Oil-free hyaluronic acid matrix for serial femtosecond crystallography

The grease matrix was originally introduced as a microcrystal-carrier for serial femtosecond crystallography and has been expanded to applications for various types of proteins, including membrane proteins. However, the grease-based matrix has limited application for oil-sensitive proteins. Here we...

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Veröffentlicht in:	Scientific reports 2016-04, Vol.6 (1), p.24484-24484, Article 24484
Hauptverfasser:	Sugahara, Michihiro, Song, Changyong, Suzuki, Mamoru, Masuda, Tetsuya, Inoue, Shigeyuki, Nakane, Takanori, Yumoto, Fumiaki, Nango, Eriko, Tanaka, Rie, Tono, Kensuke, Joti, Yasumasa, Kameshima, Takashi, Hatsui, Takaki, Yabashi, Makina, Nureki, Osamu, Numata, Keiji, Iwata, So
Format:	Artikel
Sprache:	eng
Schlagworte:	631/535/1266 639/624/1020/1087 Crystallography Crystallography, X-Ray - methods Endopeptidase K Endopeptidase K - chemistry Grease Humanities and Social Sciences Hyaluronic acid Hyaluronic Acid - chemistry Lysozyme Membrane proteins multidisciplinary Muramidase - chemistry Oils - chemistry Proteinase Proteins Science Skin care products
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Zusammenfassung:	The grease matrix was originally introduced as a microcrystal-carrier for serial femtosecond crystallography and has been expanded to applications for various types of proteins, including membrane proteins. However, the grease-based matrix has limited application for oil-sensitive proteins. Here we introduce a grease-free, water-based hyaluronic acid matrix. Applications for proteinase K and lysozyme proteins were able to produce electron density maps at 2.3-Å resolution.
ISSN:	2045-2322 2045-2322
DOI:	10.1038/srep24484