In vitro selection and amplification protocols for isolation of aptameric sensors for small molecules
•Highly useful method for the selection of aptamers for small-molecule targets.•Provides a very detail step-wise guide to a selection protocol.•Simple enough to be readily implemented in a non-specialist laboratory. We recently optimized a procedure that directly yields aptameric sensors for small m...
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| Veröffentlicht in: | Methods (San Diego, Calif.) Calif.), 2016-08, Vol.106, p.58-65 |
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| container_title | Methods (San Diego, Calif.) |
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| creator | Yang, Kyung-Ae Pei, Renjun Stojanovic, Milan N. |
| description | •Highly useful method for the selection of aptamers for small-molecule targets.•Provides a very detail step-wise guide to a selection protocol.•Simple enough to be readily implemented in a non-specialist laboratory.
We recently optimized a procedure that directly yields aptameric sensors for small molecules in so-called structure-switching format. The protocol has a high success rate, short time, and is sufficiently simple to be readily implemented in a non-specialist laboratory. We provide a stepwise guide to this selection protocol. |
| doi_str_mv | 10.1016/j.ymeth.2016.04.032 |
| format | Article |
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| identifier | ISSN: 1046-2023 |
| ispartof | Methods (San Diego, Calif.), 2016-08, Vol.106, p.58-65 |
| issn | 1046-2023 1095-9130 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4981533 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Aptamer Aptamers, Nucleotide - chemistry Aptamers, Nucleotide - genetics Biosensing Techniques - methods Biosensor Nucleic Acid Conformation SELEX SELEX Aptamer Technique - methods Small Molecule Libraries - chemistry Small molecules Structure-switching |
| title | In vitro selection and amplification protocols for isolation of aptameric sensors for small molecules |
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