Nanochannel Electroporation as a Platform for Living Cell Interrogation in Acute Myeloid Leukemia

Nanochannel Electroporation as a Platform for Living Cell Interrogation in Acute Myeloid Leukemia

A living cell interrogation platform based on nanochannel electroporation is demonstrated with analysis of RNAs in single cells. This minimally invasive process is based on individual cells and allows both multi‐target analysis and stimulus‐response analysis by sequential deliveries. The unique plat...

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Veröffentlicht in:Advanced science 2015-12, Vol.2 (12), p.1500111-n/a
Hauptverfasser: Zhao, Xi, Huang, Xiaomeng, Wang, Xinmei, Wu, Yun, Eisfeld, Ann‐Kathrin, Schwind, Sebastian, Gallego‐Perez, Daniel, Boukany, Pouyan E., Marcucci, Guido I., Lee, Ly James
Format: Artikel
Sprache:eng
Schlagworte:
Acids
Communication
Communications
Efficiency
fluorescent probes
Gene expression
Genetic engineering
Leukemia
living cell interrogation
MicroRNAs
nanochannel electroporation
Plasmids
Questioning
Regression analysis
RNA recognition
Studies
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Zusammenfassung:A living cell interrogation platform based on nanochannel electroporation is demonstrated with analysis of RNAs in single cells. This minimally invasive process is based on individual cells and allows both multi‐target analysis and stimulus‐response analysis by sequential deliveries. The unique platform possesses a great potential to the comprehensive and lysis‐free nucleic acid analysis on rare or hard‐to‐transfect cells.
ISSN:2198-3844
2198-3844
DOI:10.1002/advs.201500111