Sensitive and universal method for microbial DNA extraction from blood products

A new method of extracting bacterial and yeast DNA from blood products dependent on guanidinium thiocyanate acid extraction and proteinase K treatment is described. In spiked samples the sensitivity per 0.1 ml of serum and blood, respectively, was 26 and 150 colony forming units (cfu) for Escherichi...

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Veröffentlicht in:	Journal of clinical pathology 1996-10, Vol.49 (10), p.861-863
Hauptverfasser:	Golbang, N, Burnie, J P, Klapper, P E, Bostock, A, Williamson, P
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Blood - microbiology Candida albicans - isolation & purification DNA, Bacterial - isolation & purification Escherichia coli - isolation & purification Humans Investigative techniques, diagnostic techniques (general aspects) Medical sciences Microbiological Techniques Miscellaneous. Technology Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Polymerase Chain Reaction Sensitivity and Specificity Staphylococcus aureus - isolation & purification
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Beschreibung
Zusammenfassung:	A new method of extracting bacterial and yeast DNA from blood products dependent on guanidinium thiocyanate acid extraction and proteinase K treatment is described. In spiked samples the sensitivity per 0.1 ml of serum and blood, respectively, was 26 and 150 colony forming units (cfu) for Escherichia coli, 80 and 120 cfu for Staphylococcus aureus and 20 and 26 cfu for Candida albicans. This compared well with existing methodologies, worked on limited clinical samples and was not pathogen specific.
ISSN:	0021-9746 1472-4146
DOI:	10.1136/jcp.49.10.861