Comparing isogenic strains of Beijing genotype Mycobacterium tuberculosis after acquisition of Isoniazid resistance: A proteomics approach

We determined differences in the protein abundance among two isogenic strains of Mycobacterium tuberculosis (Mtb) with different Isoniazid (INH) susceptibility profiles. The strains were isolated from a pulmonary tuberculosis patient before and after drug treatment. LC‐MS/MS analysis identified 46 M...

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Veröffentlicht in:	Proteomics (Weinheim) 2016-05, Vol.16 (9), p.1376-1380
Hauptverfasser:	Nieto R, Luisa María, Mehaffy, Carolina, Dobos, Karen M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acquisitions & mergers Antitubercular Agents - pharmacology Bacterial Proteins - genetics Bacterial Proteins - metabolism Catalase - genetics Catalase - metabolism Dataset Brief Drug Resistance, Bacterial - genetics Gene Expression Profiling Gene Expression Regulation, Bacterial - drug effects Gene Ontology Genotype Genotype & phenotype Humans Isogenic strains Isoniazid Isoniazid - pharmacology Microbial Sensitivity Tests Microbiology Molecular Sequence Annotation Mutation Mycobacterium tuberculosis Mycobacterium tuberculosis - drug effects Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - isolation & purification Mycobacterium tuberculosis - metabolism Proteomics Resistance Tuberculosis Tuberculosis, Pulmonary - microbiology
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Zusammenfassung:	We determined differences in the protein abundance among two isogenic strains of Mycobacterium tuberculosis (Mtb) with different Isoniazid (INH) susceptibility profiles. The strains were isolated from a pulmonary tuberculosis patient before and after drug treatment. LC‐MS/MS analysis identified 46 Mtb proteins with altered abundance after INH resistance acquisition. Protein abundance comparisons were done evaluating the different bacterial cellular fractions (membrane, cytosol, cell wall and secreted proteins). MS data have been deposited to the ProteomeXchange with identifier PXD002986.
ISSN:	1615-9853 1615-9861
DOI:	10.1002/pmic.201500403