Direct high‐throughput amplification and sequencing of immunoglobulin genes from single human B cells

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Bibliographische Detailangaben
Veröffentlicht in:	European journal of immunology 2015-09, Vol.45 (9), p.2698-2700
Hauptverfasser:	Murugan, Rajagopal, Imkeller, Katharina, Busse, Christian E., Wardemann, Hedda
Format:	Artikel
Sprache:	eng
Schlagworte:	B cell B-Lymphocytes - cytology B-Lymphocytes - immunology B-Lymphocytes - metabolism DNA Primers - chemical synthesis DNA Primers - metabolism Genes, Immunoglobulin High-Throughput Nucleotide Sequencing Human antibody repertoire Humans Ig heavy chain Ig light chain Immunoglobulin Heavy Chains - genetics Immunoglobulin Heavy Chains - immunology Immunoglobulin Light Chains - genetics Immunoglobulin Light Chains - immunology Multiplex Polymerase Chain Reaction - methods Single cell PCR Single-Cell Analysis Technical Comment
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