Retinol as a cofactor for PKCδ‐mediated impairment of insulin sensitivity in a mouse model of diet‐induced obesity

We previously defined that the mitochondria‐localized PKCδ signaling complex stimulates the conversion of pyruvate to acetyl‐coenzyme A by the pyruvate dehydrogenase complex. We demonstrated in vitro and ex vivo that retinol supplementation enhances ATP synthesis in the presence of the PKCδ signalosome. Here, we tested in vivo if a persistent oversupply of retinol would further impair glucose metabolism in a mouse model of diet‐induced insulin resistance. We crossed mice over‐expressing human retinol‐binding protein (hRBP) under the muscle creatine kinase (MCK) promoter (MCKhRBP) with the PKCδ–/– strain to generate mice with a different status of the PKCδ signalosome and retinoid levels. Mice with a functional PKCδ signalosome and elevated retinoid levels (PKCδ+/+hRBP) developed the most advanced stage of insulin resistance. In contrast, elevation of retinoid levels in mice with inactive PKCδ did not affect remarkably their metabolism, resulting in phenotypic similarity between PKCδ–/– hRBP and PKCδ–/– mice. Therefore, in addition to the well‐defined role of PKCδ in the etiology of metabolic syndrome, we present a novel PKCδ signaling pathway that requires retinol as a metabolic cofactor and is involved in the regulation of fuel utilization in mitochondria. The distinct role in whole‐body energy homeostasis establishes the PKCδ signalosome as a promising target for therapeutic intervention in metabolic disorders.—Shabrova, E., Hoyos, B., Vinogradov, V., Kim, Y.‐K., Wassef, L., Leitges, M., Quadro, L., Hammerling, U., Retinol as a cofactor for PKCδ‐mediated impairment of insulin sensitivity in a mouse model of diet‐induced obesity. FASEB J. 30, 1339–1355 (2016). www.fasebj.org