Diagnosis of Trypanosoma evansi in bovines by indirect ELISA

The diagnosis of Trypanosoma evansi in animals with low parasitaemia is hampered by low diagnostic sensitivity of traditional detection methods. The present study was undertaken with an objective to improve the diagnostic tools for detection of antibodies against T. evansi infection using indirect e...

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Veröffentlicht in:	Journal of parasitic diseases 2016-03, Vol.40 (1), p.141-144
Hauptverfasser:	Sivajothi, S., Rayulu, V. C., Malakondaiah, P., Sreenivasulu, D.
Format:	Artikel
Sprache:	eng
Schlagworte:	antibodies antigens buffaloes cattle diagnostic sensitivity enzyme-linked immunosorbent assay Health Promotion and Disease Prevention India Infectious Diseases Medicine Medicine & Public Health Original Original Article parasitemia Trypanosoma evansi trypanosomiasis
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Beschreibung
Zusammenfassung:	The diagnosis of Trypanosoma evansi in animals with low parasitaemia is hampered by low diagnostic sensitivity of traditional detection methods. The present study was undertaken with an objective to improve the diagnostic tools for detection of antibodies against T. evansi infection using indirect enzyme-linked immunosorbent assay (ELISA) in bovines. The optimum concentration of antigen, test sera and conjugate were determined as 5 µg per well, 1:10 and 1: 6,000 dilutions, respectively. Among 320 cattle and 382 buffaloes examined in different parts of Rayalaseema region of Andhra Pradesh for T. evansi infection, 36.12 and 31.87 percent were found positive by indirect ELISA, respectively.
ISSN:	0971-7196 0975-0703
DOI:	10.1007/s12639-014-0465-z