Small Glutamine-Rich Tetratricopeptide Repeat-Containing Protein Alpha (SGTA) Ablation Limits Offspring Viability and Growth in Mice
Small glutamine-rich tetratricopeptide repeat-containing protein α (SGTA) has been implicated as a co-chaperone and regulator of androgen and growth hormone receptor (AR, GHR) signalling. We investigated the functional consequences of partial and full Sgta ablation in vivo using Cre-lox Sgta -null m...
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| Veröffentlicht in: | Scientific reports 2016-06, Vol.6 (1), p.28950-28950, Article 28950 |
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| creator | Philp, Lisa K. Day, Tanya K. Butler, Miriam S. Laven-Law, Geraldine Jindal, Shalini Hickey, Theresa E. Scher, Howard I. Butler, Lisa M. Tilley, Wayne D. |
| description | Small glutamine-rich tetratricopeptide repeat-containing protein α (SGTA) has been implicated as a co-chaperone and regulator of androgen and growth hormone receptor (AR, GHR) signalling. We investigated the functional consequences of partial and full
Sgta
ablation
in vivo
using Cre-lox
Sgta
-null mice.
Sgta
+/−
breeders generated viable
Sgta
−/−
offspring, but at less than Mendelian expectancy. S
gta
−/−
breeders were subfertile with small litters and higher neonatal death (
P
|
| doi_str_mv | 10.1038/srep28950 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4928056</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1801439094</sourcerecordid><originalsourceid>FETCH-LOGICAL-c504t-9b9588447073c050e7dc5a1efcb0a8613d9ec0ad5460dc8440ba1605b33db9433</originalsourceid><addsrcrecordid>eNplkUFrGzEQhUVpaYKTQ_9AEfSSFDaRVit7dSkY0zoFl5TE7VVotbOxglbaSNqW3PvDI-PUuI0uI9A3b97oIfSOkgtKWH0ZAwxlLTh5hY5LUvGiZGX5-uB-hE5jvCf58FJUVLxFR-WM8ZoKeoz-3PbKWry0Y1K9cVDcGL3Ba0hBpWC0H2BIpgV8AwOoVCy8S8o44-7w9-ATGIfndtgofHa7XM_P8byxKhnv8Mr0JkV83XVxCFv8p1GNsSY9YuVavAz-d9rg3P7NaDhBbzplI5w-1wn68eXzenFVrK6XXxfzVaE5qVIhGsHruqpmZMY04QRmreaKQqcbouopZa0ATVTLqylpdQZJo-iU8IaxthEVYxP0aac7jE0PrQaX17QyG-xVeJReGfnvizMbeed_yUqUNeHTLHD2LBD8wwgxyd5EDdYqB36MktaEVkyQPGyCPvyH3vsxuLzelqoZ4yI7nqDzHaWDjznJbm-GErmNV-7jzez7Q_d78m-YGfi4A3ZfDuFg5Au1JylAr6k</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1808335961</pqid></control><display><type>article</type><title>Small Glutamine-Rich Tetratricopeptide Repeat-Containing Protein Alpha (SGTA) Ablation Limits Offspring Viability and Growth in Mice</title><source>MEDLINE</source><source>Nature Free</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><source>Springer Nature OA Free Journals</source><creator>Philp, Lisa K. ; Day, Tanya K. ; Butler, Miriam S. ; Laven-Law, Geraldine ; Jindal, Shalini ; Hickey, Theresa E. ; Scher, Howard I. ; Butler, Lisa M. ; Tilley, Wayne D.</creator><creatorcontrib>Philp, Lisa K. ; Day, Tanya K. ; Butler, Miriam S. ; Laven-Law, Geraldine ; Jindal, Shalini ; Hickey, Theresa E. ; Scher, Howard I. ; Butler, Lisa M. ; Tilley, Wayne D.</creatorcontrib><description>Small glutamine-rich tetratricopeptide repeat-containing protein α (SGTA) has been implicated as a co-chaperone and regulator of androgen and growth hormone receptor (AR, GHR) signalling. We investigated the functional consequences of partial and full
Sgta
ablation
in vivo
using Cre-lox
Sgta
-null mice.
