I.</creator><creator>Lee, Ly James</creator><general>John Wiley & Sons, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7XB</scope><scope>88I</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>M2O</scope><scope>M2P</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201512</creationdate><title>Nanochannel Electroporation as a Platform for Living Cell Interrogation in Acute Myeloid Leukemia</title><author>Zhao, Xi ; 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| source | Wiley Online Library Open Access; DOAJ Directory of Open Access Journals; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Acids Communication Communications Efficiency fluorescent probes Gene expression Genetic engineering Leukemia living cell interrogation MicroRNAs nanochannel electroporation Plasmids Questioning Regression analysis RNA recognition Studies |
| title | Nanochannel Electroporation as a Platform for Living Cell Interrogation in Acute Myeloid Leukemia |
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