An automated method for the microbiological assay of serum pyridoxal

A fully automated method for the measurement of serum pyridoxal has been developed. Acid phosphatase was used for dephosphorylation and precipitation of the serum proteins was not required. A chloramphenicol-resistant strain of L. casei was used as the test organism and this removed the need for ste...

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Veröffentlicht in:	Journal of clinical pathology 1973-11, Vol.26 (11), p.871-874
Hauptverfasser:	Davis, R. E., Smith, B. K., Curnow, D. H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acid Phosphatase Adolescent Adult Age Factors Aged Autoanalysis Biological Assay Blood Preservation Child Chloramphenicol Drug Resistance, Microbial Female Humans Lactobacillus casei - metabolism Male Methods Middle Aged Pyridoxal Phosphate - blood Sex Factors
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Beschreibung
Zusammenfassung:	A fully automated method for the measurement of serum pyridoxal has been developed. Acid phosphatase was used for dephosphorylation and precipitation of the serum proteins was not required. A chloramphenicol-resistant strain of L. casei was used as the test organism and this removed the need for sterilization. The method gives highly reproducible results, and is suitable for population and institutional studies.
ISSN:	0021-9746 1472-4146
DOI:	10.1136/jcp.26.11.871