Neuromuscular electrical stimulation acutely mobilizes endothelial progenitor cells in critically ill patients with sepsis

Background Endothelial progenitor cells (EPCs) have been suggested to constitute a restoration index of the disturbed endothelium in ICU patients. Neuromuscular electric stimulation (NMES) is increasingly employed in ICU to prevent comorbidities such as ICU-acquired weakness, which is related to endothelial dysfunction. The role of NMES to mobilize EPCs has not been investigated yet. The purpose of this study was to explore the NMES-induced effects on mobilization of EPCs in septic ICU patients. Methods Thirty-two septic mechanically ventilated patients (mean ± SD, age 58 ± 14 years) were randomized to one of the two 30-min NMES protocols of different characteristics: a high-frequency (75 Hz, 6 s on–21 s off) or a medium-frequency (45 Hz, 5 s on–12 s off) protocol both applied at maximally tolerated intensity. Blood was sampled before and immediately after the NMES sessions. Different EPCs subpopulations were quantified by cytometry markers CD34 + /CD133 + /CD45 − , CD34 + /CD133 + /CD45 − /VEGFR 2 + and CD34 + /CD45 − /VEGFR 2 + . Results Overall, CD34 + /CD133 + /CD45 − EPCs increased from 13.5 ± 10.2 to 20.8 ± 16.9 and CD34 + /CD133 + /CD45 − /VEGFR 2 + EPCs from 3.8 ± 5.2 to 6.4 ± 8.5 cells/10 6 enucleated cells (mean ± SD, p