A fluorimetric and enzymatic method for the estimation of serum total bile acids

A fluorimetric and enzymatic method for the estimation of serum total bile acids

The enzymatic technique using 3α-hydroxysteroid dehydrogenase for determining bile acids in blood has been modified by measuring the reduced nicotinamide adenine dinucleotide fluorimetrically. The increased sensitivity attained enables the concentration of total bile acids in serum to be estimated u...

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Veröffentlicht in:Journal of clinical pathology 1970-10, Vol.23 (7), p.594-598
Hauptverfasser: Murphy, G. M., Billing, Barbara H., Baron, D. N.
Format: Artikel
Sprache:eng
Schlagworte:
Bile Acids and Salts - blood
Female
Fluorometry
Humans
Hydroxysteroid Dehydrogenases
Jaundice - blood
Male
Methods
NAD
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Zusammenfassung:The enzymatic technique using 3α-hydroxysteroid dehydrogenase for determining bile acids in blood has been modified by measuring the reduced nicotinamide adenine dinucleotide fluorimetrically. The increased sensitivity attained enables the concentration of total bile acids in serum to be estimated using 3 ml for normal subjects and 1 ml for jaundiced patients. The range of normal values in serum was found to be 0-4·7 μmol/litre for males and 1·0-8·2 μmol/litre for females.
ISSN:0021-9746
1472-4146
DOI:10.1136/jcp.23.7.594