Externally Applied Static Magnetic Field Enhances Cardiac Retention and Functional Benefit of Magnetically Iron‐Labeled Adipose‐Derived Stem Cells in Infarcted Hearts
Although adipose‐derived stem cells (ASCs) hold the promise of effective therapy for myocardial infarction, low cardiac retention of implanted ASCs has hindered their therapeutic efficiency. We investigated whether an externally applied static magnetic field (SMF) enhances cardiac localization of &q...
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| creator | Wang, Jian Xiang, Bo Deng, Jixian Lin, Hung-Yu Zheng, Dayang Freed, Darren H. Arora, Rakesh C. Tian, Ganghong |
| description | Although adipose‐derived stem cells (ASCs) hold the promise of effective therapy for myocardial infarction, low cardiac retention of implanted ASCs has hindered their therapeutic efficiency. We investigated whether an externally applied static magnetic field (SMF) enhances cardiac localization of "magnetic" cells and promotes heart function recovery when ASCs are preloaded with superparamagnetic iron oxide (SPIO) nanoparticles. The influence of SMF (0.1 Tesla) on the biological activities of SPIO‐labeled ASCs (SPIOASCs) was investigated first. Fifty‐six female rats with myocardial infarction underwent intramyocardial injection of cell culture medium (CCM) or male SPIOASCs with or without the subcutaneous implantable magnet (CCM‐magnet or SPIOASC‐magnet). Four weeks later, endothelial differentiation, angiogenic cytokine secretion, angiogenesis, cardiomyocyte apoptosis, cell retention, and cardiac performance were examined. The 0.1‐Tsela SMF did not adversely affect the viability, proliferation, angiogenic cytokine secretion, and DNA integrity of SPIOASCs. The implanted SPIOASCs could differentiate into endothelial cell, incorporate into newly formed vessels, and secrete multiple angiogenic cytokines. Four weeks after cell transplantation, the number of cardiac SPIOASCs was significantly increased, vascular density was markedly enlarged, fewer apoptotic cardiomyocytes were present, and heart contractile function was substantially improved in the SPIOASC‐magnet treated rats in comparison with the SPIOASC‐treated rats. The SPIOASCs could differentiate into endothelial cells, incorporate into vessels, promote angiogenesis, and inhibit ischemic cardiomyocyte apoptosis. An externally applied SMF offered a secure environment for biological properties of SPIOASCs, increased the cardiac retention of implanted magnetic SPIOASCs, and further enhanced heart function recovery after myocardial infarction.
Significance
This pilot proof‐of‐concept study suggests that a 0.1‐Tesla static magnetic field does not adversely affect the viability, proliferation, angiogenic cytokine secretion, or DNA integrity of the superparamagnetic iron oxide‐labeled adipose‐derived stem cells (SPIOASCs). Implantation of adipose‐derived stem cells promotes myocardial neovascularization and inhibits ischemic cardiomyocyte apoptosis through endothelial differentiation, incorporation into vessels, and paracrine factor secretion. An externally applied static magnetic field enhanced myocardial rete |
| doi_str_mv | 10.5966/sctm.2015-0220 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_24P</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5031175</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1826717868</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4700-8886bd9f80210297474605933998eb07900d04762797813c807421d5bae6e4753</originalsourceid><addsrcrecordid>eNqFUsGO0zAQjRCIXS175YgsceHSMnYS2xESUiktW6kIiV3OluNMdr1ynGKnC73xCXwHn8WX4LRLBVzwxTPj5zfzNC_LnlKYlhXnL6MZuikDWk6AMXiQnTJaigkvJTw8xrw4yc5jvIV0eMUrBo-zEyYKAFGJ0-zH4uuAwWvndmS22TiLDbkc9GANea-vPY7B0qJryMLfaG8wkrkOjdWGfMQB_WB7T7RvyHLrzZhoR96gx9YOpG-PHHv-Vej9z2_f17pGl9rMGrvpI6bKWwz2bt8YOzJH5yKxnqx8q4MZUv0CdRjik-xRq13E8_v7LPu0XFzNLybrD-9W89l6YgoBMJFS8rqpWgmMAqtEIQoOZZXnVSWxTqoBGigEZ0m_pLmRIApGm7LWyLEQZX6WvT7wbrZ1h41JIoN2ahNsp8NO9dqqv1-8vVHX_Z0qIad0T_DiniD0n7cYB9XZaJIs7bHfRkUl44IKyWWCPv8Hettvx3VExVgFNC9KxhJqekCZ0McYsD0OQ0GNTlCjE9ToBDU6IX149qeEI_z33hPg1QHwxTrc_YdOXc6v8pTxkqaJJOS_ANURw2Q</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2290134522</pqid></control><display><type>article</type><title>Externally