Matrix Metalloproteinase 20 Co-expression With Dentin Sialophosphoprotein in Human and Monkey Kidneys
We recently reported the expression of matrix metalloproteinase 20 (MMP20), hitherto thought to be tooth specific, in the metabolically active ductal epithelial cells of human salivary glands. Furthermore, our report indicated that MMP20 co-expressed and potentially interacts with dentin sialophosph...
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description | We recently reported the expression of matrix metalloproteinase 20 (MMP20), hitherto thought to be tooth specific, in the metabolically active ductal epithelial cells of human salivary glands. Furthermore, our report indicated that MMP20 co-expressed and potentially interacts with dentin sialophosphoprotein (DSPP), a member of the small integrin-binding ligand N-linked glycoproteins (SIBLINGs). Our earlier reports have shown the co-expression of three MMPs, MMP2, MMP3, and MMP9, with specific members of the SIBLING family: bone sialoprotein, osteopontin, and dentin matrix protein 1, respectively. This study investigated the expression of MMP20 and verified its co-expression with DSPP in human and monkey kidney sections and human mixed renal cells by IHC, in situ proximity ligation assay, and immunofluorescence. Our results show that MMP20 is expressed in all segments of the human and monkey nephron with marked intensity in the proximal and distal tubules, and was absent in the glomeruli. Furthermore, MMP20 co-expressed with DSPP in the proximal, distal, and collecting tubules, and in mixed renal cells. Consistent with other SIBLING–MMP pairs, the DSPP–MMP20 pair may play a role in the normal turnover of cell surface proteins and/or repair of pericellular matrix proteins of the basement membranes in the metabolically active duct epithelial system of the nephrons. |
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Furthermore, our report indicated that MMP20 co-expressed and potentially interacts with dentin sialophosphoprotein (DSPP), a member of the small integrin-binding ligand N-linked glycoproteins (SIBLINGs). Our earlier reports have shown the co-expression of three MMPs, MMP2, MMP3, and MMP9, with specific members of the SIBLING family: bone sialoprotein, osteopontin, and dentin matrix protein 1, respectively. This study investigated the expression of MMP20 and verified its co-expression with DSPP in human and monkey kidney sections and human mixed renal cells by IHC, in situ proximity ligation assay, and immunofluorescence. Our results show that MMP20 is expressed in all segments of the human and monkey nephron with marked intensity in the proximal and distal tubules, and was absent in the glomeruli. Furthermore, MMP20 co-expressed with DSPP in the proximal, distal, and collecting tubules, and in mixed renal cells. Consistent with other SIBLING–MMP pairs, the DSPP–MMP20 pair may play a role in the normal turnover of cell surface proteins and/or repair of pericellular matrix proteins of the basement membranes in the metabolically active duct epithelial system of the nephrons.</description><identifier>ISSN: 0022-1554</identifier><identifier>EISSN: 1551-5044</identifier><identifier>DOI: 10.1369/0022155416665098</identifier><identifier>PMID: 27666430</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Animals ; Cell Line ; Extracellular Matrix Proteins - metabolism ; Humans ; Kidney - metabolism ; Macaca fascicularis ; Matrix Metalloproteinase 20 - metabolism ; Nephrons - metabolism ; Phosphoproteins - metabolism ; Sialoglycoproteins - metabolism ; Species Specificity</subject><ispartof>The journal of histochemistry and cytochemistry, 2016-10, Vol.64 (10), p.623-636</ispartof><rights>2016 The Histochemical Society</rights><rights>2016 The Histochemical Society.</rights><rights>2016 The Histochemical Society 2016 The Histochemical Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c467t-d62d3fa636bc0b503e0119fd35597099f2172e48c199173abeb193961970f2353</citedby><cites>FETCH-LOGICAL-c467t-d62d3fa636bc0b503e0119fd35597099f2172e48c199173abeb193961970f2353</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1369/0022155416665098$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1369/0022155416665098$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>230,314,776,780,881,21798,27901,27902,43597,43598</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27666430$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ogbureke, Kalu U.E.</creatorcontrib><creatorcontrib>Koli, Komal</creatorcontrib><creatorcontrib>Saxena, Geetu</creatorcontrib><title>Matrix Metalloproteinase 20 Co-expression With Dentin Sialophosphoprotein in Human and Monkey Kidneys</title><title>The journal of histochemistry and cytochemistry</title><addtitle>J Histochem Cytochem</addtitle><description>We recently reported the expression of matrix metalloproteinase 20 (MMP20), hitherto thought to be tooth specific, in the metabolically active ductal epithelial cells of human salivary glands. Furthermore, our report indicated that MMP20 co-expressed and potentially interacts with dentin sialophosphoprotein (DSPP), a member of the small integrin-binding ligand N-linked glycoproteins (SIBLINGs). Our earlier reports have shown the co-expression of three MMPs, MMP2, MMP3, and MMP9, with specific members of the SIBLING family: bone sialoprotein, osteopontin, and dentin matrix protein 1, respectively. This study investigated the expression of MMP20 and verified its co-expression with DSPP in human and monkey kidney sections and human mixed renal cells by IHC, in situ proximity ligation assay, and immunofluorescence. Our results show that MMP20 is expressed in all segments of the human and monkey nephron with marked intensity in the proximal and distal tubules, and was absent in the glomeruli. Furthermore, MMP20 co-expressed with DSPP in the proximal, distal, and collecting tubules, and in mixed renal cells. Consistent with other SIBLING–MMP pairs, the DSPP–MMP20 pair may play a role in the normal turnover of cell surface proteins and/or repair of pericellular matrix proteins of the basement membranes in the metabolically active duct epithelial system of the nephrons.