Tunable, biodegradable gold nanoparticles as contrast agents for computed tomography and photoacoustic imaging
Abstract Gold nanoparticles (AuNP) have been proposed for many applications in medicine. Although large AuNP (>5.5 nm) are desirable for their longer blood circulation and accumulation in diseased tissues, small AuNP (
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| Veröffentlicht in: | Biomaterials 2016-09, Vol.102, p.87-97 |
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| creator | Cheheltani, Rabee Ezzibdeh, Rami M Chhour, Peter Pulaparthi, Kumidini Kim, Johoon Jurcova, Martina Hsu, Jessica C Blundell, Cassidy Litt, Harold I Ferrari, Victor A Allcock, Harry R Sehgal, Chandra M Cormode, David P |
| description | Abstract Gold nanoparticles (AuNP) have been proposed for many applications in medicine. Although large AuNP (>5.5 nm) are desirable for their longer blood circulation and accumulation in diseased tissues, small AuNP ( |
| doi_str_mv | 10.1016/j.biomaterials.2016.06.015 |
| format | Article |
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Although large AuNP (>5.5 nm) are desirable for their longer blood circulation and accumulation in diseased tissues, small AuNP (<5.5 nm) are required for excretion via the kidneys. We present a novel platform where small, excretable AuNP are encapsulated into biodegradable poly di(carboxylatophenoxy)phosphazene (PCPP) nanospheres. These larger nanoparticles (Au-PCPP) can perform their function as contrast agents, then subsequently break down into harmless byproducts and release the AuNP for swift excretion. Homogeneous Au-PCPP were synthesized using a microfluidic device. The size of the Au-PCPP can be controlled by the amount of polyethylene glycol-polylysine (PEG-PLL) block co-polymer in the formulation. Synthesis of Au-PCPP nanoparticles and encapsulation of AuNP in PCPP were evaluated using transmission electron microscopy and their biocompatibility and biodegradability confirmed in vitro . The Au-PCPP nanoparticles were found to produce strong computed tomography contrast. The UV–Vis absorption peak of Au-PCPP can be tuned into the near infrared region via inclusion of varying amounts of AuNP and controlling the nanoparticle size. In vitro and in vivo experiments demonstrated the potential of Au-PCPP as contrast agents for photoacoustic imaging. Therefore, Au-PCPP nanoparticles have high potency as contrast agents for two imaging modalities, as well as being biocompatible and biodegradable, and thus represent a platform with potential for translation into the clinic.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2016.06.015</identifier><identifier>PMID: 27322961</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Advanced Basic Science ; Animals ; Biocompatibility ; Biodegradability ; blood circulation ; byproducts ; Cell Line ; composite polymers ; Computed tomography ; Contrast agent ; Contrast agents ; Contrast Media - chemistry ; Dentistry ; encapsulation ; Excretion ; Gold ; Gold - chemistry ; Gold nanoparticle ; Humans ; image analysis ; Imaging ; In vitro testing ; in vivo studies ; kidneys ; medicine ; Metal Nanoparticles - chemistry ; Metal Nanoparticles - ultrastructure ; Metal-polymer nanoparticle ; Mice, Inbred C57BL ; nanogold ; Nanoparticles ; nanospheres ; Organophosphorus Compounds - chemistry ; Photoacoustic imaging ; Photoacoustic Techniques - methods ; polyethylene ; Polymers - chemistry ; tissues ; Tomography, X-Ray Computed - methods ; transmission electron microscopy ; ultraviolet-visible spectroscopy</subject><ispartof>Biomaterials, 2016-09, Vol.102, p.87-97</ispartof><rights>Elsevier Ltd</rights><rights>2016 Elsevier Ltd</rights><rights>Copyright © 2016 