Applied Static Magnetic Field Enhances Cardiac Retention and Functional Benefit of Magnetically Iron‐Labeled Adipose‐Derived Stem Cells in Infarcted Hearts</title><source>Wiley Online Library Open Access</source><creator>Wang, Jian ; Xiang, Bo ; Deng, Jixian ; Lin, Hung-Yu ; Zheng, Dayang ; Freed, Darren H. ; Arora, Rakesh C. ; Tian, Ganghong</creator><creatorcontrib>Wang, Jian ; Xiang, Bo ; Deng, Jixian ; Lin, Hung-Yu ; Zheng, Dayang ; Freed, Darren H. ; Arora, Rakesh C. ; Tian, Ganghong</creatorcontrib><description>Although adipose‐derived stem cells (ASCs) hold the promise of effective therapy for myocardial infarction, low cardiac retention of implanted ASCs has hindered their therapeutic efficiency. We investigated whether an externally applied static magnetic field (SMF) enhances cardiac localization of "magnetic" cells and promotes heart function recovery when ASCs are preloaded with superparamagnetic iron oxide (SPIO) nanoparticles. The influence of SMF (0.1 Tesla) on the biological activities of SPIO‐labeled ASCs (SPIOASCs) was investigated first. Fifty‐six female rats with myocardial infarction underwent intramyocardial injection of cell culture medium (CCM) or male SPIOASCs with or without the subcutaneous implantable magnet (CCM‐magnet or SPIOASC‐magnet). Four weeks later, endothelial differentiation, angiogenic cytokine secretion, angiogenesis, cardiomyocyte apoptosis, cell retention, and cardiac performance were examined. The 0.1‐Tsela SMF did not adversely affect the viability, proliferation, angiogenic cytokine secretion, and DNA integrity of SPIOASCs. The implanted SPIOASCs could differentiate into endothelial cell, incorporate into newly formed vessels, and secrete multiple angiogenic cytokines. Four weeks after cell transplantation, the number of cardiac SPIOASCs was significantly increased, vascular density was markedly enlarged, fewer apoptotic cardiomyocytes were present, and heart contractile function was substantially improved in the SPIOASC‐magnet treated rats in comparison with the SPIOASC‐treated rats. The SPIOASCs could differentiate into endothelial cells, incorporate into vessels, promote angiogenesis, and inhibit ischemic cardiomyocyte apoptosis. An externally applied SMF offered a secure environment for biological properties of SPIOASCs, increased the cardiac retention of implanted magnetic SPIOASCs, and further enhanced heart function recovery after myocardial infarction.
Significance
This pilot proof‐of‐concept study suggests that a 0.1‐Tesla static magnetic field does not adversely affect the viability, proliferation, angiogenic cytokine secretion, or DNA integrity of the superparamagnetic iron oxide‐labeled adipose‐derived stem cells (SPIOASCs). Implantation of adipose‐derived stem cells promotes myocardial neovascularization and inhibits ischemic cardiomyocyte apoptosis through endothelial differentiation, incorporation into vessels, and paracrine factor secretion. An externally applied static magnetic field enhanced myocardial retention of intramyocardially injected "magnetic" SPIOASCs and promoted cardiac function recovery after myocardial infarction. With further preclinical optimization, this approach may improve the outcome of current stem cell therapy for ischemic myocardial infarction.
