Genomic Analysis Using a Yeast Artificial Chromosome Library with Mouse DNA Inserts
A yeast artificial chromosome library with mouse genomic DNA inserts has been constructed. The library encompasses a 2.5-fold coverage of the mouse genome, with an average insert size of 250 kilobases. The screening strategy uses the polymerase chain reaction on pooled DNAs prepared from individuall...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1992-03, Vol.89 (6), p.2456-2460 |
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| container_issue | 6 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 89 |
| creator | Rossi, Janice M. Burke, David T. Jimmy C. M. Leung Koos, David S. Chen, Hsiuchen Tilghman, Shirley M. |
| description | A yeast artificial chromosome library with mouse genomic DNA inserts has been constructed. The library encompasses a 2.5-fold coverage of the mouse genome, with an average insert size of 250 kilobases. The screening strategy uses the polymerase chain reaction on pooled DNAs prepared from individually stored clones. The usefulness of the library for chromosome walking was illustrated by constructing a 600-kilobase-long contig of DNA surrounding Hba-ps4, a DNA marker that is tightly linked to the fused (Fu) locus on chromosome 17. |
| doi_str_mv | 10.1073/pnas.89.6.2456 |
| format | Article |
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M. Leung ; Koos, David S. ; Chen, Hsiuchen ; Tilghman, Shirley M.</creator><creatorcontrib>Rossi, Janice M. ; Burke, David T. ; Jimmy C. M. Leung ; Koos, David S. ; Chen, Hsiuchen ; Tilghman, Shirley M.</creatorcontrib><description>A yeast artificial chromosome library with mouse genomic DNA inserts has been constructed. The library encompasses a 2.5-fold coverage of the mouse genome, with an average insert size of 250 kilobases. The screening strategy uses the polymerase chain reaction on pooled DNAs prepared from individually stored clones. The usefulness of the library for chromosome walking was illustrated by constructing a 600-kilobase-long contig of DNA surrounding Hba-ps4, a DNA marker that is tightly linked to the fused (Fu) locus on chromosome 17.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.89.6.2456</identifier><identifier>PMID: 1347950</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Base Sequence ; Biological and medical sciences ; Cellular biology ; Chromosome Mapping ; Chromosomes ; Chromosomes, Fungal ; Deoxyribonucleic acid ; Diverse techniques ; DNA ; DNA - genetics ; DNA - isolation & purification ; DNA probes ; Exons ; Female ; Fundamental and applied biological sciences. Psychology ; Gels ; Gene Library ; Genetic loci ; Genetic Markers ; Genetic screening ; Genetics ; Genome ; Genomics ; Libraries ; Mice ; Mice, Inbred C57BL ; Molecular and cellular biology ; Molecular Sequence Data ; Oligodeoxyribonucleotides ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Rodents ; Saccharomyces cerevisiae - genetics ; Spleen - physiology ; Yeasts</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1992-03, Vol.89 (6), p.2456-2460</ispartof><rights>Copyright 1992 The National Academy of Sciences of the United States of America</rights><rights>1992 INIST-CNRS</rights><rights>Copyright National Academy of Sciences Mar 15, 1992</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c544t-b196ba819f2fd32e97c665d527f5a291f9d4d14e4dd1bfcffdf6111e0df9181c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/89/6.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2358724$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2358724$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5167788$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1347950$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rossi, Janice M.</creatorcontrib><creatorcontrib>Burke, David T.</creatorcontrib><creatorcontrib>Jimmy C. M. Leung</creatorcontrib><creatorcontrib>Koos, David S.