Does the Capsule Interfere with Performance of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Cryptococcus neoformans and Cryptococcus gattii?

We described the impact of the capsule size for Cryptococcus neoformans and Cryptococcus gattii identification at the species level by Bruker matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). After experimental capsule size modulation, we observed that redu...

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Veröffentlicht in:	Journal of clinical microbiology 2016-02, Vol.54 (2), p.474-477
Hauptverfasser:	Thomaz, Danilo Y, Grenfell, Rafaella C, Vidal, Monica S M, Giudice, Mauro C, Del Negro, Gilda M B, Juliano, Luiz, Benard, Gil, de Almeida Júnior, João N
Format:	Artikel
Sprache:	eng
Schlagworte:	Cryptococcosis - diagnosis Cryptococcosis - microbiology Cryptococcus gattii - classification Cryptococcus gattii - genetics Cryptococcus neoformans Cryptococcus neoformans - classification Cryptococcus neoformans - genetics Genotype Humans Mycological Typing Techniques - methods Mycology Sensitivity and Specificity Serogroup Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
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Zusammenfassung:	We described the impact of the capsule size for Cryptococcus neoformans and Cryptococcus gattii identification at the species level by Bruker matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). After experimental capsule size modulation, we observed that reducing the capsule size resulted in improved identification by Bruker MALDI-TOF MS across all of the reference strains analyzed.
ISSN:	0095-1137 1098-660X
DOI:	10.1128/JCM.02635-15