Although adipose‐derived stem cells (ASCs) hold the promise of effective therapy for myocardial infarction (MI), low cardiac retention of implanted ASCs has hindered their therapeutic efficiency. We investigated whether an externally applied static magnetic field (SMF) enhanced cardiac localization of “magnetic” ASCs preloaded with superparamagnetic iron oxide (SPIO) nanoparticles and further improved heart function recovery. In conclusion, the SMF increased the cardiac retention of implanted “magnetic” SPIO‐labeled ASCs and enhanced heart function recovery after MI.</description><identifier>ISSN: 2157-6564</identifier><identifier>EISSN: 2157-6580</identifier><identifier>DOI: 10.5966/sctm.2015-0220</identifier><identifier>PMID: 27400797</identifier><language>eng</language><publisher>Durham, NC, USA: AlphaMed Press</publisher><subject>Adipose Tissue - cytology ; Adipose-derived stem cells ; Administrative support ; Angiogenesis ; Animals ; Apoptosis ; Cardiac function ; Cardiomyocytes ; Cell culture ; Cell Differentiation ; Cell Movement ; Cell retention ; Cytokines ; Data analysis ; Deoxyribonucleic acid ; Disease Models, Animal ; DNA ; Drug dosages ; Endothelial cells ; FDA approval ; Female ; Ferric Compounds - pharmacology ; Flow Cytometry ; Heart ; Iron oxides ; Ischemia ; Localization ; Lungs ; Magnetic Fields ; Male ; Metal Nanoparticles ; Muscle contraction ; Myocardial Infarction ; Nanoparticles ; Paracrine signalling ; Pilot Projects ; Rats ; Rats, Transgenic ; Retention ; Science ; Static magnetic field ; Stem Cell Transplantation - methods ; Stem Cells ; Studies ; Superparamagnetic iron oxide ; Tissue Engineering and Regenerative Medicine ; Vascularization</subject><ispartof>Stem cells translational medicine, 2016-10, Vol.5 (10), p.1380-1393</ispartof><rights>2016 AlphaMed Press</rights><rights>AlphaMed Press.</rights><rights>2016. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>AlphaMed Press 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4700-8886bd9f80210297474605933998eb07900d04762797813c807421d5bae6e4753</citedby><cites>FETCH-LOGICAL-c4700-8886bd9f80210297474605933998eb07900d04762797813c807421d5bae6e4753</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5031175/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5031175/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,864,885,1417,11562,27924,27925,45574,45575,46052,46476,53791,53793</link.rule.ids><linktorsrc>$$Uhttps://onlinelibrary.wiley.com/doi/abs/10.5966%2Fsctm.2015-0220$$EView_record_in_Wiley-Blackwell$$FView_record_in_$$GWiley-Blackwell</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27400797$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Jian</creatorcontrib><creatorcontrib>Xiang, Bo</creatorcontrib><creatorcontrib>Deng, Jixian</creatorcontrib><creatorcontrib>Lin, Hung-Yu</creatorcontrib><creatorcontrib>Zheng, Dayang</creatorcontrib><creatorcontrib>Freed, Darren H.</creatorcontrib><creatorcontrib>Arora, Rakesh C.</creatorcontrib><creatorcontrib>Tian, Ganghong</creatorcontrib><title>Externally Applied Static Magnetic Field Enhances Cardiac Retention and Functional Benefit of Magnetically Iron‐Labeled Adipose‐Derived Stem Cells in Infarcted Hearts</title><title>Stem cells translational medicine</title><addtitle>Stem Cells Transl Med</addtitle><description>Although adipose‐derived stem cells (ASCs) hold the promise of effective therapy for myocardial infarction, low cardiac retention of implanted ASCs has hindered their therapeutic efficiency. We investigated whether an externally applied static magnetic field (SMF) enhances cardiac localization of "magnetic" cells and promotes heart function recovery when ASCs are preloaded with superparamagnetic iron oxide (SPIO) nanoparticles. The influence of SMF (0.1 Tesla) on the biological activities of SPIO‐labeled ASCs (SPIOASCs) was investigated first. Fifty‐six female rats with myocardial infarction underwent intramyocardial injection of cell culture medium (CCM) or male SPIOASCs with or without the subcutaneous implantable magnet (CCM‐magnet or SPIOASC‐magnet). Four weeks later, endothelial differentiation, angiogenic cytokine secretion, angiogenesis, cardiomyocyte apoptosis, cell retention, and cardiac performance were examined. The 0.1‐Tsela SMF did not adversely affect the viability, proliferation, angiogenic cytokine secretion, and DNA integrity of SPIOASCs. The implanted SPIOASCs could differentiate into endothelial cell, incorporate into newly formed vessels, and secrete multiple angiogenic cytokines. Four weeks after cell transplantation, the number of cardiac SPIOASCs was significantly increased, vascular density was markedly enlarged, fewer apoptotic cardiomyocytes were present, and heart contractile function was substantially improved in the SPIOASC‐magnet treated rats in comparison with the SPIOASC‐treated rats. The SPIOASCs could differentiate into endothelial cells, incorporate into vessels, promote angiogenesis, and inhibit ischemic cardiomyocyte apoptosis. An externally applied SMF offered a secure environment for biological properties of SPIOASCs, increased the cardiac retention of implanted magnetic SPIOASCs, and further enhanced heart function recovery after myocardial infarction.
