Comparison of DOT-ELISA and Standard-ELISA for Detection of the Vibrio cholerae Toxin in Culture Supernatants of Bacteria Isolated from Human and Environmental Samples

A comparison of DOT-ELISA and Standard-ELISA was made for detection of Vibrio cholerae toxin in culture supernatants of bacteria isolated from human and environmental samples. A total of 293 supernatants were tested in a double blind assay. A correlation of 100 % was obtained between both techniques...

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Veröffentlicht in:	Indian journal of microbiology 2016-09, Vol.56 (3), p.379-382
Hauptverfasser:	Meza-Lucas, Antonio, Pérez-Villagómez, María-Fernanda, Martínez-López, José-Patricio, García-Rodea, Ricardo, Martínez-Castelán, María-Guadalupe, Escobar-Gutiérrez, Alejandro, de-la-Rosa-Arana, Jorge-Luis, Villanueva-Zamudio, Altagracia
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Biomedical and Life Sciences Cholera Drinking water Epidemics Laboratories Life Sciences Medical Microbiology Microbiology Seafood Sewage Short Communication Toxins Waterborne diseases
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Zusammenfassung:	A comparison of DOT-ELISA and Standard-ELISA was made for detection of Vibrio cholerae toxin in culture supernatants of bacteria isolated from human and environmental samples. A total of 293 supernatants were tested in a double blind assay. A correlation of 100 % was obtained between both techniques. The cholera toxin was found in 20 Inaba and 3 Ogawa strains. Positive samples were from seafood (17 samples), potable water (1 sample) and sewage (5 samples). The DOT-ELISA was useful as the standard-ELISA to confirm the presence of cholera toxin in the environmental samples.
ISSN:	0046-8991 0973-7715
DOI:	10.1007/s12088-016-0596-2