Molecular breeding of lignin-degrading brown-rot fungus Gloeophyllum trabeum by homologous expression of laccase gene

The basidiomycete Gloeophyllum trabeum KU-41 can degrade Japanese cedar wood efficiently. To construct a strain better suited for biofuel production from Japanese cedar wood, we developed a gene transformation system for G. trabeum KU-41 using the hygromycin phosphotransferase-encoding gene ( hpt )...

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Veröffentlicht in:AMB Express 2015-12, Vol.5 (1), p.81-81, Article 81
Hauptverfasser: Arimoto, Misa, Yamagishi, Kenji, Wang, Jianqiao, Tanaka, Kanade, Miyoshi, Takanori, Kamei, Ichiro, Kondo, Ryuichiro, Mori, Toshio, Kawagishi, Hirokazu, Hirai, Hirofumi
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Sprache:eng
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Zusammenfassung:The basidiomycete Gloeophyllum trabeum KU-41 can degrade Japanese cedar wood efficiently. To construct a strain better suited for biofuel production from Japanese cedar wood, we developed a gene transformation system for G. trabeum KU-41 using the hygromycin phosphotransferase-encoding gene ( hpt ) as a marker. The endogenous laccase candidate gene ( Gtlcc3 ) was fused with the promoter of the G. trabeum glyceraldehyde-3-phosphate dehydrogenase-encoding gene and co-transformed with the hpt -bearing pAH marker plasmid. We obtained 44 co-transformants, and identified co-transformant L#61, which showed the highest laccase activity among all the transformants. Moreover, strain L#61 was able to degrade lignin in Japanese cedar wood-containing medium, in contrast to wild-type G. trabeum KU-41 and to a typical white-rot fungus Phanerochaete chrysosporium . By using strain L#61, direct ethanol production from Japanese cedar wood was improved compared to wild type. To our knowledge, this study is the first report of the molecular breeding of lignin-degrading brown-rot fungus and direct ethanol production from softwoods by co-transformation with laccase overproduction constructs.
ISSN:2191-0855
2191-0855
DOI:10.1186/s13568-015-0173-9