Significance
This pilot proof‐of‐concept study suggests that a 0.1‐Tesla static magnetic field does not adversely affect the viability, proliferation, angiogenic cytokine secretion, or DNA integrity of the superparamagnetic iron oxide‐labeled adipose‐derived stem cells (SPIOASCs). Implantation of adipose‐derived stem cells promotes myocardial neovascularization and inhibits ischemic cardiomyocyte apoptosis through endothelial differentiation, incorporation into vessels, and paracrine factor secretion. An externally applied static magnetic field enhanced myocardial retention of intramyocardially injected "magnetic" SPIOASCs and promoted cardiac function recovery after myocardial infarction. With further preclinical optimization, this approach may improve the outcome of current stem cell therapy for ischemic myocardial infarction.
Although adipose‐derived stem cells (ASCs) hold the promise of effective therapy for myocardial infarction (MI), low cardiac retention of implanted ASCs has hindered their therapeutic efficiency. We investigated whether an externally applied static magnetic field (SMF) enhanced cardiac localization of “magnetic” ASCs preloaded with superparamagnetic iron oxide (SPIO) nanoparticles and further improved heart function recovery. In conclusion, the SMF increased the cardiac retention of implanted “magnetic” SPIO‐labeled ASCs and enhanced heart function recovery after MI.</description><subject>Adipose Tissue - cytology</subject><subject>Adipose-derived stem cells</subject><subject>Administrative support</subject><subject>Angiogenesis</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Cardiac function</subject><subject>Cardiomyocytes</subject><subject>Cell culture</subject><subject>Cell Differentiation</subject><subject>Cell Movement</subject><subject>Cell retention</subject><subject>Cytokines</subject><subject>Data analysis</subject><subject>Deoxyribonucleic acid</subject><subject>Disease Models, Animal</subject><subject>DNA</subject><subject>Drug dosages</subject><subject>Endothelial cells</subject><subject>FDA approval</subject><subject>Female</subject><subject>Ferric Compounds - pharmacology</subject><subject>Flow Cytometry</subject><subject>Heart</subject><subject>Iron oxides</subject><subject>Ischemia</subject><subject>Localization</subject><subject>Lungs</subject><subject>Magnetic Fields</subject><subject>Male</subject><subject>Metal Nanoparticles</subject><subject>Muscle contraction</subject><subject>Myocardial Infarction</subject><subject>Nanoparticles</subject><subject>Paracrine signalling</subject><subject>Pilot Projects</subject><subject>Rats</subject><subject>Rats, Transgenic</subject><subject>Retention</subject><subject>Science</subject><subject>Static magnetic field</subject><subject>Stem Cell Transplantation - methods</subject><subject>Stem Cells</subject><subject>Studies</subject><subject>Superparamagnetic iron oxide</subject><subject>Tissue Engineering and Regenerative Medicine</subject><subject>Vascularization</subject><issn>2157-6564</issn><issn>2157-6580</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFUsGO0zAQjRCIXS175YgsceHSMnYS2xESUiktW6kIiV3OluNMdr1ynGKnC73xCXwHn8WX4LRLBVzwxTPj5zfzNC_LnlKYlhXnL6MZuikDWk6AMXiQnTJaigkvJTw8xrw4yc5jvIV0eMUrBo-zEyYKAFGJ0-zH4uuAwWvndmS22TiLDbkc9GANea-vPY7B0qJryMLfaG8wkrkOjdWGfMQB_WB7T7RvyHLrzZhoR96gx9YOpG-PHHv-Vej9z2_f17pGl9rMGrvpI6bKWwz2bt8YOzJH5yKxnqx8q4MZUv0CdRjik-xRq13E8_v7LPu0XFzNLybrD-9W89l6YgoBMJFS8rqpWgmMAqtEIQoOZZXnVSWxTqoBGigEZ0m_pLmRIApGm7LWyLEQZX6WvT7wbrZ1h41JIoN2ahNsp8NO9dqqv1-8vVHX_Z0qIad0T_DiniD0n7cYB9XZaJIs7bHfRkUl44IKyWWCPv8Hettvx3VExVgFNC9KxhJqekCZ0McYsD0OQ0GNTlCjE9ToBDU6IX149qeEI_z33hPg1QHwxTrc_YdOXc6v8pTxkqaJJOS_ANURw2Q</recordid><startdate>201610</startdate><enddate>201610</enddate><creator>Wang, Jian</creator><creator>Xiang, Bo</creator><creator>Deng, Jixian</creator><creator>Lin, Hung-Yu</creator><creator>Zheng, Dayang</creator><creator>Freed, Darren H.</creator><creator>Arora, Rakesh C.