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c641t-4a8856980f2f6731441ebaa00a315ecc073208508dd27bb839b0ec922a0b50413</citedby><cites>FETCH-LOGICAL-c641t-4a8856980f2f6731441ebaa00a315ecc073208508dd27bb839b0ec922a0b50413</cites><orcidid>0000-0002-2633-4442</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0142961216302691$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27322961$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cheheltani, Rabee</creatorcontrib><creatorcontrib>Ezzibdeh, Rami M</creatorcontrib><creatorcontrib>Chhour, Peter</creatorcontrib><creatorcontrib>Pulaparthi, Kumidini</creatorcontrib><creatorcontrib>Kim, Johoon</creatorcontrib><creatorcontrib>Jurcova, Martina</creatorcontrib><creatorcontrib>Hsu, Jessica C</creatorcontrib><creatorcontrib>Blundell, Cassidy</creatorcontrib><creatorcontrib>Litt, Harold I</creatorcontrib><creatorcontrib>Ferrari, Victor A</creatorcontrib><creatorcontrib>Allcock, Harry R</creatorcontrib><creatorcontrib>Sehgal, Chandra M</creatorcontrib><creatorcontrib>Cormode, David P</creatorcontrib><title>Tunable, biodegradable gold nanoparticles as contrast agents for computed tomography and photoacoustic imaging</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Abstract Gold nanoparticles (AuNP) have been proposed for many applications in medicine. Although large AuNP (>5.5 nm) are desirable for their longer blood circulation and accumulation in diseased tissues, small AuNP (<5.5 nm) are required for excretion via the kidneys. We present a novel platform where small, excretable AuNP are encapsulated into biodegradable poly di(carboxylatophenoxy)phosphazene (PCPP) nanospheres. These larger nanoparticles (Au-PCPP) can perform their function as contrast agents, then subsequently break down into harmless byproducts and release the AuNP for swift excretion. Homogeneous Au-PCPP were synthesized using a microfluidic device. The size of the Au-PCPP can be controlled by the amount of polyethylene glycol-polylysine (PEG-PLL) block co-polymer in the formulation. Synthesis of Au-PCPP nanoparticles and encapsulation of AuNP in PCPP were evaluated using transmission electron microscopy and their biocompatibility and biodegradability confirmed in vitro . The Au-PCPP nanoparticles were found to produce strong computed tomography contrast. The UV–Vis absorption peak of Au-PCPP can be tuned into the near infrared region via inclusion of varying amounts of AuNP and controlling the nanoparticle size. In vitro and in vivo experiments demonstrated the potential of Au-PCPP as contrast agents for photoacoustic imaging. Therefore, Au-PCPP nanoparticles have high potency as contrast agents for two imaging modalities, as well as being biocompatible and biodegradable, and thus represent a platform with potential for translation into the clinic.</description><subject>Advanced Basic Science</subject><subject>Animals</subject><subject>Biocompatibility</subject><subject>Biodegradability</subject><subject>blood circulation</subject><subject>byproducts</subject><subject>Cell Line</subject><subject>composite polymers</subject><subject>Computed tomography</subject><subject>Contrast agent</subject><subject>Contrast agents</subject><subject>Contrast Media - chemistry</subject><subject>Dentistry</subject><subject>encapsulation</subject><subject>Excretion</subject><subject>Gold</subject><subject>Gold - chemistry</subject><subject>Gold nanoparticle</subject><subject>Humans</subject><subject>image analysis</subject><subject>Imaging</subject><subject>In vitro testing</subject><subject>in vivo studies</subject><subject>kidneys</subject><subject>medicine</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Metal Nanoparticles - ultrastructure</subject><subject>Metal-polymer nanoparticle</subject><subject>Mice, Inbred C57BL</subject><subject>nanogold</subject><subject>Nanoparticles</subject><subject>nanospheres</subject><subject>Organophosphorus Compounds - chemistry</subject><subject>Photoacoustic imaging</subject><subject>Photoacoustic Techniques - methods</subject><subject>polyethylene</subject><subject>Polymers - chemistry</subject><subject>tissues</subject><subject>Tomography, X-Ray Computed - methods</subject><subject>transmission electron microscopy</subject><subject>ultraviolet-visible spectroscopy</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUk1v1DAQtRCIloW_gCxOHNhl7NhZh0MlVD6lShwoZ8txZrNeEjvYTqX99zjaUhUuVBrJmpn3xm_sR8grBhsGrH572LQujCZjdGZIG15qGyjB5CNyztRWrWUD8jE5Byb4uqkZPyPPUjpAyUHwp-SMbyvOS-Oc-OvZm3bAN7TM7LCPpltS2oeho974MJmYnR0wUZOoDT5HkzI1Pfqc6C7EUhunOWNHcxhD4U_7IzW-o9M-5GBsmFPhUzea3vn-OXmyK5rxxe25Ij8-fby-_LK--vb56-X7q7WtBctrYZSSdaNgx3f1tmJCMGyNATAVk2gtFP2gJKiu49u2VVXTAtqGcwOtBMGqFbk4zZ3mdsTO4qJ70FMsOuJRB-P03x3v9roPN1o0gtXleVbk9e2AGH7NmLIeXbI4DMZjWUlzAOBVxVT1XyhTXEq55eIhUFC1kGWxAn13gtoYUoq4uxPPQC820Ad93wZ6sYGGEkwW8sv7699R__x7AXw4AbB8wo3DqJN16C12LqLNugvuYfdc_DPGDs47a4afeMR0CHP0C4fpxDXo74shFz-yugJeN6z6DQM94W0</recordid><startdate>20160901</startdate><enddate>20160901</enddate><creator>Cheheltani, Rabee</creator><creator>Ezzibdeh, Rami M</creator><creator>Chhour, Peter</creator><creator>Pulaparthi, Kumidini</creator><creator>Kim, Johoon</creator><creator>Jurcova, Martina</creator><creator>Hsu, Jessica C</creator><creator>Blundell, Cassidy</creator><creator>Litt, Harold I</creator><creator>Ferrari, Victor A</creator><creator>Allcock, Harry R</creator><creator>Sehgal, Chandra M</creator><creator>Cormode, David P</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>F28</scope><scope>JG9</scope><scope>L7M</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-2633-4442</orcidid></search><sort><creationdate>20160901</creationdate><title>Tunable, biodegradable gold nanoparticles as contrast agents for computed tomography and photoacoustic imaging</title><author>Cheheltani, Rabee ; Ezzibdeh, Rami M ; Chhour, Peter ; Pulaparthi, Kumidini ; Kim, Johoon ; Jurcova, Martina ; Hsu, Jessica C ; Blundell, Cassidy ; Litt, Harold I ; Ferrari, Victor A ; Allcock, Harry R ; Sehgal, Chandra M ; Cormode, David P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c641t-4a8856980f2f6731441ebaa00a315ecc073208508dd27bb839b0ec922a0b50413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Advanced Basic Science</topic><topic>Animals</topic><topic>Biocompatibility</topic><topic>Biodegradability</topic><topic>blood circulation</topic><topic>byproducts</topic><topic>Cell Line</topic><topic>composite polymers</topic><topic>Computed tomography</topic><topic>Contrast agent</topic><topic>Contrast agents</topic><topic>Contrast Media - chemistry</topic><topic>Dentistry</topic><topic>encapsulation</topic><topic>Excretion</topic><topic>Gold</topic><topic>Gold - chemistry</topic><topic>Gold nanoparticle</topic><topic>Humans</topic><topic>image analysis</topic><topic>Imaging</topic><topic>In vitro testing</topic><topic>in vivo studies</topic><topic>kidneys</topic><topic>medicine</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Metal Nanoparticles - ultrastructure</topic><topic>Metal-polymer nanoparticle</topic><topic>Mice, Inbred C57BL</topic><topic>nanogold</topic><topic>Nanoparticles</topic><topic>nanospheres</topic><topic>Organophosphorus Compounds - chemistry</topic><topic>Photoacoustic imaging</topic><topic>Photoacoustic Techniques - methods</topic><topic>polyethylene</topic><topic>Polymers - chemistry</topic><topic>tissues</topic><topic>Tomography, X-Ray Computed - methods</topic><topic>transmission electron microscopy</topic><topic>ultraviolet-visible spectroscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cheheltani, Rabee</creatorcontrib><creatorcontrib>Ezzibdeh, Rami M</creatorcontrib><creatorcontrib>Chhour, Peter</creatorcontrib><creatorcontrib>Pulaparthi, Kumidini</creatorcontrib><creatorcontrib>Kim, Johoon</creatorcontrib><creatorcontrib>Jurcova, Martina</creatorcontrib><creatorcontrib>Hsu, Jessica