Sgta
+/−
breeders generated viable
Sgta
−/−
offspring, but at less than Mendelian expectancy. S
gta
−/−
breeders were subfertile with small litters and higher neonatal death (
P
< 0.02). Body size was significantly and proportionately smaller in male and female
Sgta
−/−
(vs WT,
Sgta
+/−
P
< 0.001) from d19. Serum IGF-1 levels were genotype- and sex-dependent. Food intake, muscle and bone mass and adiposity were unchanged in
Sgta
−/−
. Vital and sex organs had normal relative weight, morphology and histology, although certain androgen-sensitive measures such as penis and preputial size and testis descent, were greater in
Sgta
−/−
. Expression of
AR
and its targets remained largely unchanged, although AR localisation was genotype- and tissue-dependent. Generally expression of other TPR-containing proteins was unchanged. In conclusion, this thorough investigation of SGTA-null mutation reports a mild phenotype of reduced body size. The model’s full potential likely will be realised by genetic crosses with other models to interrogate the role of SGTA in the many diseases in which it has been implicated.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep28950</identifier><identifier>PMID: 27358191</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/136 ; 631/208/727 ; 631/443/494 ; 631/45/776 ; 692/163/2743 ; Ablation ; Animals ; Body Size ; Cancer research ; Cell cycle ; Female ; Females ; Health sciences ; Histology ; Humanities and Social Sciences ; Male ; Males ; Medical research ; Mice ; Mice, Knockout ; Molecular Chaperones - genetics ; Molecular Chaperones - metabolism ; multidisciplinary ; Offspring ; Ovaries ; Prostate ; Proteins ; Science ; Survival Analysis</subject><ispartof>Scientific reports, 2016-06, Vol.6 (1), p.28950-28950, Article 28950</ispartof><rights>The Author(s) 2016</rights><rights>Copyright Nature Publishing Group Jun 2016</rights><rights>Copyright © 2016, Macmillan Publishers Limited 2016 Macmillan Publishers Limited</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-9b9588447073c050e7dc5a1efcb0a8613d9ec0ad5460dc8440ba1605b33db9433</citedby><cites>FETCH-LOGICAL-c504t-9b9588447073c050e7dc5a1efcb0a8613d9ec0ad5460dc8440ba1605b33db9433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4928056/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4928056/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27358191$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Philp, Lisa K.</creatorcontrib><creatorcontrib>Day, Tanya K.</creatorcontrib><creatorcontrib>Butler, Miriam S.</creatorcontrib><creatorcontrib>Laven-Law, Geraldine</creatorcontrib><creatorcontrib>Jindal, Shalini</creatorcontrib><creatorcontrib>Hickey, Theresa E.</creatorcontrib><creatorcontrib>Scher, Howard I.</creatorcontrib><creatorcontrib>Butler, Lisa M.</creatorcontrib><creatorcontrib>Tilley, Wayne D.</creatorcontrib><title>Small Glutamine-Rich Tetratricopeptide Repeat-Containing Protein Alpha (SGTA) Ablation Limits Offspring Viability and Growth in Mice</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Small glutamine-rich tetratricopeptide repeat-containing protein α (SGTA) has been implicated as a co-chaperone and regulator of androgen and growth hormone receptor (AR, GHR) signalling. We investigated the functional consequences of partial and full
Sgta
ablation
in vivo
using Cre-lox
Sgta
-null mice.