</creator><creator>Tian, Ganghong</creator><general>AlphaMed Press</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201610</creationdate><title>Externally Applied Static Magnetic Field Enhances Cardiac Retention and Functional Benefit of Magnetically Iron‐Labeled Adipose‐Derived Stem Cells in Infarcted Hearts</title><author>Wang, Jian ; Xiang, Bo ; Deng, Jixian ; Lin, Hung-Yu ; Zheng, Dayang ; Freed, Darren H. ; Arora, Rakesh C. ; Tian, Ganghong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4700-8886bd9f80210297474605933998eb07900d04762797813c807421d5bae6e4753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adipose Tissue - cytology</topic><topic>Adipose-derived stem cells</topic><topic>Administrative support</topic><topic>Angiogenesis</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Cardiac function</topic><topic>Cardiomyocytes</topic><topic>Cell culture</topic><topic>Cell Differentiation</topic><topic>Cell Movement</topic><topic>Cell retention</topic><topic>Cytokines</topic><topic>Data analysis</topic><topic>Deoxyribonucleic acid</topic><topic>Disease Models, Animal</topic><topic>DNA</topic><topic>Drug dosages</topic><topic>Endothelial cells</topic><topic>FDA approval</topic><topic>Female</topic><topic>Ferric Compounds - pharmacology</topic><topic>Flow Cytometry</topic><topic>Heart</topic><topic>Iron oxides</topic><topic>Ischemia</topic><topic>Localization</topic><topic>Lungs</topic><topic>Magnetic Fields</topic><topic>Male</topic><topic>Metal Nanoparticles</topic><topic>Muscle contraction</topic><topic>Myocardial Infarction</topic><topic>Nanoparticles</topic><topic>Paracrine signalling</topic><topic>Pilot Projects</topic><topic>Rats</topic><topic>Rats, Transgenic</topic><topic>Retention</topic><topic>Science</topic><topic>Static magnetic field</topic><topic>Stem Cell Transplantation - methods</topic><topic>Stem Cells</topic><topic>Studies</topic><topic>Superparamagnetic iron oxide</topic><topic>Tissue Engineering and Regenerative Medicine</topic><topic>Vascularization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Jian</creatorcontrib><creatorcontrib>Xiang, Bo</creatorcontrib><creatorcontrib>Deng, Jixian</creatorcontrib><creatorcontrib>Lin, Hung-Yu</creatorcontrib><creatorcontrib>Zheng, Dayang</creatorcontrib><creatorcontrib>Freed, Darren H.</creatorcontrib><creatorcontrib>Arora, Rakesh C.</creatorcontrib><creatorcontrib>Tian, Ganghong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Stem cells translational medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Wang, Jian</au><au>Xiang, Bo</au><au>Deng, Jixian</au><au>Lin, Hung-Yu</au><au>Zheng, Dayang</au><au>Freed, Darren H.</au><au>Arora, Rakesh C.</au><au>Tian, Ganghong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Externally Applied Static Magnetic Field Enhances Cardiac Retention and Functional Benefit of Magnetically Iron‐Labeled Adipose‐Derived Stem Cells in Infarcted Hearts</atitle><jtitle>Stem cells translational medicine</jtitle><addtitle>Stem Cells Transl Med</addtitle><date>2016-10</date><risdate>2016</risdate><volume>5</volume><issue>10</issue><spage>1380</spage><epage>1393</epage><pages>1380-1393</pages><issn>2157-6564</issn><eissn>2157-6580</eissn><abstract>Although adipose‐derived stem cells (ASCs) hold the promise of effective therapy for myocardial infarction, low cardiac retention of implanted ASCs has hindered their therapeutic efficiency. We investigated whether an externally applied static magnetic field (SMF) enhances cardiac localization of "magnetic" cells and promotes heart function recovery when ASCs are preloaded with superparamagnetic iron oxide (SPIO) nanoparticles. The influence of SMF (0.1 Tesla) on the biological activities of SPIO‐labeled ASCs (SPIOASCs) was investigated first. Fifty‐six female rats with myocardial infarction underwent intramyocardial injection of cell culture medium (CCM) or male SPIOASCs with or without the subcutaneous implantable magnet (CCM‐magnet or SPIOASC‐magnet). Four weeks later, endothelial differentiation, angiogenic cytokine secretion, angiogenesis, cardiomyocyte apoptosis, cell retention, and cardiac performance were examined. The 0.1‐Tsela SMF did not adversely affect the viability, proliferation, angiogenic cytokine secretion, and DNA integrity of SPIOASCs. The implanted SPIOASCs could differentiate into endothelial cell, incorporate into newly formed vessels, and secrete multiple angiogenic cytokines. Four weeks after cell transplantation, the number of cardiac SPIOASCs was significantly increased, vascular density was markedly enlarged, fewer apoptotic cardiomyocytes were present, and heart contractile function was substantially improved in the SPIOASC‐magnet treated rats in comparison with the SPIOASC‐treated rats. The SPIOASCs could differentiate into endothelial cells, incorporate into vessels, promote angiogenesis, and inhibit ischemic cardiomyocyte apoptosis. An externally applied SMF offered a secure environment for biological properties of SPIOASCs, increased the cardiac retention of implanted magnetic SPIOASCs, and further enhanced heart function recovery after myocardial infarction.
