Automated enzymatic fluorometric method for the determination of pyruvic and lactic acids in blood

Automated enzymatic fluorometric method for the determination of pyruvic and lactic acids in blood

A simple automated fluorometric method for the estimation of lactate and pyruvate is described. Analyses can be performed at 60/hour and can conveniently determine pyruvate and lactate in the ranges 0-5 mg. and 0-30 mg/100 ml. The methods give a high degree of analytical precision and good yields fr...

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Veröffentlicht in:Journal of clinical pathology 1968-03, Vol.21 (2), p.171-174
1. Verfasser: Cramp, D. G.
Format: Artikel
Sprache:eng
Schlagworte:
Autoanalysis
Fluorometry
Humans
L-Lactate Dehydrogenase
Lactates - blood
Methods
NAD
Pyruvates - blood
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Zusammenfassung:A simple automated fluorometric method for the estimation of lactate and pyruvate is described. Analyses can be performed at 60/hour and can conveniently determine pyruvate and lactate in the ranges 0-5 mg. and 0-30 mg/100 ml. The methods give a high degree of analytical precision and good yields from recovery experiments.
ISSN:0021-9746
1472-4146
DOI:10.1136/jcp.21.2.171