Evaluation of an Immunochromatographic Assay for Rapid Detection of Penicillin-Binding Protein 2a in Human and Animal Staphylococcus intermedius Group, Staphylococcus lugdunensis, and Staphylococcus schleiferi Clinical Isolates

The performance of a rapid penicillin-binding protein 2a (PBP2a) detection assay, the Alere PBP2a culture colony test, was evaluated for identification of PBP2a-mediated beta-lactam resistance in human and animal clinical isolates of Staphylococcus intermedius group, Staphylococcus lugdunensis, and...

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Veröffentlicht in:	Journal of clinical microbiology 2016-03, Vol.54 (3), p.745-748
Hauptverfasser:	Arnold, A R, Burnham, C-A D, Ford, B A, Lawhon, S D, McAllister, S K, Lonsway, D, Albrecht, V, Jerris, R C, Rasheed, J K, Limbago, B, Burd, E M, Westblade, L F
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacteriology Humans Immunochromatography - methods Immunochromatography - standards Penicillin-Binding Proteins - metabolism Peptide Synthases - metabolism Reproducibility of Results Sensitivity and Specificity Staphylococcal Infections - microbiology Staphylococcus intermedius - isolation & purification Staphylococcus intermedius - metabolism Staphylococcus lugdunensis - isolation & purification Staphylococcus lugdunensis - metabolism
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Zusammenfassung:	The performance of a rapid penicillin-binding protein 2a (PBP2a) detection assay, the Alere PBP2a culture colony test, was evaluated for identification of PBP2a-mediated beta-lactam resistance in human and animal clinical isolates of Staphylococcus intermedius group, Staphylococcus lugdunensis, and Staphylococcus schleiferi. The assay was sensitive and specific, with all PBP2a-negative and PBP2a-positive strains testing negative and positive, respectively.
ISSN:	0095-1137 1098-660X
DOI:	10.1128/JCM.02869-15