φXANES: In vivo imaging of metal-protein coordination environments

φXANES: In vivo imaging of metal-protein coordination environments

We have developed an X-ray absorption near edge structure spectroscopy method using fluorescence detection for visualizing in vivo coordination environments of metals in biological specimens. This approach, which we term fluorescence imaging XANES (φXANES), allows us to spatially depict metal-protei...

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Veröffentlicht in:Scientific reports 2016-02, Vol.6 (1), p.20350-20350, Article 20350
Hauptverfasser: James, Simon A., Hare, Dominic J., Jenkins, Nicole L., de Jonge, Martin D., Bush, Ashley I., McColl, Gawain
Format: Artikel
Sprache:eng
Schlagworte:
140/146
631/45/321/1155
639/638/11/942
639/766/747
Animals
Caenorhabditis elegans - metabolism
Coordination Complexes - chemistry
Humanities and Social Sciences
Image Processing, Computer-Assisted - methods
Iron - chemistry
Metalloproteins - chemistry
Models, Molecular
multidisciplinary
Organometallic Compounds - chemistry
Oxidation-Reduction
Science
X-Ray Absorption Spectroscopy - methods
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Zusammenfassung:We have developed an X-ray absorption near edge structure spectroscopy method using fluorescence detection for visualizing in vivo coordination environments of metals in biological specimens. This approach, which we term fluorescence imaging XANES (φXANES), allows us to spatially depict metal-protein associations in a native, hydrated state whilst avoiding intrinsic chemical damage from radiation. This method was validated using iron-challenged Caenorhabditis elegans to observe marked alterations in redox environment.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep20350