Molecular breeding of lignin-degrading brown-rot fungus Gloeophyllum trabeum by homologous expression of laccase gene
The basidiomycete Gloeophyllum trabeum KU-41 can degrade Japanese cedar wood efficiently. To construct a strain better suited for biofuel production from Japanese cedar wood, we developed a gene transformation system for G. trabeum KU-41 using the hygromycin phosphotransferase-encoding gene ( hpt )...
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Veröffentlicht in: | AMB Express 2015-12, Vol.5 (1), p.81-81, Article 81 |
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Sprache: | eng |
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Zusammenfassung: | The basidiomycete
Gloeophyllum trabeum
KU-41 can degrade Japanese cedar wood efficiently. To construct a strain better suited for biofuel production from Japanese cedar wood, we developed a gene transformation system for
G. trabeum
KU-41 using the hygromycin phosphotransferase-encoding gene (
hpt
) as a marker. The endogenous laccase candidate gene (
Gtlcc3
) was fused with the promoter of the
G. trabeum
glyceraldehyde-3-phosphate dehydrogenase-encoding gene and co-transformed with the
hpt
-bearing pAH marker plasmid. We obtained 44 co-transformants, and identified co-transformant L#61, which showed the highest laccase activity among all the transformants. Moreover, strain L#61 was able to degrade lignin in Japanese cedar wood-containing medium, in contrast to wild-type
G. trabeum
KU-41 and to a typical white-rot fungus
Phanerochaete chrysosporium
. By using strain L#61, direct ethanol production from Japanese cedar wood was improved compared to wild type. To our knowledge, this study is the first report of the molecular breeding of lignin-degrading brown-rot fungus and direct ethanol production from softwoods by co-transformation with laccase overproduction constructs. |
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ISSN: | 2191-0855 2191-0855 |
DOI: | 10.1186/s13568-015-0173-9 |