Sgta
+/−
breeders generated viable
Sgta
−/−
offspring, but at less than Mendelian expectancy. S
gta
−/−
breeders were subfertile with small litters and higher neonatal death (
P
< 0.02). Body size was significantly and proportionately smaller in male and female
Sgta
−/−
(vs WT,
Sgta
+/−
P
< 0.001) from d19. Serum IGF-1 levels were genotype- and sex-dependent. Food intake, muscle and bone mass and adiposity were unchanged in
Sgta
−/−
. Vital and sex organs had normal relative weight, morphology and histology, although certain androgen-sensitive measures such as penis and preputial size and testis descent, were greater in
Sgta
−/−
. Expression of
AR
and its targets remained largely unchanged, although AR localisation was genotype- and tissue-dependent. Generally expression of other TPR-containing proteins was unchanged. In conclusion, this thorough investigation of SGTA-null mutation reports a mild phenotype of reduced body size. The model’s full potential likely will be realised by genetic crosses with other models to interrogate the role of SGTA in the many diseases in which it has been implicated.</description><subject>631/136</subject><subject>631/208/727</subject><subject>631/443/494</subject><subject>631/45/776</subject><subject>692/163/2743</subject><subject>Ablation</subject><subject>Animals</subject><subject>Body Size</subject><subject>Cancer research</subject><subject>Cell cycle</subject><subject>Female</subject><subject>Females</subject><subject>Health sciences</subject><subject>Histology</subject><subject>Humanities and Social Sciences</subject><subject>Male</subject><subject>Males</subject><subject>Medical research</subject><subject>Mice</subject><subject>Mice, Knockout</subject><subject>Molecular Chaperones - genetics</subject><subject>Molecular Chaperones - metabolism</subject><subject>multidisciplinary</subject><subject>Offspring</subject><subject>Ovaries</subject><subject>Prostate</subject><subject>Proteins</subject><subject>Science</subject><subject>Survival Analysis</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNplkUFrGzEQhUVpaYKTQ_9AEfSSFDaRVit7dSkY0zoFl5TE7VVotbOxglbaSNqW3PvDI-PUuI0uI9A3b97oIfSOkgtKWH0ZAwxlLTh5hY5LUvGiZGX5-uB-hE5jvCf58FJUVLxFR-WM8ZoKeoz-3PbKWry0Y1K9cVDcGL3Ba0hBpWC0H2BIpgV8AwOoVCy8S8o44-7w9-ATGIfndtgofHa7XM_P8byxKhnv8Mr0JkV83XVxCFv8p1GNsSY9YuVavAz-d9rg3P7NaDhBbzplI5w-1wn68eXzenFVrK6XXxfzVaE5qVIhGsHruqpmZMY04QRmreaKQqcbouopZa0ATVTLqylpdQZJo-iU8IaxthEVYxP0aac7jE0PrQaX17QyG-xVeJReGfnvizMbeed_yUqUNeHTLHD2LBD8wwgxyd5EDdYqB36MktaEVkyQPGyCPvyH3vsxuLzelqoZ4yI7nqDzHaWDjznJbm-GErmNV-7jzez7Q_d78m-YGfi4A3ZfDuFg5Au1JylAr6k</recordid><startdate>20160630</startdate><enddate>20160630</enddate><creator>Philp, Lisa K.</creator><creator>Day, Tanya K.</creator><creator>Butler, Miriam S.</creator><creator>Laven-Law, Geraldine</creator><creator>Jindal, Shalini</creator><creator>Hickey, Theresa E.</creator><creator>Scher, Howard I.</creator><creator>Butler, Lisa M.</creator><creator>Tilley, Wayne D.</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160630</creationdate><title>Small Glutamine-Rich Tetratricopeptide Repeat-Containing Protein Alpha (SGTA) Ablation Limits Offspring Viability and Growth in Mice</title><author>Philp, Lisa K. ; Day, Tanya K. ; Butler, Miriam S. ; Laven-Law, Geraldine ; Jindal, Shalini ; Hickey, Theresa E. ; Scher, Howard I. ; Butler, Lisa M. ; Tilley, Wayne D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-9b9588447073c050e7dc5a1efcb0a8613d9ec0ad5460dc8440ba1605b33db9433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>631/136</topic><topic>631/208/727</topic><topic>631/443/494</topic><topic>631/45/776</topic><topic>692/163/2743</topic><topic>Ablation</topic><topic>Animals</topic><topic>Body Size</topic><topic>Cancer research</topic><topic>Cell cycle</topic><topic>Female</topic><topic>Females</topic><topic>Health sciences</topic><topic>Histology</topic><topic>Humanities and Social Sciences</topic><topic>Male</topic><topic>Males</topic><topic>Medical research</topic><topic>Mice</topic><topic>Mice, Knockout</topic><topic>Molecular Chaperones - genetics</topic><topic>Molecular Chaperones - metabolism</topic><topic>multidisciplinary</topic><topic>Offspring</topic><topic>Ovaries</topic><topic>Prostate</topic><topic>Proteins</topic><topic>Science</topic><topic>Survival Analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Philp, Lisa K.