Casilio： a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation and genomic labeling

The CRISPR-Cas9 system has recently been widely adopted in genome editing due to its simplicity [1-3]. Nuclease-deficient mutant dCas9 protein can be fused to effector domains and the fusion proteins can be guided by sgRNAs to genomic sites to regulate gene expression or label chromosomes [4-10]. However, only one type of effector is applied in most experiments due to the exclu- sive sgRNA：Cas9 pairing.