Determination of Sequences Required for Human Endogenous Retrovirus K Transduction and Its Recognition by Foreign Retroviral Virions

Sequences necessary for transduction of human endogenous retrovirus (HERV)-Kcon, a consensus of the HERV-K(HML-2) family, were analyzed and found to reside in the leader/gag region. They act in an orientation-dependent way and consist of at least two sites working together. Having defined these sequ...

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Veröffentlicht in:	Journal of virology 2015-12, Vol.90 (6), p.3243-3246
Hauptverfasser:	Erlwein, Otto, Sweeney, Nathan P, de Leon, Raffaele, Wills, Gillian, Robinson, Mark J, McClure, Myra O
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell Line DNA, Viral - genetics Endogenous Retroviruses - genetics Gene Products, gag - genetics Genetic Therapy - methods Genetic Vectors HIV-1 - genetics Humans Leukemia Virus, Murine - genetics Transduction, Genetic Virus-Cell Interactions
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Zusammenfassung:	Sequences necessary for transduction of human endogenous retrovirus (HERV)-Kcon, a consensus of the HERV-K(HML-2) family, were analyzed and found to reside in the leader/gag region. They act in an orientation-dependent way and consist of at least two sites working together. Having defined these sequences, we exploited this information to produce a simple system to investigate to what extent virions of HERV-Kcon, murine leukemia virus, and HIV-1 have the ability to transduce each other's genomes, leading to potential contamination of gene therapy vectors.
ISSN:	0022-538X 1098-5514
DOI:	10.1128/JVI.02731-15