</creatorcontrib><creatorcontrib>Day, Tanya K.</creatorcontrib><creatorcontrib>Butler, Miriam S.</creatorcontrib><creatorcontrib>Laven-Law, Geraldine</creatorcontrib><creatorcontrib>Jindal, Shalini</creatorcontrib><creatorcontrib>Hickey, Theresa E.</creatorcontrib><creatorcontrib>Scher, Howard I.</creatorcontrib><creatorcontrib>Butler, Lisa M.</creatorcontrib><creatorcontrib>Tilley, Wayne D.</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Philp, Lisa K.</au><au>Day, Tanya K.</au><au>Butler, Miriam S.</au><au>Laven-Law, Geraldine</au><au>Jindal, Shalini</au><au>Hickey, Theresa E.</au><au>Scher, Howard I.</au><au>Butler, Lisa M.</au><au>Tilley, Wayne D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Small Glutamine-Rich Tetratricopeptide Repeat-Containing Protein Alpha (SGTA) Ablation Limits Offspring Viability and Growth in Mice</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2016-06-30</date><risdate>2016</risdate><volume>6</volume><issue>1</issue><spage>28950</spage><epage>28950</epage><pages>28950-28950</pages><artnum>28950</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Small glutamine-rich tetratricopeptide repeat-containing protein α (SGTA) has been implicated as a co-chaperone and regulator of androgen and growth hormone receptor (AR, GHR) signalling. We investigated the functional consequences of partial and full
Sgta
ablation
in vivo
using Cre-lox
Sgta
-null mice.
Sgta
+/−
breeders generated viable
Sgta
−/−
offspring, but at less than Mendelian expectancy. S
gta
−/−
breeders were subfertile with small litters and higher neonatal death (
P
< 0.02). Body size was significantly and proportionately smaller in male and female
Sgta
−/−
(vs WT,
Sgta
+/−
P
< 0.001) from d19. Serum IGF-1 levels were genotype- and sex-dependent. Food intake, muscle and bone mass and adiposity were unchanged in
Sgta
−/−
. Vital and sex organs had normal relative weight, morphology and histology, although certain androgen-sensitive measures such as penis and preputial size and testis descent, were greater in
Sgta
−/−
. Expression of
AR
and its targets remained largely unchanged, although AR localisation was genotype- and tissue-dependent. Generally expression of other TPR-containing proteins was unchanged. In conclusion, this thorough investigation of SGTA-null mutation reports a mild phenotype of reduced body size. The model’s full potential likely will be realised by genetic crosses with other models to interrogate the role of SGTA in the many diseases in which it has been implicated.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>27358191</pmid><doi>10.1038/srep28950</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 631/136 631/208/727 631/443/494 631/45/776 692/163/2743 Ablation Animals Body Size Cancer research Cell cycle Female Females Health sciences Histology Humanities and Social Sciences Male Males Medical research Mice Mice, Knockout Molecular Chaperones - genetics Molecular Chaperones - metabolism multidisciplinary Offspring Ovaries Prostate Proteins Science Survival Analysis |
| title | Small Glutamine-Rich Tetratricopeptide Repeat-Containing Protein Alpha (SGTA) Ablation Limits Offspring Viability and Growth in Mice |
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