Autophagy is dispensable for B-cell development but essential for humoral autoimmune responses

To gain new insight into the role of B-cell autophagy, we generated two novel mouse models deficient for the autophagy-related gene ( Atg ) 5 , one from the outset pro-B cell stage ( Atg5 f/− Mb1 cre) and the other in mature B cells only ( Atg5 f/− CD21 cre). We show that autophagy is dispensable for pro- to pre-B cell transition, but necessary at a basal level to maintain normal numbers of peripheral B cells. It appears non-essential for B-cell activation under B-cell receptor stimulation but required for their survival after lipopolysaccharide stimulation that drives plasmablast differentiation and for specific IgM production after immunization. Results obtained using Atg5 f/− CD21 cre × C57BL/6 lpr/lpr autoimmune-prone mice show that B-cell autophagy is involved in the maintenance of anti-nuclear antibody secretion, elevated number of long-lived plasma cells, and sustains IgG deposits in the kidneys. Thus, treatments specifically targeting autophagy might be beneficial in systemic autoimmune diseases.