Basic methods for fission yeast

The fission yeast Schizosaccharomyces pombe is a popular model system, and has been particularly influential in studies of the cell cycle and chromosome dynamics. Despite its differences from Saccharomyces cerevisiae, the tools and methods for fission yeast are conceptually similar to those used in...

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Veröffentlicht in:	Yeast (Chichester, England) England), 2006-02, Vol.23 (3), p.173-183
Hauptverfasser:	Forsburg, Susan L, Rhind, Nicholas
Format:	Artikel
Sprache:	eng
Schlagworte:	Crosses, Genetic genetics microscopy Molecular Biology - methods Mycology - methods Saccharomyces cerevisiae Schizosaccharomyces - genetics Schizosaccharomyces - growth & development Schizosaccharomyces pombe Schizosaccharomyces pombe Proteins - genetics Schizosaccharomyces pombe Proteins - metabolism Synchrony transformation Transformation, Genetic
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Beschreibung
Zusammenfassung:	The fission yeast Schizosaccharomyces pombe is a popular model system, and has been particularly influential in studies of the cell cycle and chromosome dynamics. Despite its differences from Saccharomyces cerevisiae, the tools and methods for fission yeast are conceptually similar to those used in budding yeast. Here, we present basic methods sufficient for a beginner in this system to carry out most required manipulations for genetic analysis or molecular biology. Copyright © 2006 John Wiley & Sons, Ltd.
ISSN:	0749-503X 1097-0061
DOI:	10.1002/yea.1347