Sesterterpene ophiobolin biosynthesis involving multiple gene clusters in Aspergillus ustus
Terpenoids are the most diverse and abundant natural products among which sesterterpenes account for less than 2%, with very few reports on their biosynthesis. Ophiobolins are tricyclic 5–8–5 ring sesterterpenes with potential pharmaceutical application. Aspergillus ustus 094102 from mangrove rizhos...
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Veröffentlicht in: | Scientific reports 2016-06, Vol.6 (1), p.27181-27181, Article 27181 |
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Sprache: | eng |
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Zusammenfassung: | Terpenoids are the most diverse and abundant natural products among which sesterterpenes account for less than 2%, with very few reports on their biosynthesis. Ophiobolins are tricyclic 5–8–5 ring sesterterpenes with potential pharmaceutical application.
Aspergillus ustus
094102 from mangrove rizhosphere produces ophiobolin and other terpenes. We obtained five gene cluster knockout mutants, with altered ophiobolin yield using genome sequencing and
in silico
analysis, combined with
in vivo
genetic manipulation. Involvement of the five gene clusters in ophiobolin synthesis was confirmed by investigation of the five key terpene synthesis relevant enzymes in each gene cluster, either by gene deletion and complementation or
in vitro
verification of protein function. The results demonstrate that ophiobolin skeleton biosynthesis involves five gene clusters, which are responsible for C15, C20, C25, and C30 terpenoid biosynthesis. |
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ISSN: | 2045-2322 2045-2322 |
DOI: | 10.1038/srep27181 |