</creatorcontrib><creatorcontrib>Chen, Hsiuchen</creatorcontrib><creatorcontrib>Tilghman, Shirley M.</creatorcontrib><title>Genomic Analysis Using a Yeast Artificial Chromosome Library with Mouse DNA Inserts</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>A yeast artificial chromosome library with mouse genomic DNA inserts has been constructed. The library encompasses a 2.5-fold coverage of the mouse genome, with an average insert size of 250 kilobases. The screening strategy uses the polymerase chain reaction on pooled DNAs prepared from individually stored clones. The usefulness of the library for chromosome walking was illustrated by constructing a 600-kilobase-long contig of DNA surrounding Hba-ps4, a DNA marker that is tightly linked to the fused (Fu) locus on chromosome 17.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cellular biology</subject><subject>Chromosome Mapping</subject><subject>Chromosomes</subject><subject>Chromosomes, Fungal</subject><subject>Deoxyribonucleic acid</subject><subject>Diverse techniques</subject><subject>DNA</subject><subject>DNA - genetics</subject><subject>DNA - isolation & purification</subject><subject>DNA probes</subject><subject>Exons</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>Gene Library</subject><subject>Genetic loci</subject><subject>Genetic Markers</subject><subject>Genetic screening</subject><subject>Genetics</subject><subject>Genome</subject><subject>Genomics</subject><subject>Libraries</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Molecular and cellular biology</subject><subject>Molecular Sequence Data</subject><subject>Oligodeoxyribonucleotides</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Rodents</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Spleen - physiology</subject><subject>Yeasts</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcGPEyEYxYnRrHX16kkTYoy3qcAAA4mXprrrJlUPugdPhGFgSzMzVGDU_e-XprVWY-KJw_u97_seD4CnGM0xaurX21GnuZBzPieU8XtghpHEFacS3QczhEhTCUroQ_AopQ1CSDKBzsAZrmkjGZqBz5d2DIM3cDHq_jb5BK-TH2-ghl-tThkuYvbOG697uFzHMIQUBgtXvo063sIfPq_hhzAlC99-XMCrMdmY02PwwOk-2SeH9xxcX7z7snxfrT5dXi0Xq8owSnPVYslbLbB0xHU1sbIxnLOOkcYxTSR2sqMdppZ2HW6dca5zHGNsUeckFtjU5-DNfu52agfbGTvmqHu1jX4ox6mgvfpTGf1a3YTvigreNMX-6mCP4dtkU1aDT8b2vR5tiaQaIqigiP4XxJxQShkq4Iu_wE2YYvnYpAjCRGBKRIHme8jEkFK07ngwRmpXqdpVqoRUXO0qLYbnpzF_4_sOi_7yoOtkdO-iHo1PR4zhElaIkxS78b_U4xrlpr7P9mc-2fdPsOjP9vom5RCPAKmZaAit7wBKbMtO</recordid><startdate>19920315</startdate><enddate>19920315</enddate><creator>Rossi, Janice M.</creator><creator>Burke, David T.</creator><creator>Jimmy C. 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Leung</creator><creator>Koos, David S.</creator><creator>Chen, Hsiuchen</creator><creator>Tilghman, Shirley M.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19920315</creationdate><title>Genomic Analysis Using a Yeast Artificial Chromosome Library with Mouse DNA Inserts</title><author>Rossi, Janice M. ; Burke, David T. ; Jimmy C. 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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1992-03, Vol.89 (6), p.2456-2460 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_48677 |
| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Base Sequence Biological and medical sciences Cellular biology Chromosome Mapping Chromosomes Chromosomes, Fungal Deoxyribonucleic acid Diverse techniques DNA DNA - genetics DNA - isolation & purification DNA probes Exons Female Fundamental and applied biological sciences. Psychology Gels Gene Library Genetic loci Genetic Markers Genetic screening Genetics Genome Genomics Libraries Mice Mice, Inbred C57BL Molecular and cellular biology Molecular Sequence Data Oligodeoxyribonucleotides Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Rodents Saccharomyces cerevisiae - genetics Spleen - physiology Yeasts |
| title | Genomic Analysis Using a Yeast Artificial Chromosome Library with Mouse DNA Inserts |
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