Gonadotropin-Releasing Hormone Stimulate Aldosterone Production in a Subset of Aldosterone-Producing Adenoma

We aimed to detect novel genes associated with G protein-coupled receptors (GPCRs) in aldosterone-producing adenoma (APA) and elucidate the mechanisms underlying aldosterone production.Microarray analysis targeting GPCR-associated genes was conducted using APA without known mutations (APA-WT) sample...

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Veröffentlicht in:	Medicine (Baltimore) 2016-05, Vol.95 (20), p.e3659-e3659
Hauptverfasser:	Kishimoto, Rui, Oki, Kenji, Yoneda, Masayasu, Gomez-Sanchez, Celso E., Ohno, Haruya, Kobuke, Kazuhiro, Itcho, Kiyotaka, Kohno, Nobuoki
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenoma - genetics Adenoma - metabolism Adrenal Gland Neoplasms - genetics Adrenal Gland Neoplasms - metabolism Adult Aldosterone - biosynthesis Cell Line, Tumor Cross-Sectional Studies Female G Protein-Coupled Inwardly-Rectifying Potassium Channels - genetics Gene Expression - genetics Genotype Gonadotropin-Releasing Hormone - pharmacology Humans Male Middle Aged Mutation Observational Study Oligonucleotide Array Sequence Analysis Receptors, LH - genetics Receptors, LHRH - genetics RNA, Messenger - metabolism
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Zusammenfassung:	We aimed to detect novel genes associated with G protein-coupled receptors (GPCRs) in aldosterone-producing adenoma (APA) and elucidate the mechanisms underlying aldosterone production.Microarray analysis targeting GPCR-associated genes was conducted using APA without known mutations (APA-WT) samples (n = 3) and APA with the KCNJ5 mutation (APA-KCNJ5; n = 3). Since gonadotropin-releasing hormone receptor (GNRHR) was the highest expression in APA-WT by microarray analysis, we investigated the effect of gonadotropin-releasing hormone (GnRH) stimulation on aldosterone production.The quantitative polymerase chain reaction assay results revealed higher GNRHR expression levels in APA-WT samples those in APA-KCNJ5 samples (P
ISSN:	0025-7974 1536-5964
DOI:	10.1097/MD.0000000000003659