A rapid polymerase chain reaction technique for detecting M tuberculosis in a variety of clinical specimens
A rapid in-house polymerase chain reaction (PCR) assay is described for the direct detection of Mycobacterium tuberculosis complex in clinical material. Its performance is compared with two kit based systems. The results of the in-house assay were comparable with the commercial assays, detecting M t...
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| Veröffentlicht in: | Journal of clinical pathology 1998-12, Vol.51 (12), p.922-924 |
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| container_end_page | 924 |
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| container_issue | 12 |
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| container_title | Journal of clinical pathology |
| container_volume | 51 |
| creator | Kearns, A M Freeman, R Steward, M Magee, J G |
| description | A rapid in-house polymerase chain reaction (PCR) assay is described for the direct detection of Mycobacterium tuberculosis complex in clinical material. Its performance is compared with two kit based systems. The results of the in-house assay were comparable with the commercial assays, detecting M tuberculosis in 100% of smear positive, culture positive samples. The in-house assay proved to be rapid, easy, and inexpensive to perform, and the inclusion of an internal inhibitor control permitted validation of the PCR results. |
| doi_str_mv | 10.1136/jcp.51.12.922 |
| format | Article |
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Its performance is compared with two kit based systems. The results of the in-house assay were comparable with the commercial assays, detecting M tuberculosis in 100% of smear positive, culture positive samples. 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Its performance is compared with two kit based systems. The results of the in-house assay were comparable with the commercial assays, detecting M tuberculosis in 100% of smear positive, culture positive samples. The in-house assay proved to be rapid, easy, and inexpensive to perform, and the inclusion of an internal inhibitor control permitted validation of the PCR results.</description><subject>Biological and medical sciences</subject><subject>Bronchoalveolar Lavage Fluid - microbiology</subject><subject>DNA, Bacterial - cerebrospinal fluid</subject><subject>DNA, Bacterial - isolation & purification</subject><subject>DNA, Bacterial - urine</subject><subject>Humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Lymph Nodes - microbiology</subject><subject>Medical sciences</subject><subject>Miscellaneous. Technology</subject><subject>Mycobacterium tuberculosis - genetics</subject><subject>Mycobacterium tuberculosis - isolation & purification</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Reagent Kits, Diagnostic - standards</subject><subject>Sensitivity and Specificity</subject><subject>Sputum - microbiology</subject><issn>0021-9746</issn><issn>1472-4146</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkbtvFDEQxi0EIpdASYtcIESzh1_7KiiSEwRQACGFtNasd5zzZdde7L0o999jdKckVFSj0fy-eX2EvOJsybms3m_MtCz5kotlK8QTsuCqFoXiqnpKFowJXrS1qo7IcUobxrisuXxOjjhjNZNSLcjNKY0wuZ5OYdiNGCEhNWtwnkYEM7vg6Yxm7d3vLVIbIu0x57Pz1_QbnbcdRrMdQnKJZgnQW4gO5x0NlprBeWdgoGlC40b06QV5ZmFI-PIQT8ivTx8vV5-Lix_nX1anF0WnGjEXDXSdkV0nWtmA7a00tmxaUykLHa-ZqrDswZbIWuilsAYrUaKSNTM99qwp5Qn5sO87bbsRe4N-jjDoKboR4k4HcPrfindrfR1udck4E03Wvz3oY8hnp1mPLhkcBvAYtklXLW-4UCyDxR40MaQU0d7P4Ez_dUdnd3TJNRc6u5P5148Xe0Tv7cjAmwMAKb_ORvDGpQeukoo19cNcl2a8uy9DvNFVLetSf79a6bPzy68_z5orzTP_bs934-Y_K_4BzuC3mA</recordid><startdate>19981201</startdate><enddate>19981201</enddate><creator>Kearns, A M</creator><creator>Freeman, R</creator><creator>Steward, M</creator><creator>Magee, J G</creator><general>BMJ Publishing Group Ltd and Association of Clinical Pathologists</general><general>BMJ</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19981201</creationdate><title>A rapid polymerase chain reaction technique for detecting M tuberculosis in a variety of clinical specimens</title><author>Kearns, A M ; Freeman, R ; Steward, M ; Magee, J G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b482t-8abbc3bb2938afdf3cf589c64fab17046e5daf5e09ad32fce625e4370cded0853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Biological and medical sciences</topic><topic>Bronchoalveolar Lavage Fluid - microbiology</topic><topic>DNA, Bacterial - cerebrospinal fluid</topic><topic>DNA, Bacterial - isolation & purification</topic><topic>DNA, Bacterial - urine</topic><topic>Humans</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Lymph Nodes - microbiology</topic><topic>Medical sciences</topic><topic>Miscellaneous. Technology</topic><topic>Mycobacterium tuberculosis - genetics</topic><topic>Mycobacterium tuberculosis - isolation & purification</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Reagent Kits, Diagnostic - standards</topic><topic>Sensitivity and Specificity</topic><topic>Sputum - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kearns, A M</creatorcontrib><creatorcontrib>Freeman, R</creatorcontrib><creatorcontrib>Steward, M</creatorcontrib><creatorcontrib>Magee, J G</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of clinical pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kearns, A M</au><au>Freeman, R</au><au>Steward, M</au><au>Magee, J G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A rapid polymerase chain reaction technique for detecting M tuberculosis in a variety of clinical specimens</atitle><jtitle>Journal of clinical pathology</jtitle><addtitle>J Clin Pathol</addtitle><date>1998-12-01</date><risdate>1998</risdate><volume>51</volume><issue>12</issue><spage>922</spage><epage>924</epage><pages>922-924</pages><issn>0021-9746</issn><eissn>1472-4146</eissn><coden>JCPAAK</coden><abstract>A rapid in-house polymerase chain reaction (PCR) assay is described for the direct detection of Mycobacterium tuberculosis complex in clinical material. Its performance is compared with two kit based systems. The results of the in-house assay were comparable with the commercial assays, detecting M tuberculosis in 100% of smear positive, culture positive samples. The in-house assay proved to be rapid, easy, and inexpensive to perform, and the inclusion of an internal inhibitor control permitted validation of the PCR results.</abstract><cop>London</cop><pub>BMJ Publishing Group Ltd and Association of Clinical Pathologists</pub><pmid>10070334</pmid><doi>10.1136/jcp.51.12.922</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of clinical pathology, 1998-12, Vol.51 (12), p.922-924 |
| issn | 0021-9746 1472-4146 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Biological and medical sciences Bronchoalveolar Lavage Fluid - microbiology DNA, Bacterial - cerebrospinal fluid DNA, Bacterial - isolation & purification DNA, Bacterial - urine Humans Investigative techniques, diagnostic techniques (general aspects) Lymph Nodes - microbiology Medical sciences Miscellaneous. Technology Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - isolation & purification Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Polymerase Chain Reaction - methods Reagent Kits, Diagnostic - standards Sensitivity and Specificity Sputum - microbiology |
| title | A rapid polymerase chain reaction technique for detecting M tuberculosis in a variety of clinical specimens |
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