A rapid polymerase chain reaction technique for detecting M tuberculosis in a variety of clinical specimens

A rapid in-house polymerase chain reaction (PCR) assay is described for the direct detection of Mycobacterium tuberculosis complex in clinical material. Its performance is compared with two kit based systems. The results of the in-house assay were comparable with the commercial assays, detecting M t...

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Veröffentlicht in:	Journal of clinical pathology 1998-12, Vol.51 (12), p.922-924
Hauptverfasser:	Kearns, A M, Freeman, R, Steward, M, Magee, J G
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Bronchoalveolar Lavage Fluid - microbiology DNA, Bacterial - cerebrospinal fluid DNA, Bacterial - isolation & purification DNA, Bacterial - urine Humans Investigative techniques, diagnostic techniques (general aspects) Lymph Nodes - microbiology Medical sciences Miscellaneous. Technology Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - isolation & purification Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Polymerase Chain Reaction - methods Reagent Kits, Diagnostic - standards Sensitivity and Specificity Sputum - microbiology
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Beschreibung
Zusammenfassung:	A rapid in-house polymerase chain reaction (PCR) assay is described for the direct detection of Mycobacterium tuberculosis complex in clinical material. Its performance is compared with two kit based systems. The results of the in-house assay were comparable with the commercial assays, detecting M tuberculosis in 100% of smear positive, culture positive samples. The in-house assay proved to be rapid, easy, and inexpensive to perform, and the inclusion of an internal inhibitor control permitted validation of the PCR results.
ISSN:	0021-9746 1472-4146
DOI:	10.1136/jcp.51.12.922