Significance
This pilot proof‐of‐concept study suggests that a 0.1‐Tesla static magnetic field does not adversely affect the viability, proliferation, angiogenic cytokine secretion, or DNA integrity of the superparamagnetic iron oxide‐labeled adipose‐derived stem cells (SPIOASCs). Implantation of adipose‐derived stem cells promotes myocardial neovascularization and inhibits ischemic cardiomyocyte apoptosis through endothelial differentiation, incorporation into vessels, and paracrine factor secretion. An externally applied static magnetic field enhanced myocardial retention of intramyocardially injected "magnetic" SPIOASCs and promoted cardiac function recovery after myocardial infarction. With further preclinical optimization, this approach may improve the outcome of current stem cell therapy for ischemic myocardial infarction.
Although adipose‐derived stem cells (ASCs) hold the promise of effective therapy for myocardial infarction (MI), low cardiac retention of implanted ASCs has hindered their therapeutic efficiency. We investigated whether an externally applied static magnetic field (SMF) enhanced cardiac localization of “magnetic” ASCs preloaded with superparamagnetic iron oxide (SPIO) nanoparticles and further improved heart function recovery. In conclusion, the SMF increased the cardiac retention of implanted “magnetic” SPIO‐labeled ASCs and enhanced heart function recovery after MI.</abstract><cop>Durham, NC, USA</cop><pub>AlphaMed Press</pub><pmid>27400797</pmid><doi>10.5966/sctm.2015-0220</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Stem cells translational medicine, 2016-10, Vol.5 (10), p.1380-1393 |
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| subjects | Adipose Tissue - cytology Adipose-derived stem cells Administrative support Angiogenesis Animals Apoptosis Cardiac function Cardiomyocytes Cell culture Cell Differentiation Cell Movement Cell retention Cytokines Data analysis Deoxyribonucleic acid Disease Models, Animal DNA Drug dosages Endothelial cells FDA approval Female Ferric Compounds - pharmacology Flow Cytometry Heart Iron oxides Ischemia Localization Lungs Magnetic Fields Male Metal Nanoparticles Muscle contraction Myocardial Infarction Nanoparticles Paracrine signalling Pilot Projects Rats Rats, Transgenic Retention Science Static magnetic field Stem Cell Transplantation - methods Stem Cells Studies Superparamagnetic iron oxide Tissue Engineering and Regenerative Medicine Vascularization |
| title | Externally Applied Static Magnetic Field Enhances Cardiac Retention and Functional Benefit of Magnetically Iron‐Labeled Adipose‐Derived Stem Cells in Infarcted Hearts |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T21%3A58%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_24P&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Externally%20Applied%20Static%20Magnetic%20Field%20Enhances%20Cardiac%20Retention%20and%20Functional%20Benefit%20of%20Magnetically%20Iron%E2%80%90Labeled%20Adipose%E2%80%90Derived%20Stem%20Cells%20in%20Infarcted%20Hearts&rft.jtitle=Stem%20cells%20translational%20medicine&rft.au=Wang,%20Jian&rft.date=2016-10&rft.volume=5&rft.issue=10&rft.spage=1380&rft.epage=1393&rft.pages=1380-1393&rft.issn=2157-6564&rft.eissn=2157-6580&rft_id=info:doi/10.5966/sctm.2015-0220&rft_dat=%3Cproquest_24P%3E1826717868%3C/proquest_24P%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2290134522&rft_id=info:pmid/27400797&rfr_iscdi=true |