Influence of Culture Media on Detection of Carbapenem Hydrolysis by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

In this study, we evaluated the influence of distinct bacterial growth media on detection of carbapenemase hydrolysis by matrix-assisted laser desorption ionization-time of flight mass spectrometry. False-negative results were observed for OXA-25-, OXA-26-, and OXA-72-producing Acinetobacter baumann...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of clinical microbiology 2016-07, Vol.54 (7), p.1896-1898
Hauptverfasser:	Ramos, Ana Carolina, Carvalhaes, Cecília Godoy, Cordeiro-Moura, Jhonatha Rodrigo, Rockstroh, Anna Carolina, Machado, Antonia Maria Oliveira, Gales, Ana Cristina
Format:	Artikel
Sprache:	eng
Schlagworte:	Acinetobacter baumannii - enzymology Bacterial Proteins - analysis Bacteriology beta-Lactamases - analysis Carbapenems - metabolism Culture Media - chemistry False Negative Reactions Hydrolysis Sensitivity and Specificity Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	In this study, we evaluated the influence of distinct bacterial growth media on detection of carbapenemase hydrolysis by matrix-assisted laser desorption ionization-time of flight mass spectrometry. False-negative results were observed for OXA-25-, OXA-26-, and OXA-72-producing Acinetobacter baumannii isolates grown on MacConkey agar medium. The other culture media showed 100% sensitivity and 100% specificity for detecting carbapenemase.
ISSN:	0095-1137 1098-660X
DOI:	10.1128/JCM.00749-16