Next generation sequencing data of a defined microbial mock community

Generating sequence data of a defined community composed of organisms with complete reference genomes is indispensable for the benchmarking of new genome sequence analysis methods, including assembly and binning tools. Moreover the validation of new sequencing library protocols and platforms to assess critical components such as sequencing errors and biases relies on such datasets. We here report the next generation metagenomic sequence data of a defined mock community ( M ock B acteria AR chaea C ommunity; MBARC-26), composed of 23 bacterial and 3 archaeal strains with finished genomes. These strains span 10 phyla and 14 classes, a range of GC contents, genome sizes, repeat content and encompass a diverse abundance profile. Short read Illumina and long-read PacBio SMRT sequences of this mock community are described. These data represent a valuable resource for the scientific community, enabling extensive benchmarking and comparative evaluation of bioinformatics tools without the need to simulate data. As such, these data can aid in improving our current sequence data analysis toolkit and spur interest in the development of new tools. Design Type(s) protocol optimization design Measurement Type(s) metagenomics analysis Technology Type(s) DNA sequencing Factor Type(s) Technology Platform Sample Characteristic(s) Clostridium perfringens • Clostridium thermocellum • Coraliomargarita akajimensis • Corynebacterium glutamicum • Desulfosporosinus acidiphilus • Desulfosporosinus meridiei • Desulfotomaculum gibsoniae • Echinicola vietnamensis • Escherichia coli • Fervidobacterium pennivorans • Frateuria aurantia • Halovivax ruber • Hirschia baltica • Meiothermus silvanus • Natronobacterium gregoryi • Natronococcus occultus • Nocardiopsis dassonvillei • Olsenella uli • Pseudomonas stutzeri • Salmonella bongori • Salmonella enterica • Segniliparus rotundus • Spirochaeta smaragdinae • Streptococcus pyogenes • Terriglobus roseus • Thermobacillus composti Machine-accessible metadata file describing the reported data (ISA-Tab format)