</description><subject>Animals</subject><subject>Cell Line</subject><subject>Extracellular Matrix Proteins - metabolism</subject><subject>Humans</subject><subject>Kidney - metabolism</subject><subject>Macaca fascicularis</subject><subject>Matrix Metalloproteinase 20 - metabolism</subject><subject>Nephrons - metabolism</subject><subject>Phosphoproteins - metabolism</subject><subject>Sialoglycoproteins - metabolism</subject><subject>Species Specificity</subject><issn>0022-1554</issn><issn>1551-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc9rFDEUx4NY7Fq9e5IcvYy-_JzJRZCtWrGLBxWPITPzpps6m6zJjHT_-2bZtVihICSE8P18v-8lj5AXDF4zoc0bAM6ZUpJprRWY5hFZlCurFEj5mCz2crXXT8nTnK8BmJSqeUJOeV0MUsCC4MpNyd_QFU5uHOM2xQl9cBkpB7qMFd5sE-bsY6A__LSm5xgmH-hX7wq8jrnso4eWdTFvXKAu9HQVw0_c0c--D7jLz8jJ4MaMz4_nGfn-4f235UV1-eXjp-W7y6qTup6qXvNeDE4L3XbQKhAIjJmhF0qZGowZOKs5yqZjxrBauBZbZoTRrKgDF0qckbeH3O3cbrDvSrPJjXab_MalnY3O2_tK8Gt7FX_bUqtWwErAq2NAir9mzJPd-NzhOLqAcc6WNbxujOFS_w8qDDTQmILCAe1SzDnhcNcRA7sfpP13kMXy8u-X3Bn-TK4A1QHI7grtdZxTKD_7cOAtss6luA</recordid><startdate>20161001</startdate><enddate>20161001</enddate><creator>Ogbureke, Kalu U.E.</creator><creator>Koli, Komal</creator><creator>Saxena, Geetu</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QP</scope><scope>5PM</scope></search><sort><creationdate>20161001</creationdate><title>Matrix Metalloproteinase 20 Co-expression With Dentin Sialophosphoprotein in Human and Monkey Kidneys</title><author>Ogbureke, Kalu U.E. ; Koli, Komal ; Saxena, Geetu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c467t-d62d3fa636bc0b503e0119fd35597099f2172e48c199173abeb193961970f2353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Cell Line</topic><topic>Extracellular Matrix Proteins - metabolism</topic><topic>Humans</topic><topic>Kidney - metabolism</topic><topic>Macaca fascicularis</topic><topic>Matrix Metalloproteinase 20 - metabolism</topic><topic>Nephrons - metabolism</topic><topic>Phosphoproteins - metabolism</topic><topic>Sialoglycoproteins - metabolism</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ogbureke, Kalu U.E.</creatorcontrib><creatorcontrib>Koli, Komal</creatorcontrib><creatorcontrib>Saxena, Geetu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The journal of histochemistry and cytochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ogbureke, Kalu U.E.</au><au>Koli, Komal</au><au>Saxena, Geetu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Matrix Metalloproteinase 20 Co-expression With Dentin Sialophosphoprotein in Human and Monkey Kidneys</atitle><jtitle>The journal of histochemistry and cytochemistry</jtitle><addtitle>J Histochem Cytochem</addtitle><date>2016-10-01</date><risdate>2016</risdate><volume>64</volume><issue>10</issue><spage>623</spage><epage>636</epage><pages>623-636</pages><issn>0022-1554</issn><eissn>1551-5044</eissn><abstract>We recently reported the expression of matrix metalloproteinase 20 (MMP20), hitherto thought to be tooth specific, in the metabolically active ductal epithelial cells of human salivary glands. Furthermore, our report indicated that MMP20 co-expressed and potentially interacts with dentin sialophosphoprotein (DSPP), a member of the small integrin-binding ligand N-linked glycoproteins (SIBLINGs). Our earlier reports have shown the co-expression of three MMPs, MMP2, MMP3, and MMP9, with specific members of the SIBLING family: bone sialoprotein, osteopontin, and dentin matrix protein 1, respectively. This study investigated the expression of MMP20 and verified its co-expression with DSPP in human and monkey kidney sections and human mixed renal cells by IHC, in situ proximity ligation assay, and immunofluorescence. Our results show that MMP20 is expressed in all segments of the human and monkey nephron with marked intensity in the proximal and distal tubules, and was absent in the glomeruli. Furthermore, MMP20 co-expressed with DSPP in the proximal, distal, and collecting tubules, and in mixed renal cells. Consistent with other SIBLING–MMP pairs, the DSPP–MMP20 pair may play a role in the normal turnover of cell surface proteins and/or repair of pericellular matrix proteins of the basement membranes in the metabolically active duct epithelial system of the nephrons.</abstract><cop>Los Angeles, CA</cop><pub>SAGE Publications</pub><pmid>27666430</pmid><doi>10.1369/0022155416665098</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals Cell Line Extracellular Matrix Proteins - metabolism Humans Kidney - metabolism Macaca fascicularis Matrix Metalloproteinase 20 - metabolism Nephrons - metabolism Phosphoproteins - metabolism Sialoglycoproteins - metabolism Species Specificity |
title | Matrix Metalloproteinase 20 Co-expression With Dentin Sialophosphoprotein in Human and Monkey Kidneys |
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