Raman spectroscopy-based identification of nosocomial outbreaks of the clonal bacterium Escherichia coli

DNA-based techniques are frequently used to confirm the relatedness of putative outbreak isolates. These techniques often lack the discriminatory power when analyzing closely related microbes such as E. coli . Here the value of Raman spectroscopy as a typing tool for E. coli in a clinical setting wa...

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Veröffentlicht in:	European journal of clinical microbiology & infectious diseases 2016-01, Vol.35 (1), p.83-87
Hauptverfasser:	Kusters, J. G., van Leeuwen, W. B., Maquelin, K., Blok, H. E. M., Willemse, H. F. M., de Graaf-Miltenburg, L. A. M., Fluit, A. C., Troelstra, A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibiotics Bacterial Typing Techniques - methods Bioinformatics Biomedical and Life Sciences Biomedicine Cross Infection - epidemiology Cross Infection - microbiology Data analysis Disease Outbreaks E coli Epidemics Escherichia coli Escherichia coli - chemistry Escherichia coli - classification Escherichia coli - isolation & purification Escherichia coli Infections - epidemiology Escherichia coli Infections - microbiology Hospitals Humans Intensive care Internal Medicine Medical Microbiology Nosocomial infection Original Original Article Outbreaks Retrospective Studies Spectroscopy Spectrum analysis Spectrum Analysis, Raman - methods
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Zusammenfassung:	DNA-based techniques are frequently used to confirm the relatedness of putative outbreak isolates. These techniques often lack the discriminatory power when analyzing closely related microbes such as E. coli . Here the value of Raman spectroscopy as a typing tool for E. coli in a clinical setting was retrospectively evaluated.
ISSN:	0934-9723 1435-4373
DOI:	10.1007/s10096-015-2511-x