In vitro selection and amplification protocols for isolation of aptameric sensors for small molecules
•Highly useful method for the selection of aptamers for small-molecule targets.•Provides a very detail step-wise guide to a selection protocol.•Simple enough to be readily implemented in a non-specialist laboratory. We recently optimized a procedure that directly yields aptameric sensors for small m...
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Veröffentlicht in: | Methods (San Diego, Calif.) Calif.), 2016-08, Vol.106, p.58-65 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | •Highly useful method for the selection of aptamers for small-molecule targets.•Provides a very detail step-wise guide to a selection protocol.•Simple enough to be readily implemented in a non-specialist laboratory.
We recently optimized a procedure that directly yields aptameric sensors for small molecules in so-called structure-switching format. The protocol has a high success rate, short time, and is sufficiently simple to be readily implemented in a non-specialist laboratory. We provide a stepwise guide to this selection protocol. |
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ISSN: | 1046-2023 1095-9130 |
DOI: | 10.1016/j.ymeth.2016.04.032 |