A Jun-Binding Protein Related to a Putative Tumor Suppressor

A λ gt11 cDNA library of chicken embryo fibroblasts was screened with biotinylated Jun protein to identify Jun-binding clones. Eight such clones were isolated; one contains a gene referred to as jif-1 that is homologous to the putative tumor suppressor gene QM. jif-1 codes for a protein of 25 kDa th...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1993-07, Vol.90 (14), p.6726-6730
Hauptverfasser:	Monteclaro, Felipe S., Vogt, Peter K.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Amino acids Animals Avian Proteins Biological and medical sciences Birds Carrier Proteins - genetics Carrier Proteins - metabolism Chick Embryo DNA Fibroblasts Fundamental and applied biological sciences. Psychology Gene Library Genes Genes, Tumor Suppressor - genetics Leucine Zippers Molecular and cellular biology Molecular genetics Molecular Sequence Data Oligonucleotides Plasmids Plasmids - genetics Polymerase chain reaction Proteins Proto-Oncogene Proteins c-fos - metabolism Proto-Oncogene Proteins c-jun - metabolism Regulatory Sequences, Nucleic Acid Repression Response elements Ribosomal Proteins Sequence Analysis, DNA Sequence Homology, Amino Acid Transactivation Transcription. Transcription factor. Splicing. Rna processing Transcriptional Activation Tumor Suppressor Proteins Tumors
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Zusammenfassung:	A λ gt11 cDNA library of chicken embryo fibroblasts was screened with biotinylated Jun protein to identify Jun-binding clones. Eight such clones were isolated; one contains a gene referred to as jif-1 that is homologous to the putative tumor suppressor gene QM. jif-1 codes for a protein of 25 kDa that binds to the leucine zipper of viral and cellular Jun. The Jif-1 protein also binds to itself. Jif-1 does not contain a leucine zipper, and it does not bind to the 12-O-tetradecanoylphorbol 13-acetate response element DNA sequence. Complex formation of Jif-1 with Jun inhibits DNA binding and reduces transactivation by Jun. Addition of Fos protein to Jun-Jif-1 complexes restores DNA-binding activity. These observations suggest that Jif-1 is a negative regulator of Jun.
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.90.14.6726