In vitro selection and amplification protocols for isolation of aptameric sensors for small molecules

•Highly useful method for the selection of aptamers for small-molecule targets.•Provides a very detail step-wise guide to a selection protocol.•Simple enough to be readily implemented in a non-specialist laboratory. We recently optimized a procedure that directly yields aptameric sensors for small m...

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Veröffentlicht in:Methods (San Diego, Calif.) Calif.), 2016-08, Vol.106, p.58-65
Hauptverfasser: Yang, Kyung-Ae, Pei, Renjun, Stojanovic, Milan N.
Format: Artikel
Sprache:eng
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Zusammenfassung:•Highly useful method for the selection of aptamers for small-molecule targets.•Provides a very detail step-wise guide to a selection protocol.•Simple enough to be readily implemented in a non-specialist laboratory. We recently optimized a procedure that directly yields aptameric sensors for small molecules in so-called structure-switching format. The protocol has a high success rate, short time, and is sufficiently simple to be readily implemented in a non-specialist laboratory. We provide a stepwise guide to this selection protocol.
ISSN:1046-2023
1095-9130
DOI:10.1016/j.ymeth.2016.04.032