Genomic Analysis Using a Yeast Artificial Chromosome Library with Mouse DNA Inserts

A yeast artificial chromosome library with mouse genomic DNA inserts has been constructed. The library encompasses a 2.5-fold coverage of the mouse genome, with an average insert size of 250 kilobases. The screening strategy uses the polymerase chain reaction on pooled DNAs prepared from individuall...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1992-03, Vol.89 (6), p.2456-2460
Hauptverfasser:	Rossi, Janice M., Burke, David T., Jimmy C. M. Leung, Koos, David S., Chen, Hsiuchen, Tilghman, Shirley M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Biological and medical sciences Cellular biology Chromosome Mapping Chromosomes Chromosomes, Fungal Deoxyribonucleic acid Diverse techniques DNA DNA - genetics DNA - isolation & purification DNA probes Exons Female Fundamental and applied biological sciences. Psychology Gels Gene Library Genetic loci Genetic Markers Genetic screening Genetics Genome Genomics Libraries Mice Mice, Inbred C57BL Molecular and cellular biology Molecular Sequence Data Oligodeoxyribonucleotides Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Rodents Saccharomyces cerevisiae - genetics Spleen - physiology Yeasts
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Zusammenfassung:	A yeast artificial chromosome library with mouse genomic DNA inserts has been constructed. The library encompasses a 2.5-fold coverage of the mouse genome, with an average insert size of 250 kilobases. The screening strategy uses the polymerase chain reaction on pooled DNAs prepared from individually stored clones. The usefulness of the library for chromosome walking was illustrated by constructing a 600-kilobase-long contig of DNA surrounding Hba-ps4, a DNA marker that is tightly linked to the fused (Fu) locus on chromosome 17.
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.89.6.2456