C</creatorcontrib><creatorcontrib>Blundell, Cassidy</creatorcontrib><creatorcontrib>Litt, Harold I</creatorcontrib><creatorcontrib>Ferrari, Victor A</creatorcontrib><creatorcontrib>Allcock, Harry R</creatorcontrib><creatorcontrib>Sehgal, Chandra M</creatorcontrib><creatorcontrib>Cormode, David P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cheheltani, Rabee</au><au>Ezzibdeh, Rami M</au><au>Chhour, Peter</au><au>Pulaparthi, Kumidini</au><au>Kim, Johoon</au><au>Jurcova, Martina</au><au>Hsu, Jessica C</au><au>Blundell, Cassidy</au><au>Litt, Harold I</au><au>Ferrari, Victor A</au><au>Allcock, Harry R</au><au>Sehgal, Chandra M</au><au>Cormode, David P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tunable, biodegradable gold nanoparticles as contrast agents for computed tomography and photoacoustic imaging</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2016-09-01</date><risdate>2016</risdate><volume>102</volume><spage>87</spage><epage>97</epage><pages>87-97</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>Abstract Gold nanoparticles (AuNP) have been proposed for many applications in medicine. Although large AuNP (>5.5 nm) are desirable for their longer blood circulation and accumulation in diseased tissues, small AuNP (<5.5 nm) are required for excretion via the kidneys. We present a novel platform where small, excretable AuNP are encapsulated into biodegradable poly di(carboxylatophenoxy)phosphazene (PCPP) nanospheres. These larger nanoparticles (Au-PCPP) can perform their function as contrast agents, then subsequently break down into harmless byproducts and release the AuNP for swift excretion. Homogeneous Au-PCPP were synthesized using a microfluidic device. The size of the Au-PCPP can be controlled by the amount of polyethylene glycol-polylysine (PEG-PLL) block co-polymer in the formulation. Synthesis of Au-PCPP nanoparticles and encapsulation of AuNP in PCPP were evaluated using transmission electron microscopy and their biocompatibility and biodegradability confirmed in vitro . The Au-PCPP nanoparticles were found to produce strong computed tomography contrast. The UV–Vis absorption peak of Au-PCPP can be tuned into the near infrared region via inclusion of varying amounts of AuNP and controlling the nanoparticle size. In vitro and in vivo experiments demonstrated the potential of Au-PCPP as contrast agents for photoacoustic imaging. Therefore, Au-PCPP nanoparticles have high potency as contrast agents for two imaging modalities, as well as being biocompatible and biodegradable, and thus represent a platform with potential for translation into the clinic.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>27322961</pmid><doi>10.1016/j.biomaterials.2016.06.015</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-2633-4442</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Advanced Basic Science Animals Biocompatibility Biodegradability blood circulation byproducts Cell Line composite polymers Computed tomography Contrast agent Contrast agents Contrast Media - chemistry Dentistry encapsulation Excretion Gold Gold - chemistry Gold nanoparticle Humans image analysis Imaging In vitro testing in vivo studies kidneys medicine Metal Nanoparticles - chemistry Metal Nanoparticles - ultrastructure Metal-polymer nanoparticle Mice, Inbred C57BL nanogold Nanoparticles nanospheres Organophosphorus Compounds - chemistry Photoacoustic imaging Photoacoustic Techniques - methods polyethylene Polymers - chemistry tissues Tomography, X-Ray Computed - methods transmission electron microscopy ultraviolet-visible spectroscopy |
| title | Tunable, biodegradable gold nanoparticles as contrast agents for computed tomography and photoacoustic imaging |
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